排序方式: 共有39条查询结果,搜索用时 15 毫秒
21.
运用微分方程理论,研究了一类非线性的红松种群征税模型,得到了该系统的平衡点,证明了该系统正平衡点的局部渐进稳定性和正平衡点的全局吸引域,利用 Pontryagin 最大值原理给出了最优征税策略。 相似文献
22.
凤尾菇线粒体DNA片段的克隆与同源性分析 总被引:1,自引:0,他引:1
将Sau3AI部分酶切的凤尾菇线粒体DNA插入质粒载体的pBS^+的BamHI位点,然后以凤尾基线粒体DNA为探针与重组质粒DNA进行斑点杂交,筛选到6个阳性克隆,选取其中2个阳性克隆的插入片段分别与来6个属的13个食用菌品种的线粒体DNA进行Southern杂交,其结果显示两探针能与所有测定的侧耳属品种的线粒体DNA杂交,并具多态性,但与其它属线粒体DNA只有不同程度的杂交,两探针中H4的杂交范 相似文献
23.
利用作者克隆的酿酒酵母基因启动子片段Y8为探针和菌落原位杂交方法,从构建的S.cerevisiae YNN27基因组文库中共筛选8个阳性克隆。根据这些克隆中插入片段大小和限制酶切结果,可将其划分为6种,分别命名为YN1-YN6.Southern杂交结果表明,这6种DNA片段不仅能与6.5kb的全长Y8探针我,而且均能与PstI酶切Y8所得的3段DNA探针杂交。 相似文献
24.
RAPD analysis of genetic variability among Stylosanthes guianenesis accessions of resistant and susceptible to the stylo anthracnose 总被引:1,自引:0,他引:1
JiangChangshun ZouDongmei ZhangYizheng 《高技术通讯(英文版)》2005,11(1):85-90
Stylosanthes guianenesis Sw. is an important tropical forage legume grown and utilized in the tropics and the subtropics of South China. Anthracnose, caused by Colletotrichum gloeosporioides (Penz.) Sacc., is a major constraint to the extensive use of Stylosanthes. Forty-five accessions of S. guianensis were assessed with RAPD for genetic diversity and for resistance to anthracnose. RAPD analysis was performed using twenty primers screened from 200 arbitrary oligonucleotides, and a 71.5% level of polymorphism was found. The dendrogram obtained with unweighted pair group method of averages (UPGMA) based on the RAPD data showed genetic similarity from 50% to 94% among all stylo accessions, and fourteen clusters were defined at 66.5% genetic similarity. Two strains of C. gloeosporioides from stylo in China were used for anthracnose resistance screening. All plant accessions showed variation in the reaction to two strains and the correlation of resistance had a value of 0.904. Multiple correspondence analysis displayed a random distribution of the resistance or susceptibility response with respect to the genetic diversity measured by RAPD analysis except one group. Mean distance was also calculated to determine the diversity within clusters. From our results, the RAPD analysis is an effective and efficient technique of providing quantitative estimates of genetic similarity among stylo accessions. 相似文献
25.
李贻铮 《华侨大学学报(自然科学版)》1989,10(3):310-314
本文分析了滑动速度、正压力、润滑油牌号等对黄铜在润滑状态下摩擦系数的影响,指出目前常用设计手册所推荐的摩擦系数的局限性,认为组成运动副的零件的材料不宜都采用黄铜,以及在回转运动副中黄铜不宜作为被包容件的材料。 相似文献
26.
在速率常数的测定中长期存在参数A∞的测量问题,本文拟在介绍一种新方法,该法适用于二级动力学反应,可以提供解决以上问题的可靠方法。对于终点数据无法获得或虽可获得但不可靠时该法特别有用。 相似文献
27.
用体内自发突变,卡那霉素抗性筛选和体外限制酶切富集方法消除载体pNG35中新霉素磷酸转移酶基因内的限制酶位点HindⅢ,除去HindⅢ位点后的载体pNG35△H赋予宿主细胞的卡那霉素抗性水平远远高于原载体pNG35。 相似文献
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29.
油菜单株总DNA的快速制备 总被引:11,自引:5,他引:6
通过改进匀浆过程确定了适合同时提取多个100mg左右植物材料总DNA的实验程序,制备的总DNA经测定紫外吸收曲线,限制酶作用和RAPD扩增,证明完全符合分子生物学实验的基本要求。 相似文献
30.
Feng Zongyun 《高技术通讯(英文版)》2005,11(3):320-324
106 aeeessions of Tibetan wild barley, including 50 accessions of the two-rowed wiht barley Hordeum vulgare ssp. sopntaneum (HS), 27 accessions of the six-rowed bottle-shaped wild barley H. lagunculiforme (HL) and 29 accessions of the six-rowed wiht barley H. agriocrithon (HA) that separately represent different agrigeographical regions of Tibet, were used to study the genetic diversity and genetie differentiation using SSR markers selected from seven barley linkage groups. 229 allelic variants were identified with an average of 7.6 alleles/locus. The average of total number of alleles per locus in HA (6.4) is much higher than that in HS (3.9) and HL (3.4). The genetie diversity and its standard deviation among the three subspecies were in the order of HS〉HL〉HA. Very significant genetic differentiation was observed among the three subspecies of wild barley. Comparisons of the results fiom this and previous studies showed a strong Oriental-Occidental differentiation of barley, and that Shannan region of Tibet might be the center of origin of the Tibetan two-rowed wild barley, thus supporting not only the hypothesis of a mono-phyletie origin of cultivated barley but also the proposition that the Tibetan two-rowed wild barley as ultimate progenitor of Chinese cultivated barley. 相似文献