Using of the surface plasmon resonance cytosensor for real-time and non-invasive monitoring of cellular effects in living C6 cells induced by PMA

By cell and molecular biology, the traditional life science research has been improved into modern ex-perimental science. Because cell is the basic structural and functional unit of the mysterious life, the study of life phenomena will be ultimately embodied into the study of cell biology. How to acquire a dynamic knowledge of the intricate biomolecular reactivity and process in living cells has become one of the hotspots in recent biological researches. For the complexity of cellular effects,…     

Remote sensing of spatial patterns of urban renewal using linear spectral mixture analysis: A case of central urban area of Shanghai (1997―2000)

It is very important to integrate remote sensing with urban geography that the spectral mixture analysis technique is applied to urban land cover evolvement and its eco-environmental effect. Urban land cover is mainly composed of complicated artificial materials, which is the key factor to limit the development of the spectral mixture analysis technique. There are two main aspects in which the technique of spectral mixture analysis is applied to urban geography： one is to calculate vegetation fraction; the other is to build quantitative model of the urban impervious surface obtained from the combination between high albedo fraction and low albedo fraction. The technique of spectral mixture analysis is firstly applied to study urban renewal pattern, scale and mode which happened in Shanghai City from 1997 to 2000,     

Genetic analysis and molecular mapping of a presenescing leaf gene psll in rice （Oryza sativa L.）

A rice psl1 （presenescing leaf） mutant was obtained from a japonica variety Zhonghua 11 via radiation of ^60Co-γ in M2 generation. Every leaf of the mutant began to wither after it reached the biggest length, while the leaves of the wild variety could keep green for 25--35 d. In this study, genetic analysis and gene mapping were carried out for the mutant identified. The SSR marker analysis showed that the mutant was controlled by a single recessive gene （psl1） located on chromosome 2. Fine mapping of the psl1 locus was conducted with 34 new STS markers developed around psl1 anchored region based on the sequence diversity between Nipponbare and 93-11. The psl1 was further mapped between two STS markers, STS2-19 and STS2-26, with genetic distances of 0.43 and 0.11 cM, respectively, while cosegregated with STS2-25. A BAC contig was found to span the psl1 locus, the region being delimited to 48 kb. This result was very useful for cloning of the psl1 gene.     

Synthetic seismograms of ground motion near earthquake fault using simulated Green’s function method

Seismograms near source fault were synthesized using the hybrid empirical Green＇s function method where the discretely simulated seismic waveforms are used for Green＇s functions instead of the observed waveforms of small earthquakes. The Green＇s function seismic waveforms for small earthquake were calculated by solving wave equation using the pseudo-spectral method with the staggered grid real FFT strategy under a detailed 2-D velocity structure in Kobe region. Magnitude and seismic moment of simulated Green＇s function waveforms were firstly determined by using the relationship between fault length and corner frequency of source spectrum. The simulated Green＇s function waveforms were employed to synthesize seismograms of strong ground motion near the earthquake fault. The synthetic seismograms of the target earthquake were performed based on the model with multiple source rupture processes. The results suggest that synthesized seismograms coincide well with observed seismic waveforms of the 1995 Hyogo-ken Nanbu earthquake. The simulated Green＇s function method is very useful for prediction of the strong ground motion in region without observed seismic waveforms. The present technique spreads application field of the empirical Green＇s function method.     

朊圆酵母Y0401尿酸酶的基本特性研究

研究了尿酸酶分离纯化条件和pH、温度、底物浓度、一些有机和无机试剂对尿酸酶酶促反应速度的影响.研究结果表明,用Triton X-100及巯基乙醇处理细胞悬液(pH 8.5),再用超声波处理破碎细胞效果最佳.硫酸铵沉淀尿酸酶的最佳浓度为75%.用Phenyl-Sepharose CL-4B疏水层析和Xanthine-agarose亲和层析两步纯化步骤可获得理想纯度的酶制剂.在一定的条件下,尿酸酶的最适pH和酸碱稳定pH范围分别为8.5和5.0～12.最适温度为37℃;热稳定性较好,在50℃处理30 min时活性保持90%,在60℃处理30min时活性仍保持80%.在最适条件下,该酶催化尿酸的米氏常数(Km)为3.34×10-5mol/L.一些金属离子和有机化合物对酶活性的影响也进行了研究.     

新时期高校教学档案的收集工作刍议

教学档案是高校档案建设的重要内容,教学档案收集工作是教学业务档案工作的基础,做好高校教学档案收集工作应注意加大自我宣传力度;各级领导的支持;建立健全各种规章制度和奖惩制度;提高人员素质;拓展收集渠道;发挥监督、检查、指导作用;及时、全面、准确掌握教学信息等几个方面。     

指数分布场合下序时应力加速寿命试验的矩估计

讨论了指数分布场合下序进应力加速寿命试验的矩估计问题。     

右群的nil-扩张的半格的半直积

给出了两个半群的半直积为右群的nil-扩张的半格的充要条件．     

考虑界面效应的纳米流体的热传导模型

借鉴图论的有关做法。结合威尔逊空间相互作用模型。构建区域中心城市区位选择的模型，从而试图定量分析封闭和开放两种条件下区域中心城市的适宜区位．从模型中可以看出，在封闭条件下区域中心城市适宜区位趋于区域的地理中心。而在开放条件下区域中心城市适宜区位趋于主导联系方向．作者选取浙江省嘉兴市对模型进行实证分析，分别考察了封闭和开放两种条件下嘉兴区域中心城市适宜区位的变化趋势．     

长白山植物区系研究

长白山植物区系具有以下特征：1）植物种类比较丰富，共计维管植物105科、336属、610种．2）优势现象比较明显．3）本地区属典型的温带分布，地理成分较复杂．4）种的特有现象明显．5）该区系具有古老性．