全文获取类型
收费全文 | 1375篇 |
免费 | 7篇 |
国内免费 | 16篇 |
专业分类
系统科学 | 9篇 |
丛书文集 | 4篇 |
教育与普及 | 31篇 |
理论与方法论 | 1篇 |
现状及发展 | 623篇 |
研究方法 | 74篇 |
综合类 | 631篇 |
自然研究 | 25篇 |
出版年
2017年 | 9篇 |
2015年 | 9篇 |
2012年 | 23篇 |
2011年 | 29篇 |
2010年 | 10篇 |
2009年 | 37篇 |
2008年 | 33篇 |
2007年 | 48篇 |
2006年 | 24篇 |
2005年 | 26篇 |
2004年 | 55篇 |
2003年 | 28篇 |
2002年 | 32篇 |
2001年 | 80篇 |
2000年 | 58篇 |
1999年 | 71篇 |
1992年 | 30篇 |
1991年 | 19篇 |
1990年 | 16篇 |
1989年 | 15篇 |
1988年 | 20篇 |
1987年 | 11篇 |
1986年 | 12篇 |
1985年 | 24篇 |
1984年 | 14篇 |
1983年 | 11篇 |
1982年 | 11篇 |
1980年 | 18篇 |
1979年 | 31篇 |
1978年 | 20篇 |
1977年 | 19篇 |
1976年 | 23篇 |
1975年 | 32篇 |
1974年 | 20篇 |
1973年 | 28篇 |
1972年 | 19篇 |
1971年 | 44篇 |
1970年 | 35篇 |
1969年 | 31篇 |
1968年 | 37篇 |
1967年 | 33篇 |
1966年 | 33篇 |
1965年 | 22篇 |
1964年 | 16篇 |
1960年 | 9篇 |
1959年 | 14篇 |
1958年 | 24篇 |
1957年 | 21篇 |
1956年 | 13篇 |
1948年 | 8篇 |
排序方式: 共有1398条查询结果,搜索用时 34 毫秒
231.
Nitric oxide release from a single cell measured in situ by a porphyrinic-based microsensor. 总被引:26,自引:0,他引:26
Nitric oxide is an important bioregulatory molecule, being responsible, for example, for activity of endothelium-derived relaxing factor (EDRF). Acute hypertension, diabetes, ischaemia and atherosclerosis are associated with abnormalities of EDRF. Nitric oxide is thought to be a retrograde messenger in the central nervous system. The technology is not yet available for rapid detection of NO released by a single cell in the presence of oxygen and/or nitrite, so the release, distribution and reactivity of endogenous NO in biological systems cannot be analysed. Here we describe a porphyrinic microsensor that we have developed and applied to monitoring NO release in a microsystem. We selectively measured in situ the NO released from a single cell with a response time of less than 10 ms. The microsensor consists of p-type semiconducting polymeric porphyrin and a cationic exchanger (Nafion) deposited on a thermally sharpened carbon fibre with a tip diameter of approximately 0.5 microns. The microsensor, which can be operated in either the amperometric or voltammetric mode, is characterized by a linear response up to 300 microM and a detection limit of 10 nM. Nitric oxide at the level of 10(-20) mols can be detected in a single cell. 相似文献
232.
233.
J Sulston Z Du K Thomas R Wilson L Hillier R Staden N Halloran P Green J Thierry-Mieg L Qiu 《Nature》1992,356(6364):37-41
The long-term goal of this project is the elucidation of the complete sequence of the Caenorhabditis elegans genome. During the first year methods have been developed and a strategy implemented that is amenable to large-scale sequencing. The three cosmids sequenced in this initial phase are surprisingly rich in genes, many of which have mammalian homologues. 相似文献
234.
235.
How to improve the performance of the plasma actuators is a problem that is of great interest to many scientific researchers in the field of electrohydrodynamics.Professor WANG LianZe and his group from the School of Aerospace,Tsinghua University,set out to investigate 相似文献
236.
