Epigenetic remodeling in colorectal cancer results in coordinate gene suppression across an entire chromosome band

We report a new mechanism in carcinogenesis involving coordinate long-range epigenetic gene silencing. Epigenetic silencing in cancer has always been envisaged as a local event silencing discrete genes. However, in this study of silencing in colorectal cancer, we found common repression of the entire 4-Mb band of chromosome 2q.14.2, associated with global methylation of histone H3 Lys9. DNA hypermethylation within the repressed genomic neighborhood was localized to three separate enriched CpG island 'suburbs', with the largest hypermethylated suburb spanning 1 Mb. These data change our understanding of epigenetic gene silencing in cancer cells: namely, epigenetic silencing can span large regions of the chromosome, and both DNA-methylated and neighboring unmethylated genes can be coordinately suppressed by global changes in histone modification. We propose that loss of gene expression can occur through long-range epigenetic silencing, with similar implications as loss of heterozygosity in cancer.     

Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer

Since the creation of Dolly via somatic cell nuclear transfer (SCNT), more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1%-5%) is that the clones are likely to be derived from adult stem cells. Support for this hypothesis comes from the findings that the reproductive cloning efficiency for embryonic stem cells is five to ten times higher than that for somatic cells as donors and that cloned pups cannot be produced directly from cloned embryos derived from differentiated B and T cells or neuronal cells. The question remains as to whether SCNT-derived animal clones can be derived from truly differentiated somatic cells. We tested this hypothesis with mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells, progenitor cells and granulocytes. We found that cloning efficiency increases over the differentiation hierarchy, and terminally differentiated postmitotic granulocytes yield cloned pups with the greatest cloning efficiency.     

有约束多目标系统的模糊匈牙利决策及其应用

首先将指标值矩阵转化为模糊关系合成矩阵,再将模糊关系合成矩阵与解决指派问题的匈牙利方法结合起来,提出了有约束多目标系统的一种新决策方法——模糊匈牙利法,并建立其模型,该模型可用著名的匈牙利算法来求解。最后应用该法于文献［1～3］中的实例,通过比较,方法简便,效果很好     

人手柔性触觉感知特性

提出了一种新颖的基于弹性梁有效变形长度控制的柔性触觉感知再现方法,并研制了柔性触觉感知装置.通过在该柔性触觉装置上进行实验,对人手的柔性触觉感知特性进行了研究,得出一些结论.刚度越大,分辨率越低,随着刚度的增大,相比较的2个物体间需具有更大的刚度差异才能够分辨.当人们触摸物体时,习惯的次数为2～6次,按压频率主要分布在0.3～1.3 Hz.实验点的刚度越大越难记忆.连续感知的正确率要明显高于不连续感知的正确率.本实验方法简单有效,得出的实验结论不仅可用来改进触觉再现装置的设计,而且为触觉再现技术的研究提供了生理学依据.     

A Novel Cross-Project Software Defect Prediction Algorithm Based on Transfer Learning

Software Defect Prediction(SDP) technology is an effective tool for improving software system quality that has attracted much attention in recent years. However, the prediction of cross-project data remains a challenge for the traditional SDP method due to the different distributions of the training and testing datasets. Another major difficulty is the class imbalance issue that must be addressed in Cross-Project Defect Prediction(CPDP). In this work,we propose a transfer-leaning algorithm(TSboo...     

光催化-生化联合法处理难生物降解废水的研究进展

综述了光催化-生化联合法处理农药废水、印染废水等难生物降解废水的研究进展,展望了光催化-生化联合法处理技术的发展前景,并提出了今后的研究方向。     

疏水蛋白rHGFI与rHFBI的发泡能力研究

将巴斯德毕赤酵母异源表达出的疏水蛋白rHGFI和rHFBI修饰硅化玻璃后进行玻璃表面接触角测定.结果显示:蛋白处理的硅化玻璃表面由疏水变为亲水.蛋白溶液表面张力测定结果表明:rHGFI和rHFBI是表面活性非常强的蛋白,均可使表面张力降低.以常用的食品乳化剂酪蛋白酸钠为对照,检测rHGFI和rHFBI蛋白的发泡能力和泡沫稳定性.0.1%的rHGFI和rHFBI蛋白溶液发泡能力明显高于同浓度的酪蛋白酸钠溶液,rHFBI蛋白溶液的泡沫稳定性最佳.     

DGPS实时引导和跟踪系统

以对空目标寻的系统的外场试验为背景，介绍了一种采用差分GPS定位技术，结合计算机软件而设计的目标实时引导和跟踪系统，并给出了某试验系统的试验实测数据和结果分析。试验证明，该系统工作十分有效，而且使用方便，工作可靠，值得推广。     

特征尺度自动选择边缘分割方法

针对复杂边缘错综交织而不易分割的问题，提出了特征尺度自动选择边缘分割方法.该方法通过定义相邻两层离散尺度空间中边缘点最大偏移量，以及分析极值路径的4种演化方式，得到具有实际意义的边缘点特征尺度.根据边缘线尺度直方图和尺度空间边缘连通性，对交织的边缘进行剥离和合并，得到分割的结果.通过在多幅测试图片上的实验表明，根据尺度范围获得的边缘多尺度表达层次清晰分明，并且按照显著性提取的显著边缘较少出现细碎旁枝与实际显著边缘能较好地吻合，证明提出的边缘分割方法切实有效.

     

非对称信息下讨价还价的动态博弈:以三阶段讨价还价为例

论文以典型的三阶段讨价还价活动为例,将信息的非对称性:如谈判者不同的价格区间信息、不同的心理压力等因素引入讨价还价博弈分析,先研究了非对称压力完全信息下的讨价还价,接着深入探讨了非对称压力非对称信息下的讨价还价活动.证明了情境因素对谈判活动的重要影响,并得出在某些条件下谈判方的保留价格可以在讨价还价中被揭示出来.