Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain

Gelsolin is representative of a class of actin-modulating proteins found in lower eukaryotes to mammals, which sever actin filaments. Gelsolin found in the cytoplasm of cells is functionally similar to a mammalian plasma protein of similar size, originally called ADF or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25-amino-acid residue extension on plasma gelsolin which appears to account for the difference in relative molecular mass (Mr) between the proteins as assessed by SDS-polyacrylamide gel electrophoresis (PAGE), 93,000 (93K) and 90K, respectively. Here we report the isolation of full-length human plasma gelsolin complementary DNA clones from a HepG2 library. The inferred amino-acid sequence reveals the presence of a signal peptide, a long tandem repeat that matches the actin-binding domains of gelsolin, a tetrapeptide present in actin and extended regions of identical sequence with rabbit macrophage gelsolin. Southern blot analysis indicates that a single gene in the haploid genome encodes both protein forms.     

起重机卷筒的局部变形分析

本文根据起重机卷筒的受力特点．提出了更加精确的简化荷载，并考虑了简壁与侧板联结处的局部变形，用内力分配法推导出了更精确的强度计算公式。它对卷筒设计具有指导价值。     

Electrochromic effect in domain-inversion process in LiNbO3： Ru： Fe crystals

A reversible electrochromic effect accompanying domain-inversion during the electrical poling process in LiNbO3： Ru： Fe crystals at room temperature has been observed. In electrode area, both electrochromism and domain-inversion occur alternately, and electrochromism is also reversible during back-switch poling, which is experimentally verified and whose mechanism is briefly explained using a microstrncture ferroelectric model. In addition, because of the enhancing elcctrochromic effect, different from the undoped LiNbO3 crystals, the coercive filed （21.0 kV/mm or so） measured in LiNbO3： Ru： Fe is lower than its breakdown field, thus providing a possible new technique for realizing the domain-inversion by constant electric field rather than a pulsed one.     

代数结构的伴随函数研究

在讨论代数结构抽象性的基础上引入了反映代数结构的类数(随基数)变化情况的伴随函数的概念;然后主要利用对合自同构和直积,从给定结构之内、外两个方面的变换出发,给出了构造具有相同伴随函数的代数结构的两种方法;并指出,伴随函数的概念有助于拓广人们对代数结构的认识;文末一般地提出了代数结构函数论的研究问题。     

论企业家能量的四大影响因素

作者提出企业家的能量关系式Ｅ＝Ｍ２Ｉ２，并从企业的人才结构及其作用的发挥，资金结构及其作用的发挥，信息结构及其作用的发挥，以及企业家所具备的首创精神及其作用的发挥这四大方面论述企业家的能量与企业成败的关系。针对目前我国企业在四个方面普遍存在的问题，提出了相应的对策．     

油浸电力设备故障诊断中高分子膜的油气分离研究

进一步探讨了高分子膜油气分离的机理，阐述了高分子膜油气分离的基本理论并进行了详尽的试验分析，论述了在油浸电力设备的故障诊断中利用高分子膜实行油气分离方法的有效性。     

Topological domains in mammalian genomes identified by analysis of chromatin interactions

The spatial organization of the genome is intimately linked to its biological function, yet our understanding of higher order genomic structure is coarse, fragmented and incomplete. In the nucleus of eukaryotic cells, interphase chromosomes occupy distinct chromosome territories, and numerous models have been proposed for how chromosomes fold within chromosome territories. These models, however, provide only few mechanistic details about the relationship between higher order chromatin structure and genome function. Recent advances in genomic technologies have led to rapid advances in the study of three-dimensional genome organization. In particular, Hi-C has been introduced as a method for identifying higher order chromatin interactions genome wide. Here we investigate the three-dimensional organization of the human and mouse genomes in embryonic stem cells and terminally differentiated cell types at unprecedented resolution. We identify large, megabase-sized local chromatin interaction domains, which we term 'topological domains', as a pervasive structural feature of the genome organization. These domains correlate with regions of the genome that constrain the spread of heterochromatin. The domains are stable across different cell types and highly conserved across species, indicating that topological domains are an inherent property of mammalian genomes. Finally, we find that the boundaries of topological domains are enriched for the insulator binding protein CTCF, housekeeping genes, transfer RNAs and short interspersed element (SINE) retrotransposons, indicating that these factors may have a role in establishing the topological domain structure of the genome.     

一种基于网络编码的无线广播高效重传方法

为了提高无线网络中重传数据包的重传效率,提出一种基于异或网络编码的数据包重传方法,并采用了优化的丢失包编码组合策略.     

沉默白细胞介素10基因对猪繁殖与呼吸综合征病毒（PRRSV）感染的影响

为了研究藏猪免疫细胞中IL-10基因表达抑制后对RNA病毒感染及其免疫应答的影响,本实验构建和筛选了抑制猪IL-10基因表达的shRNA干扰分子及其表达载体,并以新型的壳聚糖接枝聚乙二醇和聚乙烯亚胺修饰分子(CS-N-PEI-PEG)通过离子交联法进行分子包装,制备DNA-壳聚糖纳米分子,体外转染藏猪免疫细胞,观察猪繁殖和呼吸障碍综合征病毒(PRRSV)诱导免疫细胞IL-10基因的表达及其增殖感染水平.实验结果表明:与未转染纳米分子或者纳米分子不表达shRNA的空白对照组相比较,shRNA实验组的IL-10基因表达在24～72h内明显抑制(P<0.05),免疫细胞中PRRSV的增殖水平显著下降(P<0.05);同时IL-2、IL-4、IL-6和IFN-γ基因的mRNA水平明显升高(P<0.05).这些提示IL-10的shRNA转染能使免疫细胞的抗感染能力显著增强,甚至可完全抑制PRRSV的复制.     

基于强度调制和相位调制的ROF系统比较

基于现有实现载波抑制毫米波传输下行链路信号的实验,研究并比较了以下两种ROF系统：一种是利用强度调制技术产生载波抑制毫米波的传输系统,另一种是利用相位调制技术产生边带传输下行链路信号．通过仿真实验证明了系统的有效性和可靠性,并根据光谱图、Q值参数、眼图和误码率曲线来对比研究两者传输性能的优缺点．