Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture

Manipulation or non-physiological embryo culture environments can lead to defective fetal programming in livestock. Our demonstration of reduced fetal methylation and expression of ovine IGF2R suggests pre-implantation embryo procedures may be vulnerable to epigenetic alterations in imprinted genes. This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures.     

基于VIP与VC的多Agent系统

多Agent系统是当前分布式人工智能研究的热点之一.探讨了单个Agent的结构特征以及多Agent系统的运行机制,结合Visual Prolog5.2和Visual C++6.0提出了一种实现多Agent系统的方案.尝试了多Agent系统背景下Choquet积分在综合评判问题中的应用.     

决策树与模糊决策树的比较

对决策树与模糊决策树的异同进行了比较分析.模糊决策树是决策树在模糊环境下的一种推广 ,它作为一种知识表示形式更符合人类的思维.     

Phylogenetic divergence in a desert fish: differentiation of speckled dace within the Bonneville, Lahontan, and upper Snake river basins

Historical events have had a great impact on the biogeography of fishes of western North America. We examined the genetic variation of the speckled dace ( Rhinichthys osculus ) to determine the effects on this species of extensive hydrological changes during the last 10 million years in the Bonneville and Lahontan basins of the Great Basin and the upper Snake River Basin. Eight hundred sixty-nine base pairs of the mitochondrial gene cytochrome b were sequenced from 97 individuals representing 22 populations within these 3 basins, as well as from 2 individuals of longnose dace ( Rhinichthys cataractae ) that served as outgroups. Additionally, 13 speckled dace sequences representing 3 Bonneville populations were used from GenBank. Phylogenetic relationships were reconstructed using maximum parsimony and maximum likelihood criteria. Analysis of molecular variance was used to determine population structure and to estimate the amount of gene flow across the community boundaries. Three distinct clades were reconstructed representing the Lahontan Basin, the northern Bonneville and upper Snake River basins, and the southern Bonneville Basin. Additionally, most of the population structuring was explained by variation among basins (65.33%). Speckled dace demonstrated high genetic variation. As hypothesized, the northern and southern Bonneville specimens formed separate clades; however, the southern Bonneville clade was basal to a sister clade formed by the northern Bonneville/upper Snake River and Lahontan clades. These relationships indicate that Pliocene connections between the Snake, Lahontan, and Bonneville drainages, rather than more recent Pleistocene connections, best explain population structuring in speckled dace.     

苯酚-硫酸法测定藏木香中多糖含量

采用传统的水煮醇沉法从藏木香中提取可溶性多糖,通过Sevag法脱蛋白后,以葡萄糖为对照品,使用苯酚-硫酸法测定多糖含量.结果表明,在波长486nm处测定吸光度,10-100μg/mL范围内吸光度与被测含量之间具有良好的线性关系,藏木香中多糖的含量为64.37%.     

基于FLUENT的渗流场数值模拟分析

传统上渗流问题的模拟都是求解渗透水头的拉普拉斯方程,为了利用CFD软件强大的计算及前后处理功能,通过在一般流体力学控制方程基础上增加渗透阻力的办法,以源项的形式导入FLUENT软件的求解器中实现了对渗流的数值模拟,并采用求解自由面的VOF法进行浸润面的计算.地下河槽及矩形土坝两个算例表明,无论对于渗流量、浸润面还是溢出...