Using of the surface plasmon resonance cytosensor for real-time and non-invasive monitoring of cellular effects in living C6 cells induced by PMA

By cell and molecular biology, the traditional life science research has been improved into modern ex-perimental science. Because cell is the basic structural and functional unit of the mysterious life, the study of life phenomena will be ultimately embodied into the study of cell biology. How to acquire a dynamic knowledge of the intricate biomolecular reactivity and process in living cells has become one of the hotspots in recent biological researches. For the complexity of cellular effects,…     

Insecticidal activity of residual Bt protein at the second trophic level

Since the first commercial release of transgenic crop expressing genes from Bacillus thuringiensis (Bt), there have been concerns about its potential impact on the environment. Research has focused on the ecological effects from second exposure to Bt prot…     

Some evidence of drying trend over northern China from 1951 to 2004

The surface wetness index, Palmer drought sererity index and the retrieval of soil moisture over China were calculated using monthly precipitation and monthly mean surface air temperature. Based on the contrast analysis of the variation of the above three indices and precipitation, the dry/wet spatio-temporal pattern of northern China in the last 54 years was revealed, and the evidence of drying trend over northern China was analyzed, especially. The results show the following four facts: (1) The drying trend is the main characteristic of the eastern part of Northwest China and the central part of North China since the 1980s and it was enhanced in the last 15 years mainly due to the precipitation decrease and the temperature increase; (2) During the last 54 years, there was only one dry/wet shift at the interdecadal scale occurring in the eastern part of Northwest China and the central part of North China in the late 1970s, which was related to 1977/1978 global abrupt change, whereas there were three shifts in Northeast China, one was in the mid 1990s and the other two were in 1965 and 1983, respectively; (3) Unlike the variation trend of other subregions of northern China, the western part of Northwest China is currently located in a relatively wetting period, which is weak-ened due to the temperature increase; (4) The extreme drought frequency is obviously increasing in the eastern part of Northwest China, the central part of North China and Northeast China since the 1980s, which is closely related to the precipitation decrease and temperature increase in these subregions.     

反相高效液相色谱法测定附子有效成分含量的方法研究

运用RP-HPLC测定不同地区附子药材中3种酯型生物碱———新乌头碱、乌头碱和次乌头碱的含量.实验以RP-C18色谱柱为固定相,乙腈-0.1%的乙二胺水溶液为流动相进行梯度洗脱.梯度洗脱程序:乙腈和0.1%的乙二胺水溶液的比率0~17 min为46∶54,17~37 min为82∶18;37~50 min为46∶54,流速为1.0mL/min;紫外检测波长为230 nm.新乌头碱、乌头碱和次乌头碱的标准曲线范围分别是5.62×10-6~5.062×10-5mol/L、2.614×10-6~2.614×10     

朊圆酵母Y0401尿酸酶的基本特性研究

研究了尿酸酶分离纯化条件和pH、温度、底物浓度、一些有机和无机试剂对尿酸酶酶促反应速度的影响.研究结果表明,用Triton X-100及巯基乙醇处理细胞悬液(pH 8.5),再用超声波处理破碎细胞效果最佳.硫酸铵沉淀尿酸酶的最佳浓度为75%.用Phenyl-Sepharose CL-4B疏水层析和Xanthine-agarose亲和层析两步纯化步骤可获得理想纯度的酶制剂.在一定的条件下,尿酸酶的最适pH和酸碱稳定pH范围分别为8.5和5.0～12.最适温度为37℃;热稳定性较好,在50℃处理30 min时活性保持90%,在60℃处理30min时活性仍保持80%.在最适条件下,该酶催化尿酸的米氏常数(Km)为3.34×10-5mol/L.一些金属离子和有机化合物对酶活性的影响也进行了研究.     

点插值无网格方法在平板弯曲问题中的应用

点插值方法是近年来发展起来的一种新型无网格方法．运用该方法时，在问题域上离散一系列随机分布的节点，一点的位移值由该点影响域内的节点插值得到．由于插值函数具有Kronecher Delta函数特性，因此可以很方便地施加本质边界条件．根据变分原理得到平板弯曲的点插值无网格控制方程，将其应用于简支方板和地基板的计算中．算例表明该方法是有效的，适用于薄板和厚板的计算．     

新时期高校教学档案的收集工作刍议

教学档案是高校档案建设的重要内容,教学档案收集工作是教学业务档案工作的基础,做好高校教学档案收集工作应注意加大自我宣传力度;各级领导的支持;建立健全各种规章制度和奖惩制度;提高人员素质;拓展收集渠道;发挥监督、检查、指导作用;及时、全面、准确掌握教学信息等几个方面。     

区域中心城市区位选择的模型研究--以嘉兴市为例

借鉴图论的有关做法,结合威尔逊空间相互作用模型,构建区域中心城市区位选择的模型,从而试图定量分析封闭和开放两种条件下区域中心城市的适宜区位.从模型中可以看出,在封闭条件下区域中心城市适宜区位趋于区域的地理中心,而在开放条件下区域中心城市适宜区位趋于主导联系方向.作者选取浙江省嘉兴市对模型进行实证分析,分别考察了封闭和开放两种条件下嘉兴区域中心城市适宜区位的变化趋势.     

长白山植物区系研究

长白山植物区系具有以下特征：1）植物种类比较丰富，共计维管植物105科、336属、610种．2）优势现象比较明显．3）本地区属典型的温带分布，地理成分较复杂．4）种的特有现象明显．5）该区系具有古老性．     

二维siani台球中的量子与经典对应

研究了二维sinai台球的经典与量子的对应，运用闭合轨道理论及定态展开方法计算了傅立叶变换的量子谱．把傅立叶变换后的量子谱中峰的位置与其所对应的经典轨道长度作对照，我们发现两者之间存在着对应关系．为我们理解量子混沌性提出了新的线索．