往过同岩体位于藏东类乌齐地区往过同,岩石类型为斑状中粒黑云母二长花岗岩,黑云母K-Ar同位素年龄为35 Ma,时代为始新世。往过同花岗岩富硅、铝、碱,高钾贫钛,低铁、镁、钙,在SiO2-K2O和K2O-Na2O图解中样品均落在钾玄岩系列区,岩石系列为钾玄质花岗岩。岩石富集大离子亲石元素和轻稀土元素,负Eu异常;在原始地幔蛛网图上Th强烈富集,Sr呈现低谷负异常,高场强元素Nb、Ta、Ti亏损,表现出钾玄岩的地球化学特征。经分析,并应用构造环境判别图解,往过同花岗岩形成于后碰撞的构造环境。 相似文献
237.
238.
The giant electromechanical response in ferroelectric relaxors as a critical phenomenon 总被引:1,自引:0,他引:1
The direct conversion of electrical energy to mechanical work by a material is relevant to a number of applications. This is illustrated by ferroelectric 'relaxors' such as Pb(Mg(1/3)Nb(2/3))O(3)-PbTiO(3) (PMN-PT; refs 5, 6): these materials exhibit a giant electromechanical (piezoelectric) response that is finding use in ultrasonic and medical applications, as well as in telecommunications. The origins of this effect are, however, still unclear. Here we show that the giant electromechanical response in PMN-PT (and potentially other ferroelectric relaxors) is the manifestation of critical points that define a line in the phase diagram of this system. Specifically, in the electric-field-temperature-composition phase diagram of PMN-PT (the composition being varied by changing the PT concentration), a first-order paraelectric-ferroelectric phase transition terminates in a line of critical points where the piezoelectric coefficient is maximum. Above this line, supercritical evolution is observed. On approaching the critical point, both the energy cost and the electric field necessary to induce ferroelectric polarization rotations decrease significantly, thus explaining the giant electromechanical response of these relaxors. 相似文献
239.
Segal E Fondufe-Mittendorf Y Chen L Thåström A Field Y Moore IK Wang JP Widom J 《Nature》2006,442(7104):772-778
240.
Ambati BK Nozaki M Singh N Takeda A Jani PD Suthar T Albuquerque RJ Richter E Sakurai E Newcomb MT Kleinman ME Caldwell RB Lin Q Ogura Y Orecchia A Samuelson DA Agnew DW St Leger J Green WR Mahasreshti PJ Curiel DT Kwan D Marsh H Ikeda S Leiper LJ Collinson JM Bogdanovich S Khurana TS Shibuya M Baldwin ME Ferrara N Gerber HP De Falco S Witta J Baffi JZ Raisler BJ Ambati J 《Nature》2006,443(7114):993-997
Corneal avascularity-the absence of blood vessels in the cornea-is required for optical clarity and optimal vision, and has led to the cornea being widely used for validating pro- and anti-angiogenic therapeutic strategies for many disorders. But the molecular underpinnings of the avascular phenotype have until now remained obscure and are all the more remarkable given the presence in the cornea of vascular endothelial growth factor (VEGF)-A, a potent stimulator of angiogenesis, and the proximity of the cornea to vascularized tissues. Here we show that the cornea expresses soluble VEGF receptor-1 (sVEGFR-1; also known as sflt-1) and that suppression of this endogenous VEGF-A trap by neutralizing antibodies, RNA interference or Cre-lox-mediated gene disruption abolishes corneal avascularity in mice. The spontaneously vascularized corneas of corn1 and Pax6+/- mice and Pax6+/- patients with aniridia are deficient in sflt-1, and recombinant sflt-1 administration restores corneal avascularity in corn1 and Pax6+/- mice. Manatees, the only known creatures uniformly to have vascularized corneas, do not express sflt-1, whereas the avascular corneas of dugongs, also members of the order Sirenia, elephants, the closest extant terrestrial phylogenetic relatives of manatees, and other marine mammals (dolphins and whales) contain sflt-1, indicating that it has a crucial, evolutionarily conserved role. The recognition that sflt-1 is essential for preserving the avascular ambit of the cornea can rationally guide its use as a platform for angiogenic modulators, supports its use in treating neovascular diseases, and might provide insight into the immunological privilege of the cornea. 相似文献