Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder

Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Here we sequenced the exomes of nine individuals with TCC and screened all the somatically mutated genes in a prevalence set of 88 additional individuals with TCC with different tumor stages and grades. In our study, we discovered a variety of genes previously unknown to be mutated in TCC. Notably, we identified genetic aberrations of the chromatin remodeling genes (UTX, MLL-MLL3, CREBBP-EP300, NCOR1, ARID1A and CHD6) in 59% of our 97 subjects with TCC. Of these genes, we showed UTX to be altered substantially more frequently in tumors of low stages and grades, highlighting its potential role in the classification and diagnosis of bladder cancer. Our results provide an overview of the genetic basis of TCC and suggest that aberration of chromatin regulation might be a hallmark of bladder cancer.     

Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation

Canalization, also known as developmental robustness, describes an organism's ability to produce the same phenotype despite genotypic variations and environmental influences. In Drosophila, Hsp90, the trithorax-group proteins and transposon silencing have been previously implicated in canalization. Despite this, the molecular mechanism underlying canalization remains elusive. Here using a Drosophila eye-outgrowth assay sensitized by the dominant Kr(irregular facets-1)(Kr(If-1)) allele, we show that the Piwi-interacting RNA (piRNA) pathway, but not the short interfering RNA or micro RNA pathway, is involved in canalization. Furthermore, we isolated a protein complex composed of Hsp90, Piwi and Hop, the Hsp70/Hsp90 organizing protein homolog, and we demonstrated the function of this complex in canalization. Our data indicate that Hsp90 and Hop regulate the piRNA pathway through Piwi to mediate canalization. Moreover, they point to epigenetic silencing of the expression of existing genetic variants and the suppression of transposon-induced new genetic variation as two major mechanisms underlying piRNA pathway-mediated canalization.     

嵌入式系统上的图形界面输入引擎的研究

嵌入式系统上的图形用户界面（GUI）由于基于不同的软硬件平台,输入设备有很大差别,所以开发者在移植时要为不同的输入设备开发其与GUI之间的接口,以使得GUI能够正确响应用户的输入。GUI的输入引擎就是介于GUI处理核心和操作系统的输入设备驱动程序之间的一组抽象接口,它的功能是使不同的输入设备的驱动程序能够无缝地连接到GUI的消息处理程序中。因此,输入引擎可以改善GUI的可移植性。在基于at91rm9200微处理器和嵌入式Linux操作系统的软硬件平台上．讨论了开发GUI输入引擎的一般方法,并结合一个具体的GUI系统——MiniGUI,给出了一个输入引擎实例的开发过程。     

发散思维教学与学生创新能力的培养

本文用发散思维方法于无机化学教学过程，从不同的方向，不同的侧面，不同的层次，横向拓展，纵向深入，将学生的思维积极引向探索创新，克服思维定势，激发思维潜能，有助于学生全面把握相关知识点的相互联系和创新能力的提高。     

灰潮土油菜复肥的产量品质效益

速效磷钾含量中上等的灰潮上研究,施用氮磷复肥能增强油菜植株氮磷的吸收能力,促进营养物质积累,增加角果数和粒数,增产极显著;并明显提高油菜籽粒蛋白质及氨基酸含量,降低硫甙含量。在氮磷复肥基础上配合施钾肥则能显著提高产油量,降低芥酸含量。     

关于卡诺循环的定义

本文对卡诺循环定义的提法作出讨论。