二维金纳米微粒有序阵列的制备

对于用十二烷基硫醇包覆的金纳米微粒，用Langmuir-Blodgett(LB)技术制备了二维金纳米微粒有序阵列．在单纯的硫醇包覆的金纳米微粒形成的LB膜中，由于硫醇分子之间的疏水相互作用，容易导致金纳米微粒的自组织而在LB膜中形成缺陷．为了改善金纳米微粒的成膜性能和提高金纳米微粒阵列的有序性，正十二醇作为添加剂和润滑剂加入到金纳米微粒的氯仿溶液中与金纳米微粒一起形成LB膜．用透射电子显微镜对金纳米微粒的二维阵列进行了表征．结果表明，正十二醇的加入可以有效地减少用LB技术制备的二维金纳米微粒阵列中的缺陷，提高金纳米微粒阵列的有序性．     

智能化装载机载重检测设计

通过对装载机作业时的转矩进行分析,适当简化相关参数,建立起一个基本的动力学模型.根据这个模型,采用单片机80C196KB,设计了装载机载重量检测的实现方案.根据装载机故障信息的自身特点,基于GSM(global standard for mobile communications)模块TC35,实现装载机与远程控制中心之间的双向无线通讯.最后给出相关的软件设计,主要分析了其中几段关键的程序流程.通过上述软硬件的设计,本方案实现了对装载机载重量的实时检测,并对过载等故障信息进行处理.由此显著地提高了装载机的可靠性和稳定性.     

基于TDI的网络安全存储系统研究与实现

提出了传输层网络安全存储系统的体系结构和实现方法 ,将用户管理、访问控制、数据加密和审计日志等安全措施集中到普通网络存储系统中 ,并重点对系统中基于TDI的过滤驱动程序原理和实现进行了分析 .该系统为提高目前网络存储系统的安全性提供了一种方便安全、管理集中、高效可靠的解决方案     

基于消息中间件的网络协同编辑环境框架

给出了利用消息中间件和事件抽象技术来实现文本协同编辑环境的实现框架 ,并在此框架的基础上实现了基于MBus中间件的共享WPS协同编辑系统 .基于此框架可以在网络多媒体会议系统中实现各种可编辑文档的共享和协同编辑 .此框架通过实现的协同文本编辑环境验证了实现网络会议系统中各种可编辑文档协同共享编辑的可能性和可靠性 ,并且能够进一步完善视频会议的功能并增加会议的真实感和沉浸感     

A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)

Severe Acute Respiratory Syndrome (SARS) is a newly identified infectious disease[1—5]. The global outbreak of SARS has been threatening the health of people worldwide and has killed 353 people and infected more than 5462 in 27 countries, as reported by WHO on April 29, 2003 (http://www.who.int/csr/sarscountry/en). Although it has been recognized that a variant of virus from the family of coronavirus might be the candidate pathogen of SARS[1—5], its identity as the unique pathogen sti…     

Genetic Diversity in Populations of Sepiella maindroni Using 16S rRNA Gene Sequence Analysis

Part of the 16S rRNA gene is amplified with PCR and sequenced for 5 populations of common Chinese cuttlefish Sepiella maindroni:three from the South China Sea,one from East China Sea and one from Japan.The result shows that a total of 5 nucleotide positions are found to have gaps or insertions of base pairs among these individuals,and 13 positions are examined to be variable in all the sequences,which range from 494 to 509 base pairs.All of the individuals are grouped into 7 haplotypes (h1-h7).No marked genetic difference is osberved among those populations.All of the individuals from Nagasaki belong to h1 and the h3 haplotype is found only in the coastal waters of China.A→←G transition in Nucleotide 255 is suggested to be taken as a kind of genetic marker to identify the populations distributed in East-South China Sea and the Nagasaki waters of Japan.     

A UDDI Based Shared Resources Management Mechanism in Virtual Enetrprises .

Service and information resources are two kinds of major shared resources in virtual enterprises, where, service resources include third party‘s service resources and enterprise‘s legacy service resources, and information resources include database resources and file resources. Since service and information resources have distributed, autonomic, remotely bound as well as some other similar characteristics, an implementation mechanism on managing shared resources based on UDDI are presented. XML instances are used to describe resources authorization information, WSDLbased special XML instance represents information resource metadata, and these XML instancesare referenced by tModel for realizing the purpose of managing shared resources metadata based on UDDI.     

N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

具有破产概率的企业股利贴现复合增长模型

企业具有破产概率对企业的内在价值有直接影响。在假定企业具有破产概率的条件下,建立破产分别出现在不同阶段时的两个股利贴现模型,可为投资者提供评价有破产可能的企业股票价值的理论依据。     

长江口流速和悬沙垂线规律研究

根据长江口的实测水流流速、悬沙含量及其级配资料，分析了它们变化的规律性，并用通用公式进行了验证．从研究结果可以看出：实测资料与现有理论和经验公式的拟合程度较好；在相对水深η=0.4处的流速、悬沙含量及其级配与垂线平均值基本一致，因而可用η=0.4处的流速u0.4与含沙量S0.4来计算单宽输沙率．