Cloned pigs produced by nuclear transfer from adult somatic cells

Since the first report of live mammals produced by nuclear transfer from a cultured differentiated cell population in 1995 (ref. 1), successful development has been obtained in sheep, cattle, mice and goats using a variety of somatic cell types as nuclear donors. The methodology used for embryo reconstruction in each of these species is essentially similar: diploid donor nuclei have been transplanted into enucleated MII oocytes that are activated on, or after transfer. In sheep and goat pre-activated oocytes have also proved successful as cytoplast recipients. The reconstructed embryos are then cultured and selected embryos transferred to surrogate recipients for development to term. In pigs, nuclear transfer has been significantly less successful; a single piglet was reported after transfer of a blastomere nucleus from a four-cell embryo to an enucleated oocyte; however, no live offspring were obtained in studies using somatic cells such as diploid or mitotic fetal fibroblasts as nuclear donors. The development of embryos reconstructed by nuclear transfer is dependent upon a range of factors. Here we investigate some of these factors and report the successful production of cloned piglets from a cultured adult somatic cell population using a new nuclear transfer procedure.     

楼板裂缝问题浅析

分析楼屋面板开裂的多种原因,引进收缩梯度概念,衡量在正常配筋情况下楼板两个方面出现裂缝可能性的相对大小,应用于工程实例计算,分析结果与实际情况相符,最后提出预防楼板出现裂缝的设计要点和施工注意事项。     

非同步交流采样误差分析及滤波器设计

导出了工频电参数测量中非同步采样时电压、电流和功率的准确误差公式,设计了侈通滤波器,从而降低非同步引起的误差。仿真计算表明,上述措施计算简单,有效地提高了测量精度。     

书法教育的功能说

书法是中华民族文化的结晶和象征,荟萃了中华民族文化的精髓,有着博大精深的文化内涵和巨大的文化魅力,书法教育是促进素质教育的必然要求和重要手段,书法教育在提高学生文化素质、智能素质、审美素质以及强化人格力量诸方面具有其它学科无法替代的功能。     

贝叶斯规整化神经网络预测苯取代物的毒性

利用贝叶斯规整化神经网络(BRNN)研究了59种苯取代的环境毒性和分子结构的关系.建立了基于脂水分配系数和4种量子指数的QSAR环境毒性预测模型.结果表明:该模型的预测性能超过偏最小二乘法(PLS)和逐步线性回归方法,残差分析显示其稳定可靠,有望成为一种良好的苯取代物毒性的环境评价和预测的方法.     

Ecosystem vulnerability of China under B2 climate scenario in the 21st century

This paper applies climate change scenarios in China based on the SRES assumptions with the help of RCMs projections by PRECIS （providing regional climates for impacts studies） system introduced to China from.the Hadley Centre for Climate Prediction and Research at a high-resolution （50 kmx50 km） over China. This research focuses on B2 scenario of SRES. A biogeochemical model ＂Atmosphere Vegetation Integrated Model （AVIM2）＂ was applied to simulating ecosystem status in the 21st century. Then vulnerability of ecosystems was assessed based on a set of index of mainly net primary production （NPP） of vegetation. Results show that climate change would affect ecosystem of China severely and there would be a worse trend with the lapse of time. The regions where having vulnerable ecological background would have heavier impacts while some regions with better ecological background would also receive serious impacts. Extreme climate even would bring about worse impact on the ecosystems. Open shrub and desert steppe would be the two most affected types. When the extreme events happen, vulnerable ecosystem would extend to part of defoliate broad-leaved forest, woody grassland and evergreen conifer forest. Climate change would not always be negative. It could be of some benefit to cold region during the near-term. However, in view of mid-term to long-term negative impact on ecosystem vulnerability would be enormously.     

绿色技术创新政策的国际经验对福建的借鉴与启示

绿色技术创新是福建可持续发展的重要途径。本文首先对美国、日本和欧盟的绿色技术创新的法律政策、财政税收支持政策、绿色采购政策和教育政策等四个方面进行深入分析,并与福建进行对比分析,找出目前福建在绿色技术创新政策的不足与发展方向,希望对福建省制订和完善绿色技术创新政府以及更好地推进资源节约型和环境最好型社会的建设具有借鉴意义。     

CSP热连轧再结晶临界应变模型研究

通过THERMOMASTER-Z型热／力模拟试验机对CSP生产线上冷轧冲压基板（SPHD）进行了热变形实验，建立了该钢种的临界应变模型以及流动应力-应变模型；分析了试验过程中工艺参数对临界应变、流动应力的影响。结果表明，变形温度、变形速率是影响临界应变的主要因素，当热变形达到临界应变时，将发生动态再结晶，同时造成变形抗力的波动。     

UML 2.0通信图的一种测试用例生成方法

提出了一种UML2.0通信图生成测试用例的方法.首先根据通信图构建一棵通信树,利用后序遍历了所构造的树,从通信图中选择条件断言,然后转换条件断言并通过求解函数最小值的方法来生成测试用例.该测试用例满足消息路径覆盖和边界覆盖.