Degradation of pyrene and characterization of<Emphasis Type="Italic">Saccharothrix</Emphasis> sp. PYX-6 from the oligotrophic Tianchi Lake in Xinjiang Uygur Autonomous Region,China

Bacterial strain PYX-6 that utilizes anthracene,phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomous Region of China. Its morphology, physiological and biochemical properties, cell wall pattern and G C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 was sequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was named Saccha-rothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilated pyrene for growth and 0.005% of yeast extract stimulated pyrene degradation and assimilation. The optimal pH of cultural medium and the optimal shaking frequency during cultivation were 6-8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp.PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacterium sp. PYR-1.     

双筒液力减振器节流特性试验和仿真研究

为了解减振器的节流特性及阻尼力异常波动的原因,试验和仿真研究了减振器液压变化历程和阀片受力过程.设置子循环能减少计算量,同时会造成液压剧烈波动的假象.经拟合处理,设置子循环的液压速度过程曲线能表示阻尼力速度特性.开阀前,118减振器的节流由常通孔决定;开阀后由阀片与活塞间的缝隙决定.阀片的应力分布明显分为3层,不能认为阀片全面积受均匀分布的液压作用.结果表明:阀片与弹簧座接触方式的转变是造成异常波动的原因.     

Improving the photocatalytic activity of graphitic carbon nitride by thermaltreatment in a high-pressure hydrogen atmosphere

Extending visible light absorption range and suppressing the recombination of photogenerated charge carriers are always important topics in developing efficient solar-driven photocatalysts. In this study, the thermal treatment process at 400 °C in a high-pressure hydrogen atmosphere was applied to modify graphitic carbon nitride. Compared to the normal atmospheric hydrogen treatment process, this process has the merit of producing nitrogen deficient graphitic carbon nitride in high-yield. The optimal photocatalytic activity of modified graphitic carbon nitride was demonstrated by controlling the treatment duration in the hydrogen atmosphere.The changes in the crystal structure, microstructure and optical properties of carbon nitrides were investigated by several characterizations. The relationship between the photocatalytic activity and structures of graphitic carbon nitride was preliminarily established. The results obtained in this study could provide some new ways of improving the activity of graphitic carbon nitride based photocatalyst.     

竖向偏心荷载作用下吸力桶荷载-变形特性非共轴本构数值分析

在竖向偏心荷载作用下建立吸力式桶形基础数值分析模型,采用砂土非共轴弹塑性本构模型研究土体主应力方向旋转和非共轴角度的变化规律,探讨非共轴特性对吸力桶力学特性的影响.主要研究结论表明:在地基变形过程中,非共轴角度呈现先增大后减小的发展趋势.随着偏心距的增加,等效塑性应变在地基中的分布范围逐渐减小,非共轴特性也将减弱,非共轴特性对荷载-变形特性的影响逐渐减弱.对于桶壁端部阻力和桶壁外侧摩擦阻力,非共轴特性的影响随着偏心距的增加而减弱.对于桶体顶部阻力和桶壁内侧摩擦阻力,非共轴特性的影响随着偏心距的增加而逐渐显著.     

基于SWAT的涟水河上游流域非点源污染模拟

以涟水河娄底水文站上游流域为研究区域,应用SWAT模型对该区域的径流、泥沙及非点源氨氮、总磷污染负荷进行模拟计算.将1998年设置为模型启动期,即＂模型预热＂,利用娄底水文站1999～2002年的实测月径流、氨氮及总磷的实测数据进行模型参数确定,运用2003～2005年实测的月径流、氨氮及总磷数据对调参后的模型进行模型验证,验证结果表明模型基本合理可行,可用于涟水河上游流域的非点源污染模拟计算.运用率定后的模型,去除点源污染,重新进行模拟得到最终的非点源污染氨氮、总磷.根据模拟结果对该区域的径流、泥沙、氨氮及总磷污染负荷的时空分布特点进行了分析,为涟水河娄底水文站上游流域非点源污染的综合防治规划提供了参考和依据.     

