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301.
WANGZhen MENGXianmin CAOHuiqing LIUDongqing FENGYan YOUXin WANGYin GUOLianjun QUShen 《科学通报(英文版)》2004,49(23):2487-2490
The interaction of nelin, a cardiac-specific expressed protein of human novel gene nelin, with F-actin was studied by both F-actin cosedimentation in vitro and colocalization assays. The results showed that nelin is a new F-actin binding protein and is colocolized with F-actin in cytoplasm of cells. Three new nelin binding proteins, filamin C subtype, titin N2B subtype and inter-alpha trypsin inhibitor heavy chain precursor (ITIH), were identified from human heart cDNA library using yeast two-hybrid screening. The binding activity of filamin C with nelin was confirmed by coimmunoprecipitation. Filamin C binds to nelin through its C-terminal region. It is indicated that nelin is a cytoskeleton associated protein and acts as a membrane-cytoskeleton associated protein involved in the formation of focal adhesions. 相似文献
302.
Persistent organic pollutants PCNs and PBDEs in sediments from coastal waters of Qingdao,Shandong Peninsula 总被引:4,自引:0,他引:4
YANGYongliang PANJing LIYue YINXiaocai SHILei 《科学通报(英文版)》2004,49(1):98-106
Thirty-one congeners of polychlorinated naphthalenes (PCNs) and 21 congeners of polybrominated diphenylethers(PBDEs)in surface sediments at 5 stations and one mussel sample from Qingdao coastal waters were analyzed. The maximum contents occur at the station near the Haibo River mouth. The total PCNs contents are in the range of 212-1209 pg/g dw, dominated by tri-Cl CN. The total PBDEs contents are in the range of 117-5510 pg/g dw,dominated by tri- to hexa-BrBDEs. The local sources of PCNs are likely from combustion processes such as incinerations and coal burnings. The sources of PBDEs are molecular diffusion from the materials containing them. Except for the area near the river mouth where is affected by the sewage sludges, an important source of PCNs and PBDEs in this area is believed to be the atmospheric deposition. Mussels enrich PCNs and PBDEs relative to the sediments. The total TEQs of PCNs in mussels are lower than the total TEQs of PCBs. 相似文献
303.
Abnormal cerebellar development and axonal decussation due to mutations in AHI1 in Joubert syndrome 总被引:11,自引:0,他引:11
Ferland RJ Eyaid W Collura RV Tully LD Hill RS Al-Nouri D Al-Rumayyan A Topcu M Gascon G Bodell A Shugart YY Ruvolo M Walsh CA 《Nature genetics》2004,36(9):1008-1013
Joubert syndrome is a congenital brain malformation of the cerebellar vermis and brainstem with abnormalities of axonal decussation (crossing in the brain) affecting the corticospinal tract and superior cerebellar peduncles. Individuals with Joubert syndrome have motor and behavioral abnormalities, including an inability to walk due to severe clumsiness and 'mirror' movements, and cognitive and behavioral disturbances. Here we identified a locus associated with Joubert syndrome, JBTS3, on chromosome 6q23.2-q23.3 and found three deleterious mutations in AHI1, the first gene to be associated with Joubert syndrome. AHI1 is most highly expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Comparative genetic analysis of AHI1 indicates that it has undergone positive evolutionary selection along the human lineage. Therefore, changes in AHI1 may have been important in the evolution of human-specific motor behaviors. 相似文献
304.
Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV 总被引:1,自引:0,他引:1
JINNingyi ZHANGHongyong YINGefeng ZHENGMin LIUTong JIANGWenzheng LIZijian 《科学通报(英文版)》2004,49(8):823-823
Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV. 相似文献
305.
WUShi-jing GONGZhi-bo YINYong HUANGHe-chao LOUWei-hua 《武汉大学学报:自然科学英文版》2004,9(5):839-844
Making use of Microsoft Visual Studio. NET platform, hierarchical network planning is realized in working procedure time-optimization of the construction by TBM. and hierarchical network graph of the construction by TBM is drawn based on browser. Then the theory of system realization is discussed, six components of system that can be reused are explained emphatically. The realization of hierarchical network panning in Internet provides available guarantee for controlling rate of progress in large-scale or middle-sized projects. 相似文献
306.
This research examined the typical flavor compounds in the commonest type of Khat called Harari Khat grown in the region of Ethiopia.Twenty-eight compounds, which includes 1,2-Propanedione,1-Phenyl,Hexanol,Hexanal compounds,Limonene, Benzaldehyde with other flavors, were extracted by polydimethylsiloxane at room temperature for 30min from Khat samples,and identified by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS).This method needs no organic solvents and required minimal sample. 相似文献
307.
308.
中国古典文学作品中众多的女性形象丰富了文学的世界,但是受以男权为中心的社会格局的影响,大多数文学作品对于女性的描摹很简略,这和女性在历史发展中所起的作用是不相符的。《史记·项羽本纪》中的虞姬仅在项羽兵败时才出现,所占笔墨极少,但却给后世的文人及艺术以无限的想象空间。本文试图从虞姬短暂的形象展示和《虞美人》词所展现的艺术世界来解读虞姬们的悲剧——其实也是“人”的悲剧。 相似文献
309.
利用^15N稳定性同位素进行水稻、烤烟的盆栽与田间试验,结果表明,贵州黄壤的供氮能力可以用“A”值表示,不同海拔高度的土壤养分校正系数在0.2-0.3之间。 相似文献
310.