Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

论数学作文训练在教学中的作用

阐述了数学作文训练对巩固数学知识基础、培育数学思想、优化数学思维品质、培养和发展数学能力有明显的作用,它是提高数学素质的一种新的教学模式,并且对培养和提高学生的整体素质也有积极的影响.     

Development of Full Automatic External Welding Machine for Annular Welds of Pipeline

To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided.     

桥头跳车的成因及处理方法

桥头跳车会增加行车风险并且会大大增加每年的养护费用．本文作者根据多年的养护管理及施工经验，对桥头跳车的成因从设计、施工及运营使用几方面进行了分析并提出了几种处理方法，尤其是对桥头搭板和土工格栅网两种目前工程界常用方法从设计机理到施工实践进行了比较详细的论述，同时对设计机理方面引入了相应的数学模型．     

制备介孔复合环境材料的酸洗刻蚀工艺

探索了一种制备介孔复合环境材料的方法--破坏式造孔与有机复合相结合,使最终产品可大量吸附污染水体中的各种污染物并具有一定的杀菌功能.该方法通过酸洗刻蚀,达到了去除原材料中的杂质和刻蚀孔径的目的,所制备的中间产品24h吸湿率为原材料的2倍,染料吸附量为原材料的1.6倍.     

极低硫钢的精炼脱硫动力学模型

通过对影响脱硫反应限制性环节的分析,将持续接触和瞬时接触两种反应模式综合考虑,建立了精炼脱硫动力学模型,并通过实验室"极低硫钢精炼脱硫"实验数据对模型进行验证.结果表明,本模型的预测结果与实验结果比较吻合.     

峰谷电价体制下东北输油管网日输油优化研究

东北原油长输管网是中国规模最大的地区性原油运输系统，就该管网基于峰谷电价体制下的运行现状，建立了线性规划数学模型，提出了日输油计划优化问题，并以铁秦线为例进行分析；初步揭示了峰谷电价体制下日输油计划方式的一些基本规律， 表明在该体制下东北管网具有一定的节能降耗潜力。但该方式在一定程度上受到管道本身运行特性的约束，与热油管道相比它更适合于等温管道。     

Spectroscopic study on the photophysical properties of chlorine substituted tetraphenylporphyrin-histidine and its zinc(Ⅱ)complexes

As the structural analogues of (bacterio)chlorophylls in photosynthetic organisms, porphyrins play an important role in building up artificial photosynthetic systems[13]. In natural photosynthetic pigment-protein complexes,certain amino acid residuals modulate the reactions ofcharge transfer and energy transfer via coordination andother types of interactions with chlorophyll molecules.For instance, in higher-plant photosynthetic reaction cen-ter PSⅡ, the oxidization-reduction system consistin…     

2,3-二溴甲基-5,6-二氰基吡嗪的合成新方法

以2,3-二甲基-5,6-二氰基吡嗪为原料,在AIBN的引发下,用NBS进行自由基溴化反应,合成了标题产物,其结构经熔点,MS,1HNMR,UV等予以确证,从而为该物质的合成探索了一条新途径.     

CRP复合材料板复合型裂纹扩展方向预测理论研究

本文对碳纤维增强的复合材料板裂纹扩展方向预测理论进行了研究。介绍了最新发展起来的复合材料Z断裂理论 ,并通过试验证明了新理论的正确性