Tyrosine 842 in the activation loop is required for full transformation by the oncogenic mutant FLT3-ITD

The type III receptor tyrosine kinase FLT3 is frequently mutated in acute myeloid leukemia. Oncogenic FLT3 mutants display constitutive activity leading to aberrant cell proliferation and survival. Phosphorylation on several critical tyrosine residues is known to be essential for FLT3 signaling. Among these tyrosine residues, Y842 is located in the so-called activation loop. The position of this tyrosine residue is well conserved in all receptor tyrosine kinases. It has been reported that phosphorylation of the activation loop tyrosine is critical for catalytic activity for some but not all receptor tyrosine kinases. The role of Y842 residue in FLT3 signaling has not yet been studied. In this report, we show that Y842 is not important for FLT3 activation or ubiquitination but plays a critical role in regulating signaling downstream of the receptor as well as controlling receptor stability. We found that mutation of Y842 in the FLT3-ITD oncogenic mutant background reduced cell viability and increased apoptosis. Furthermore, the introduction of the Y842 mutation in the FLT3-ITD background led to a dramatic reduction in in vitro colony forming capacity. Additionally, mice injected with cells expressing FLT3-ITD/Y842F displayed a significant delay in tumor formation, compared to FLT3-ITD expressing cells. Microarray analysis comparing gene expression regulated by FLT3-ITD versus FLT3-ITD/Y842F demonstrated that mutation of Y842 causes suppression of anti-apoptotic genes. Furthermore, we showed that cells expressing FLT3-ITD/Y842F display impaired activity of the RAS/ERK pathway due to reduced interaction between FLT3 and SHP2 leading to reduced SHP2 activation. Thus, we suggest that Y842 is critical for FLT3-mediated RAS/ERK signaling and cellular transformation.     

Two Innovative Coalition Formation Models for Dynamic Task Allocation in Disaster Rescues

This paper addresses the problem of multi-objective coalition formation for task allocation. In disaster rescue, due to the dynamics of environments, heterogeneity and complexity of tasks as well as limited available agents, it is hard for the single-objective and single (task)-to-single (agent) task allocation approaches to handle task allocation in such circumstances. To this end, two multi-objective coalition formation for task allocation models are proposed for disaster rescues in this paper. First, through coalition formation, the proposed models enable agents to cooperatively perform complex tasks that cannot be completed by single agent. In addition, through adjusting the weights of multiple task allocation objectives, the proposed models can employ the linear programming to generate more adaptive task allocation plans, which can satisfy different task allocation requirements in disaster rescue. Finally, through employing the multi-stage task allocation mechanism of the dynamic programming, the proposed models can handle the dynamics of tasks and agents in disaster environments. Experimental results indicate that the proposed models have good performance on coalition formation for task allocation in disaster environments, which can generate suitable task allocation plans according to various objectives of task allocation.     

自动化码头运输设备充电策略优化的仿真研究

随着自动化集装箱码头成为当今码头的发展趋势,AGV(automated guided vehicle) 作为自动化码头中使用最为广泛的水平运输工具,合理制定其充电策略至关重要。针对当前自动化集装箱码头中AGV充电策略的不足,提出一种以离线充电为主在线充电为辅的充电策略,并且针对在线充电站点位置选择问题提出一种利用热区图选择有效在线充电站点的快捷方法。在使用该种充电策略情况下,通过大量仿真实验得出在码头工作任务量不变的前提下可减少AGV数量配置的结果,说明自动化集装箱码头水平搬运效率得到提高,证明该充电策略具有有效性。     

Data-Driven Leader-Follower Output Synchronization for Networked Non-Linear Multi-Agent Systems with Switching Topology and Time-Varying Delays

This paper studies the output synchronization problem for a class of networked non-linear multi-agent systems with switching topology and time-varying delays. To synchronize the outputs, a leader is introduced whose connectivity to the followers varies with time, and a novel data-driven consensus protocol based on model free adaptive control is proposed, where the reference input of each follower is designed to be the time-varying average of the neighboring agents’ outputs. Both the case when the leader is with a prescribed reference input and the case otherwise are considered. The proposed protocol allows for time-varying delays, switching topology, and does not use the agent structure or the dynamics information implicitly or explicitly. Sufficient conditions are derived to guarantee the closed-loop stability, and conditions for consensus convergence are obtained, where only a joint spanning tree is required. Numerical simulations and practical experiments are conducted to demonstrate the effectiveness of the proposed protocol.     