基于UG的盘形凸轮参数化设计

文章探讨了基于UG的凸轮参数化建模技术,应用表达式工具和规律曲线功能,成功地实现了盘形凸轮的参数化建模,为凸轮机构的运动动力分析等提供了精确的实体模型。     

Ultrafast isomerization dynamics of retinal in bacteriorhodopsin as revealed by femtosecond absorption spectroscopy

A femtosecond （fs） broad-band absorption apparatus was used to measure the early photoisomerlzation process of bacteriorhodopain＇s （BR） photocycle to reveal the character of the important Intermediate of J625 and to obtain a deeper understanding of the role of photoisomerizaUon in BR photocycle. Two time constants of 0.5 pa （95%） and 2.0 pa （5%） were brought out by global fitting on thirty curves In the nser-lnfrared reglon. We suggest that the first time constant results from the decay of I480 intermediate, and the longer component might be associated with BR isomer. The global analysis over 450, 540, 630, 710 and 870 nm traces identified two time constants, -0.5 and -3 pa. The slower component can be extracted from the processes of both J625→BR568 （540 nm） and J625→K590 （630 nm）, suggesting J-intermediate takes a partial cis configuration. The obvious negative feature in early delay time of 700- 760 nm reglona was attributed to the radiative transition （stimulated emission） from the Franck-Condon actlve conflguratlon along the isomerization potential surface of all-trans-retinal.     

国家治理视野下的中国反腐败国际刑事司法协助研究

自2014 年以来,中国开始高度重视对腐败公职人员的国际追逃和国际追赃工作,在反腐败国际刑事司法协助方面取得了令人瞩目成效,这种成效体现在国际追逃?国际追赃和外交上获得巨大成就.尽管中国反腐败国际刑事司法协助的立法不断完善,并取得了令人瞩目的成绩,但仍然存在许多亟待解决的问题,主要体现在5 个方面:国际追逃重于国际追赃,适用国际刑事司法协助条约展开反腐败国际追逃追赃的频率与已签署的国际条约数量不相称,注重个案而不注重制度完善,缺乏适用于国际多边或双边条约的成熟机制,反腐败相关法律之间存在冲突.     

体积压裂套管剪切变形载荷分析及强度设计

页岩气开发过程中井筒穿越天然裂缝/断层,体积压裂时天然裂缝/断层容易被激活,导致套管剪切变形,套管内径缩小,井下工具无法下入。目前对套管剪切变形条件下的受力分析较少,现有研究主要采用套管Mises应力屈服准则判断套管失效,该准则对于套管大剪切变形问题已不适用。为探究天然裂缝/断层滑移导致的套管剪切变形问题,建立套管-水泥环-地层组合体有限元模型,对位移载荷作用下的套管力学行为进行研究。分析了各因素对套管受力及缩径量的影响,并提出套管缩径量与应力相结合的套管外载设计方法。研究结果表明:套管剪应力及Mises应力随地层滑移量增大而增大,在10 mm滑移量作用下,套管Mises应力已超过屈服强度,建议采用剪切载荷进行强度校核;套管缩径量主要集中在距离滑移面1 m范围内,套管局部区域出现膨胀现象;考虑套管变形量的载荷设计可以有效防止体积压裂过程中套管变形导致压裂工具无法下入的问题。     

基于物理属性与三角形正则度的网格简化算法

针对三维虚拟场景的物理属性显示需求,提出一种带属性的边折叠的三角形网格简化方法.该算法计算折叠代价时以模型边曲率和边上物理属性的增量以及三角形正则度作为权因子,边上物理属性的增量使简化后的模型很好地保留了原模型的物理属性特征,而添加三角形正则度优化了简化后模型三角形的形态.同时还解决了边折叠时导致的拓扑错误,并用多选择技术加快了计算速度.经实验验证和对比分析,证明了算法的有效性与正确性.