数字参考咨询服务

文章着重论述了数字参考咨询服务中对用户分析的问题,并简要介绍了东莞图书馆参考咨询服务工作开展的情况。     

唐钢一铁炉料性能及合理搭配的研究

试验研究表明，唐钢一铁烧结矿粒度均匀，具有良好的强度和冶金性能；搭配２５－４０％酸性球团的综合炉料，具有较好的熔滴性能，是合理结构。     

西南科技大学办学特色思考

简述了高校办学特色,并对西南科技大学的3个办学特色进行了阐述：即对共建和产学研联合办学的办学体制特色;材料学、控制理论与控制工程、农学学科显示出的优势学科专业特色;“艰苦奋斗、自强不息”的校园文化特色作了阐述.     

Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AAF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8. A mixture of cosmid cHT2-derived subclones of the 3' part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated.     

以设计新理念树“离军高速”典型示范形象

结合交通部公路工程勘察设计典型示范工程要点,对青岛至银川国道主干线山西省离石至军渡段高速公路从安全、环保、和谐等角度对全新设计理念、重点工程及对策进行了介绍,以达到树立典型示范形象的目的。     

Preferential hydrophobic interactions are responsible for a preference of D-amino acids in the aminoacylation of 5'-AMP with hydrophobic amino acids.

We have studied the chemistry of aminoacyl AMP to model reactions at the 3' terminus of aminoacyl tRNA for the purpose of understanding the origin of protein synthesis. The present studies relate to the D, L preference in the esterification of 5'-AMP. All N-acetyl amino acids we studied showed faster reaction of the D-isomer, with a generally decreasing preference for D-isomer as the hydrophobicity of the amino acid decreased. The beta-branched amino acids, Ile and Val, showed an extreme preference for D-isomer. Ac-Leu, the gamma-branched amino acid, showed a slightly low D/L ratio relative to its hydrophobicity. The molecular basis for these preferences for D-isomer is understandable in the light of our previous studies and seems to be due to preferential hydrophobic interaction of the D-isomer with adenine. The preference for hydrophobic D-amino acids can be decreased by addition of an organic solvent to the reaction medium. Conversely, peptidylation with Ac-PhePhe shows a preference for the LL isomer over the DD isomer.