2,3-Dihydroxybiphenyl dioxygenase gene was first discovered in Arthrobacter sp. strain PJ3

Bacterium strain PJ3,isolated from wastewater and identified as Arthrobacter sp. bacterium based on its 16S rDNA gene,could use carbazole as the sole carbon,nitrogen and energy source. The genomic library of strain PJ3 was constructed and a positive clone JM109(pUCW402) was screened out for the expression of dioxygenase by the ability to form yellow ring-fission product. A 2,3-dihydroxybiphenyl dioxygenase(23DHBD) gene of 933 bp was found in the 3360 bp exogenous fragment of pUCW402 by GenSCAN software and BLAST analysis. The phylogenetic analysis showed that 23DHBD from strain PJ3 formed a deep branch separate from a cluster containing most known 23DHBD in GenBank. Southern hybridization confirmed for the first time that the 23DHBD gene was from the genomic DNA of Arthrobacter sp. PJ3. In order to test the gene function,recombinant bacterium BL21(pETW-8) was constructed to express 23DHBD. The expression level in BL21(pETW-8) was highest compared with the recombinant bacteria JM109(pUCW402) and strain PJ3. We observed that 23DHBD was not absolute specific. The enzyme activity was higher with 2,3-dihydroxybiphenyl as a substrate than with catechol. The substrate specificity assay suggested that 23DHBD was essential for cleavage of bi-cyclic aromatic compounds during the course of aromatic compound biodegradation in Arthrobacter sp. strain PJ3.     

Coherent thermal radiation in thin films and its application in the emissivity design of multilayer films

The infrared transmission spectra of a 0.54-μm-thick Ge film and a 20-μm-thick Si film were experimentally measured. As the incident radiation was in the wavelength range from 1.5μm to 10μm, the Ge film demonstrated a strongly spectral coherence. However, thermal radiation of the Ge film was found to be spatially incoherent due to its extreme thinness. The Si film exhibited significantly spectral and spatial coherence. The results confirmed that thermal radiation of a monolayer film could be coherent spectrally and spatially if the film thickness was comparable with the wavelength. The optical characteristic matrix method was applied to calculate the transmission spectra of the Si and Ge film, and the results agreed well with the measurements. This method was further used to analyze two multilayer films composed of five low emissive layers. Their emissivities were found to be highly emissive at a certain zenith angle, and the emissive peak could be controlled by careful selection of film thickness.     

一种基于内容的图像检索方法

论述了基于颜色特征的图像检索和基于纹理特征的图像检索,在此基础上提出了一种利用图像两种内容特征进行图像检索的方法。     

用于冷中子源系统的氦制冷循环方案分析

为向冷中子源装置氢系统提供17．5K的低温冷源，设计了逆布雷顿循环的液氮预冷模式和4种膨胀机预冷模式，并对这5种循环模式进行热力分析和[火用]分析，获得了各循环模式下的主要热力参数并分析比较了各自的热力性质．结果表明：带液氮预冷和前置式并联膨胀机预冷2种模式的热力性能最好，[火用]效率最高；对于膨胀机预冷循环，并联模式优于串联模式，前置式优于后置式．循环系统[火用]损失部位主要在压缩机、膨胀机和换热器，减小这3部分[火用]损失的途径有以下方面：改善循环，减小系统氦的质量流量；提高压缩机的等温效率、膨胀机的等熵效率；改善换热器的内部温度、温差、压力分布及物流分配．本研究为中国先进研究堆冷中子源氦制冷系统的设计提供了数值基础。     

泛克立格法在渗透系数随机场离散中的应用及存在的问题

在分析各种随机场离散方法优缺点的基础上,提出用泛克立格方法对渗透系数随机场进行离散.编制了KRING.FOR程序对渗透系数随机场的离散效果进行了验证,结果表明在实测点所包围区域内部用最小二乘法所求解得到的权系数而进行的克立格估值计算是符合工程要求的.并针对程序调试过程中及计算结果分析中所出现的问题,提出了相应的解决对策.     

植物学野外实习的模式探索

探讨植物学野外实习模式,改革野外实习内容和形式,引导学生主动获得植物学知识的能力,培养学生学习植物学基本研究方法.野外实习结合科研活动,提高学生科学研究意识与科学素质,提高学生的创造能力,提高植物学野外实习教学质量.     

支持向量机在电力变压器故障诊断中的应用

利用支持向量机的学习方法，构建了电力变压器故障诊断模型．该模型将变压器故障分为放电性和过热性两大类，通过统计分析寻求特征量区分类间的故障类型，采用支持向量机识别类内的故障类型，利用基于交叉验证的网格搜索法来确定支持向量机的参数．考虑到变压器油中溶解气体特征空间的紧致性原理，利用模糊C均值聚类算法对所获取的样本进行预选取，有效地解决了确定模型参数时耗时巨大的问题，并一定程度提高了模型的推广能力．实例验证表明，该模型在有限样本情况下，能达到较高的变压器故障判断率，放电性故障样本正确判断率为90．5％，过热性故障样本正确判断率为85．9％，说明该模型具有很好的分类效果和推广能力．     

复合矩阵的Loewner偏序与特征值不等式

讨论了存在Loewner偏序的两矩阵的k级复合矩阵的关系,并将复合矩阵与广义Schur补结合起来,研究矩阵广义Schur补的复合矩阵与复合矩阵广义Schur补之间的Loewner偏序,得到了Ck[（A^＊BA）/α]≤[Ck（A/α）]^＊Ck[B（β′）]Ck（A/α）等结果,并给出相关的特征值与奇异值不等式,推广和改进了近期的相关结果.     

汽车尾灯支架成形的工艺设计与模拟分析

文章利用对称性,采用左右合模工艺,提高材料利用率。通过数值模拟技术对成形过程进行仿真;对成形缺陷及板料变形程度进行了预测;并对毛坯进行了优化,得到了良好的成形质量,最后通过实验对模拟结果进行了验证。该研究提供了毛坯优化方法,并为同类件工艺设计提供借鉴。     

微生物基因芯片鉴定及再生利用

生物芯片是一种重要的生物分子检测方法,在科研和医学应用领域被广泛关注。该文以大肠杆菌和黄单胞菌检测为例研究微生物的基因芯片鉴定方法和生物芯片的再生使用价值,采用一种自主研制的新型微阵列芯片扫描仪对微生物鉴定和生物芯片的重复利用开展理论与实验研究。结果表明基因芯片鉴定微生物是非常可靠的,所设计的生物分子探针与芯片表面修饰分子的结合很牢固,5次重复杂交清洗后依然可以产生良好的荧光信号,从而验证了再生芯片使用的可行性。为了提高生物芯片的重复使用次数,论文还对生物芯片的设计与荧光标记方法进行了分析讨论。