Contours and Tight Clusters

Nested clusters arise independently in graph partitioning and in the study of contours. We take a step toward unifying these two instances of nested clusters. We show that the graph theoretical tight clusters introduced by Dress, Steel, Moulton and Wu in 2010 are a special case of nested clusters associated to contours.     

An empirical investigation on the risk-return relationship of carbon future market

This paper examines the risk-return relationship for the carbon future market during Phases I, II and III of the European Union Emission Trading Scheme (EU ETS). The risk factors derived from the newly developed LSW model, are embedded into a GARCH framework. This new specification is compared with several GARCH-M type models analyzing the risk-return relationship in the carbon market. The results show that the new specification consistently achieves a good fit and possesses superior explanatory power for the European Union Allowance (EUA) data. Some policy suggestions regarding market efficiency are also provided.     

西秦岭中生代花岗岩锆石U-Pb-Lu-Hf同位素特征及地质意义

对西秦岭地区中川岩体、柏家庄岩体和教场坝花岗岩体进行LA-ICP-MS锆石U-Pb同位素年代学分析, 获得其岩浆侵位年龄分别为220±1, 216±6和222±3 Ma, 表明3个岩体均形成于中?晚三叠世。样品的全岩地球化学分析结果表明, 3个岩体具有相似的稀土及微量元素特征, 均表现为显著亏损高场强元素Nb, Ti和P等, 具有明显的右倾式球粒陨石标准化稀土元素配分模式。锆石Lu-Hf同位素分析结果表明, 3个岩体锆石测年样品的ε_Hf(t)值介于?3.31~+1.68之间, 二阶段模式年龄介于1151~1456 Ma之间。岩石成因分析表明, 这些印支期花岗岩体的母岩浆主要来源于新元古代地壳物质的部分熔融。结合岩体形成时代、岩石成因和区域岩浆作用, 认为这些岩体形成于南秦岭与华南板块沿勉略缝合带相碰撞的造山动力学背景, 可能与华南板块的俯冲板片断离有关。     

Systemic Design for Applying the Combined Use of SSM and CDA to Social Practices

In this paper, we propose a framework for applying the combined use of soft systems methodology (SSM) and critical discourse analysis (CDA) based on social semiotic systemic functional linguistics to social practices. In the social practice, ‘participation’ mode of Checkland’s SSM is used for ‘problem-solving’ whilst CDA is used to problematize ‘problem-solving’ processes within the practice. During the meta-process of problematization of our research, we used Churchman’s systems approach and its theory of ‘boundary critique’ in order to explore issues such as governance, inequality of power, and social values within the organization examined for this study. In other words, the process-oriented methodology proposed in this paper consists of two processes: that of ‘problem-solving’ based on SSM and of problematization of naturalized discourse from the perspective of CDA and two phases of boundary critique in social practice. A detailed account of a case study of Korean social enterprise is given to demonstrate how our proposed framework of the combined use of SSM and CDA is applied into practice. Reflecting the outcome of the case study, this paper argues that critical social research from systemic inquiry using what we call ‘process-oriented methodology’ is useful to address some of the social and complex issues related to understanding dynamic relation between power and discourse amongst participants in the social enterprise in Korean contexts.     

On implementing the symbolic preprocessing function over Boolean polynomial rings in Gröbner basis algorithms using linear algebra

Some techniques using linear algebra was introduced by Faugère in F4 to speed up the reduction process during Gr?bner basis computations.These techniques can also be used in fast implementations of F5 and some other signature-based Gr?bner basis algorithms.When these techniques are applied,a very important step is constructing matrices from critical pairs and existing polynomials by the Symbolic Preprocessing function(given in F4).Since multiplications of monomials and polynomials are involved in the Symbolic Preprocessing function,this step can be very costly when the number of involved polynomials/monomials is huge.In this paper,multiplications of monomials and polynomials for a Boolean polynomial ring are investigated and a specific method of implementing the Symbolic Preprocessing function over Boolean polynomial rings is reported.Many examples have been tested by using this method,and the experimental data shows that the new method is very efficient.