Structure of the kinase domain of human RNA-dependent protein kinase with K296R mutation reveals a face-to-face dimer

RNA-dependent protein kinase (PKR) is crucial for the innate immune response, cell growth, proliferation, signal transduction and apoptosis. The activation process of PKR has been studied for many years and is still under debate. To obtain new insight into the mechanism of PKR activation, we solved the crystal structure of a latent mutant of the PKR kinase domain (PKR-KD) in the apo form at a resolution of 2.9 Å. The overall structure of PKR-KD is similar to previously reported structures. Structural analysis revealed a classical back-to-back dimer and a newly defined face-to-face dimer. Analytical ultracentrifugation experiments, electrostatic surface maps and the model of PKR-KD in complex with the eIF2α substrate all support that the face-to-face dimer is more reflective of PKR in solution. Our results provide new information on PKR dimerization and its activation mechanism.     

生态社会主义对我国构建和谐社会的启示

生态社会主义是20世纪80年代以来西方发达国家绿色运动中崛起的一种独树一帜的思潮。尽管生态社会主义的理论和实践具有浓厚的理想主义色彩,但确为社会主义回应全球性问题提供了想像空间,为我们构建社会主义和谐社会提供了一些可资借鉴的启示。     

低比转速离心叶轮内部流动的数值计算

针对目前准三维方法在低比转速离心叶轮的流场计算中的结果不太令人满意的现实情况 ,将奇点分布法用于该领域的计算 ,并经实例计算验证了该方法的可行性 ,且在计算过程中显示出该方法的简单、快捷和结果精确程度高的特点     

型钢-混凝土粘结机理的探讨

对型钢混凝土结构(SRC结构)中型钢-混凝土的粘结机理及影响粘结性能的主要因素进行了分析和探讨,并从混凝土保护层开裂与否的角度根据粘结力的扩散原理,提出了一种型钢-混凝土粘结强度的计算方法,可为型钢混凝土结构中型钢-混凝土粘结性能的研究及实际设计中粘结强度的计算提供参考.     

积极发挥专业图书馆读者的作用

文章着重从信息采访、信息服务、信息咨询三个方面论述专业图书馆读者在图书馆服务中的作用.     

Revisit the K-segment of the Southeast Indian Ridge for new evidence of hydrothermal plumes

During the first leg of a Chinese research cruise to the Southeast Indian Ridge (SEIR) in 2007, we revisited the K-segment of the SEIR and observed a water column turbidity anomaly at a CTD cast station. This station was located ~400 m from a dredge site on a plume anomaly detected in 1996 by US scientists. The characteristics of the turbidity anomaly detected in our survey are different from those observed 11 years previously, implying that either the detected anomalies have distinct and different plume sources or the robustness of the plume has changed in the intervening time. In addition, turbidity anomalies were observed at two localities on the K-segment in a deep tow-yo profile. If these two anomalies are not connected, the plume incidence calculated would be about 0.28, larger than the predicted value of 0.25 for a 65 mm/a spreading rate. This increase in plume incidence on the K-segment seems to be related to the influence of the St. Paul-Amsterdam hotspot, located about 300 km to the northwest.     

Highlights of recent advances in functional coordination polymers made in China

Coordination polymers (or metal-organic frameworks, MOFs), as one of the essences of crystal engineering and coordination chemistry, have attracted considerable atten-tion from chemists,     

A venomous arthropod in the Early Cambrian Sea

A number of recently evolved animals possess poison glands for feeding and/or defense.However,examples of such animals are rare in the fossil record.We report a fossil arthropod Isoxys curvirostratus from the Early Cambrian Chengjiang biota of China.This species is regarded as the oldest known venomous arthropod based on the presence of venomous glands in its head region.The adult animal is 2-5 cm long and the body is covered entirely with a carapace.The presence of large stalk eyes and a pair of stout grasping appendages with a terminal spine suggest it was raptorial.Interestingly,the two pear-shaped,three-dimensionally preserved objects that are present in the head region and at the base of the grasping appendages closely resemble the venom glands of some living arthropods in size,shape,and position.These features indicate that the presence of venomous predators could date back 520 million years.Furthermore,our observations suggest that the feeding strategies and organs adapted for this purpose had already reached a high level of diversity and anatomical sophistication in the Early Cambrian ecosystems.     

Normalized transfer function for characterizing conversion efficiency and harmonic distortion of all-optical sampling

A novel method for characterizing the nonlinearity of all-optical sampling is proposed based on the normalized transfer function.Simulation results demonstrate the effectiveness of our method.Furthermore,an all-optical sampling experiment is performed to verify our method.Both simulation and experiment show consistency between our method and the measurements.The method only requires normalization and polynomial fitting of the transfer curve,and enables direct expression of the nonlinearity with the coefficients of the normalized transfer function.     

大学生幸福智力量表的适应性检验

运用大学生幸福智力(well-being intelligence,WBI)量表,对132名成人和121名高中生进行测查,结果表明:(1)在成人样本和高中生样本中,量表总的α系数及4个分量表的α系数均高于0.848 8;(2)CFA分析显示拟合指数x2/df、GFI、AGFI等在这2个群体中均比较理想;(3)在这2个群...