Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart

Understanding cellular response to environmental stress has broad implications for human disease. AMP-activated protein kinase (AMPK) orchestrates the regulation of energy-generating and -consuming pathways, and protects the heart against ischaemic injury and apoptosis. A role for circulating hormones such as adiponectin and leptin in the activation of AMPK has received recent attention. Whether local autocrine and paracrine factors within target organs such as the heart modulate AMPK is unknown. Here we show that macrophage migration inhibitory factor (MIF), an upstream regulator of inflammation, is released in the ischaemic heart, where it stimulates AMPK activation through CD74, promotes glucose uptake and protects the heart during ischaemia-reperfusion injury. Germline deletion of the Mif gene impairs ischaemic AMPK signalling in the mouse heart. Human fibroblasts with a low-activity MIF promoter polymorphism have diminished MIF release and AMPK activation during hypoxia. Thus, MIF modulates the activation of the cardioprotective AMPK pathway during ischaemia, functionally linking inflammation and metabolism in the heart. We anticipate that genetic variation in MIF expression may impact on the response of the human heart to ischaemia by the AMPK pathway, and that diagnostic MIF genotyping might predict risk in patients with coronary artery disease.     

城市污水深度处理技术方案比选

简要介绍了城市污水的性质,对城市污水处理厂处理后的污水再次深度处理达到国家《景观娱乐用水水质标准》（GB12941—91）中规定的水体达标排放指标提出两种工艺技术方案,并对两种方案在系统组成、出水水质稳定性、运行费用3个方面进行了比选。     

郭守敬的《授时历》和朝鲜的《七政算内篇》

《授时历》被评价为中国历史上最优秀的历法,并且成为明朝《大统历》和朝鲜《七政算内篇》成书的基础。《七政算内篇》是朝鲜世宗时代(1418—1450年)取得的重要天文业绩之一,以《回回历》为基础,与《七政算外篇》一同于1444年编纂成书。《七政算内篇》的历元、天文常数和计算法等基本体系参照《授时历》,应数值则参照了《大统历》的辛巳应数。且把四余添加在推步的项目中,在计算日月食时使用的常数值则参考了元统1384年编纂的《大统历法通轨》,《七政算内篇》汲取了《授时历》和《大统历》的优点。     

Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry

A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme EcoRI and cloned into the phage lambda gtWES.lambda B. The X-derived nature of the recombinants was confirmed by hybridization to rodent/human cell line DNA containing only the human X chromosome. Such libraries will be particularly useful for the investigation of genetic diseases such as Duchenne muscular dystrophy, where the basic defect has not been elucidated, and of neoplasia, where several specific chromosomal anomalies, particularly for the leukaemias, have been identified.     

增强现实中虚拟物体的投影注册算法研究

研究增强现实的关键技术之一的虚拟物体注册技术,使增强现实系统中的增强信息更具有真实感,结合三色基准注册技术,借助三维空间中的坐标系变换和立体投影变换算法,建立增强现实系统中虚拟物体投影注册模型,提出并推导增强现实系统的虚拟物体立体视觉投影注册算法,该算法能够很好地实现已知几何描述的虚拟物体从其自身坐标系到增强现实系统坐标系的转换,并能够生成具有视差的左右眼立体图像,本算法结合视点的三然基准算法完整地实现了用于增强现实的三维注册。     

头孢唑林钠中杂质噻二唑的分离与分析

采用化学法分离得到头孢唑林钠中的杂质噻二唑,用薄层色谱(TLC)和高效液相色谱(HPLC)对分离得到的噻二唑样品初步定性,用红外吸收光谱(IR)对分离得到的噻二唑样品进行结构确认;根据相对保留时间确定其在头孢唑林钠中的残留;依据欧洲药典的方法,用质量分数为1.0 %的头孢唑林钠溶液做对照,以头孢唑林钠供试品溶液中噻二唑的峰面积与质量分数为1.0 %的对照溶液中头孢唑林钠的峰面积比计算噻二唑的含量.结果表明,分离得到的噻二唑是生产工艺过程的残留,所建立的方法适合头孢唑林钠中残留的噻二唑的定性定量分析.进行实际样品分析,其含量在安全限度范围内(质量分数不超过1.0 %),患者可以放心使用.     

一种流媒体代理缓存系统的研究与实现

在参考已有的流媒体代理缓存理论基础上,采用前缀缓存+补丁算法的缓存管理策略,参照流媒体服务器的工作原理,采用RTSP协议作为流传输协议,设计并实现了一种流媒体代理缓存系统.经实验测试该系统提高了系统的字节命中率,进一步减少对网络带宽资源的需求.     

在土木工程专业开设建筑设计课程的思考

阐述了在土木工程专业开设建筑设计选修课的必要性;并通过分析在土木工程专业的建筑设计选修课当中所反映出来的六类问题——不接受没有教材的上课形式、缺乏美学基础、考虑问题不够全面、不会查规范、不会把握进度和抄袭现象;提出了在建筑设计课中相应采用的改进教学方法——自主辅导法、启发教学法、实证教学法、基础和规范性教学法、计划教学法和鼓励教学法,以及实施这些教学法的效果。