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91.
Myopia and ambient lighting at night.   总被引:9,自引:0,他引:9  
G E Quinn  C H Shin  M G Maguire  R A Stone 《Nature》1999,399(6732):113-114
  相似文献   
92.
自从松萎蔫病于1988年首次报道以来,该病已经扩散到韩国大部分地区并引起未能预见的危害。从2000年该病危害急剧增加,到2006年危害面积已达7 871 hm2。2000—2008年伐除的病树已达1 851 225株,年经济损失达1 000万美元。2006年后危害面积下降,2008年下降到6 015 hm2。但至2008年该病已经扩散至韩国54个地区、县和11省。调查发现松材线虫可以被云杉花墨天牛(Monochamus saltuarius)所携带。防治的策略为:(1)连续地监测;(2)病株的早期检测;(3)伐除病株;(4)焚烧和熏蒸伐除的病木。对人文和旅游景点及成熟林可以采取注射法防治,也可采取飞防的方法防治天牛。韩国已经通过了法律,禁止病木的运输。在首次发现该病后连续3 a未发生重复感染的地区,建立无病区和优选系统。  相似文献   
93.
94.
Sil AK  Maeda S  Sano Y  Roop DR  Karin M 《Nature》2004,428(6983):660-664
IkappaB kinase-alpha (IKK-alpha) exhibits protein-kinase-dependent and -independent functions. Its kinase activity is required for lymphoid organogenesis and mammary gland development, whereas a kinase-independent activity is required for epidermal keratinocyte differentiation. In addition to failed epidermal differentiation, IKK-alpha-deficient mice exhibit abnormal skeletal and craniofacial morphogenesis. As similar defects are not exhibited by mice that experience systemic inhibition of NF-kappaB, we postulated that the morphogenetic defects in IKK-alpha-deficient mice are not caused by reduced NF-kappaB activity but instead are due to failed epidermal differentiation that disrupts proper epidermal-mesodermal interactions. We tested this hypothesis by introducing an epidermal-specific Ikka (also known as Chuk) transgene into IKK-alpha-deficient mice. Mice lacking IKK-alpha in all cell types including bone and cartilage, but not in basal epidermal keratinocytes, exhibit normal epidermal differentiation and skeletal morphology. Thus, epidermal differentiation is required for proper morphogenesis of mesodermally derived skeletal elements. One way by which IKK-alpha controls skeletal and craniofacial morphogenesis is by repressing expression of fibroblast growth factor (FGF) family members, such as FGF8, whose expression is specifically elevated in the limb bud ectoderm of IKK-alpha-deficient mice.  相似文献   
95.
Shin C  Feng Y  Manley JL 《Nature》2004,427(6974):553-558
The cellular response to stresses such as heat shock involves changes in gene expression. It is well known that the splicing of messenger RNA precursors is generally repressed on heat shock, but the factors responsible have not been identified. SRp38 is an SR protein splicing factor that functions as a general repressor of splicing. It is activated by dephosphorylation and required for splicing repression in M-phase cells. Here we show that SRp38 is also dephosphorylated on heat shock and that this dephosphorylation correlates with splicing inhibition. Notably, depletion of SRp38 from heat-shocked cell extracts derepresses splicing, and adding back dephosphorylated SRp38 specifically restores inhibition. We further show that dephosphorylated SRp38 interacts with a U1 small nuclear ribonucleoprotein particle (snRNP) protein, and that this interaction interferes with 5'-splice-site recognition by the U1 snRNP. Finally, SRp38-deficient DT40 cells show an altered cell-cycle profile consistent with a mitotic defect; they are also temperature sensitive and defective in recovery after heat shock. SRp38 thus plays a crucial role in cell survival under stress conditions by inhibiting the splicing machinery.  相似文献   
96.
Hsu LC  Park JM  Zhang K  Luo JL  Maeda S  Kaufman RJ  Eckmann L  Guiney DG  Karin M 《Nature》2004,428(6980):341-345
Macrophages are pivotal constituents of the innate immune system, vital for recognition and elimination of microbial pathogens. Macrophages use Toll-like receptors (TLRs) to detect pathogen-associated molecular patterns--including bacterial cell wall components, such as lipopolysaccharide or lipoteichoic acid, and viral nucleic acids, such as double-stranded (ds)RNA--and in turn activate effector functions, including anti-apoptotic signalling pathways. Certain pathogens, however, such as Salmonella spp., Shigellae spp. and Yersiniae spp., use specialized virulence factors to overcome these protective responses and induce macrophage apoptosis. We found that the anthrax bacterium, Bacillus anthracis, selectively induces apoptosis of activated macrophages through its lethal toxin, which prevents activation of the anti-apoptotic p38 mitogen-activated protein kinase. We now demonstrate that macrophage apoptosis by three different bacterial pathogens depends on activation of TLR4. Dissection of anti- and pro-apoptotic signalling events triggered by TLR4 identified the dsRNA responsive protein kinase PKR as a critical mediator of pathogen-induced macrophage apoptosis. The pro-apoptotic actions of PKR are mediated both through inhibition of protein synthesis and activation of interferon response factor 3.  相似文献   
97.
98.
Mutant dynactin in motor neuron disease   总被引:24,自引:0,他引:24  
Impaired axonal transport in motor neurons has been proposed as a mechanism for neuronal degeneration in motor neuron disease. Here we show linkage of a lower motor neuron disease to a region of 4 Mb at chromosome 2p13. Mutation analysis of a gene in this interval that encodes the largest subunit of the axonal transport protein dynactin showed a single base-pair change resulting in an amino-acid substitution that is predicted to distort the folding of dynactin's microtubule-binding domain. Binding assays show decreased binding of the mutant protein to microtubules. Our results show that dysfunction of dynactin-mediated transport can lead to human motor neuron disease.  相似文献   
99.
Kim J  Chung YD  Park DY  Choi S  Shin DW  Soh H  Lee HW  Son W  Yim J  Park CS  Kernan MJ  Kim C 《Nature》2003,424(6944):81-84
The many types of insect ear share a common sensory element, the chordotonal organ, in which sound-induced antennal or tympanal vibrations are transmitted to ciliated sensory neurons and transduced to receptor potentials. However, the molecular identity of the transducing ion channels in chordotonal neurons, or in any auditory system, is still unknown. Drosophila that are mutant for NOMPC, a transient receptor potential (TRP) superfamily ion channel, lack receptor potentials and currents in tactile bristles but retain most of the antennal sound-evoked response, suggesting that a different channel is the primary transducer in chordotonal organs. Here we describe the Drosophila Nanchung (Nan) protein, an ion channel subunit similar to vanilloid-receptor-related (TRPV) channels of the TRP superfamily. Nan mediates hypo-osmotically activated calcium influx and cation currents in cultured cells. It is expressed in vivo exclusively in chordotonal neurons and is localized to their sensory cilia. Antennal sound-evoked potentials are completely absent in mutants lacking Nan, showing that it is an essential component of the chordotonal mechanotransducer.  相似文献   
100.
以相转移催化法合成了单体二烯丙基双酚S醚(DABSE),采用FTIR,1H-NMR对单体结构进行了验证。DSC结果表明:DABSE的熔点为133℃,热聚合温度高达280℃,并具有较宽的聚合峰。  相似文献   
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