Specific binding of antigenic peptides to cell-associated MHC class I molecules

T lymphocytes recognize antigen in the form of peptides that associate with specific alleles of class I or class II major histocompatibility (MHC) molecules. By contrast with the clear MHC allele-specific binding of peptides to purified class II molecules purified solubilized class I molecules either bind relatively poorly or show degenerate specificity. Using photo-affinity labelling, we demonstrate here the specific interaction of peptides with cell-associated MHC class I molecules and show that this involves metabolically active processes.     

Characterization of the rough endoplasmic reticulum ribosome-binding activity.

The rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.     

Genotoxic effects of dithane M-45 on the bone marrow cells of mice in vivo

Genotoxic effects of dithane M-45 were studied on the bone marrow cells of male albino mice (Lacca strain) in vivo. Different doses (30 mg, 40 mg and 300 mg/kg b.wt) of dithane M-45 were injected intraperitoneally and their effects were investigated after time intervals of 1, 2, 5 and 10 days. The chromosomal aberrations observed in the bone marrow cells of male mice after treatment with dithane M-45 were fragments, rings, dicentric chromosomes, terminal chromatid deletions, chromatid gaps and breaks. In addition to these chromosomal aberrations, physiological effects such as uneven stretching of chromatin material, end-to-end chromosomal associations, exchange configurations, clumping, stickiness and centromeric associations were also observed.     

提高PLC教学效果之方法的探求

《可编程控制器》作为一门课程正逐渐被各大学所采纳,本文结合作者多年的教学经验总结了几种常用的教学方法,并加以分析比较。     

秋水仙素诱导青虾次级卵母细胞二倍体初探

以青虾卵巢为材料，采用秋水仙素诱导多倍体次级卵母细胞．经滴片试验，确立此实验最佳滴片高度为1．5cm，通过设置5个秋水仙素浓度(0．08～0．40mg／mL)及3个作用时间(6～24h)，观察发生染色体加倍的细胞相对数目．实验结果表明：在使用秋水仙素的浓度为0．08～0．40mg／mL，处理6～24h时都可以获得多倍体卵细胞．在秋水仙素浓度是0．32mg／mL，处理6h可得到最高二倍体突变率(32．58％)．还测试了0．32mg／mL COM作用6h时ATPase活性与生理盐水对照组的变化，结果显示COM会在一定程度上影响细胞的新陈代谢．但是50％左右的成活率表明，这种诱导突变的方法仍具有一定的应用价值．     

庞德诗作中的女性形象解读

伊兹拉·庞德是美国二十世纪初现代派诗人的代表，意象派诗歌的领袖人物，一生致力于诗歌的创新和探索。人们对他的研究主要集中于他的意象派诗歌的技巧以及他对儒家思想的探索。我们用生态女性主义的批评理论，研究庞德诗歌中的女性形象，可以为研究庞德对女性的看法提供崭薪的视角。     

市场的稳定性取决于人心

传统的市场经济的金科玉律——供求关系影响价格,价格围绕价值上下波动,使许多人误认为市场的稳定性也是由供求关系或价格决定。但是利用蛛网模型可得出结论:市场的稳定性取决于人心,即人们对商品的敏感程度。     

在线信息产品捆绑定价研究

基于在线信息产品交易各方的互动关系,考虑网络接入商与信息产品生产商各自市场垄断力的对比,研究了两者进行价格决策的先后顺序对信息产品捆绑的最终价格和对信息产品厂商采用捆绑销售策略的影响.研究结果表明当两者同时进行价格决策时,两者均分市场利润;当两者进行序贯博弈时,先决策价格者将获得先动者优势,且消费者在此情形下的总支付将大于当两者同时决策时的总支付;信息产品厂商采用何种捆绑策略与其决策顺序无关,仅与被捆绑产品的捆绑价值参数相关.