一步法合成碳酸二甲酯的研究

采用活性炭负载无机碱金属氢氧化物,制备新型负载催化剂,实现由二氧化碳、甲醇和环氧丙烷一步法合成碳酸二甲酯,并重点研究了反应条件对催化剂性能的影响.结果表明,以活性炭为载体,氢氧化钠为前驱体,负载量15 %,焙烧温度为600 ℃,反应初始压力为4 MPa的反应条件下甲醇和环氧丙烷的转化率分别为28.1 %和99.9 %,碳酸二甲酯(DMC)和丙二醇(PG)的选择性达到最高,分别为39.8%和40.5%.     

采用工艺巷防止高瓦斯易自燃综放面上隅角瓦斯积聚的探讨

上隅角瓦斯积聚是采用综呆放顶煤技术开采高瓦斯易自燃煤层必须面对的难题。文章提出了利用工艺巷防止综放面上隅角瓦斯积聚的方法。并且,根据计算流体力学理论建立数学物理模型,模拟计算了综放工作面及采空区内空气、瓦斯流动的规律,有力地论证了该方法的可行性和有效性,为我国高瓦斯易自燃煤层综放工作面的瓦斯防治工作提供了新的思路。     

The complete genome of an individual by massively parallel DNA sequencing

The association of genetic variation with disease and drug response, and improvements in nucleic acid technologies, have given great optimism for the impact of 'genomic medicine'. However, the formidable size of the diploid human genome, approximately 6 gigabases, has prevented the routine application of sequencing methods to deciphering complete individual human genomes. To realize the full potential of genomics for human health, this limitation must be overcome. Here we report the DNA sequence of a diploid genome of a single individual, James D. Watson, sequenced to 7.4-fold redundancy in two months using massively parallel sequencing in picolitre-size reaction vessels. This sequence was completed in two months at approximately one-hundredth of the cost of traditional capillary electrophoresis methods. Comparison of the sequence to the reference genome led to the identification of 3.3 million single nucleotide polymorphisms, of which 10,654 cause amino-acid substitution within the coding sequence. In addition, we accurately identified small-scale (2-40,000 base pair (bp)) insertion and deletion polymorphism as well as copy number variation resulting in the large-scale gain and loss of chromosomal segments ranging from 26,000 to 1.5 million base pairs. Overall, these results agree well with recent results of sequencing of a single individual by traditional methods. However, in addition to being faster and significantly less expensive, this sequencing technology avoids the arbitrary loss of genomic sequences inherent in random shotgun sequencing by bacterial cloning because it amplifies DNA in a cell-free system. As a result, we further demonstrate the acquisition of novel human sequence, including novel genes not previously identified by traditional genomic sequencing. This is the first genome sequenced by next-generation technologies. Therefore it is a pilot for the future challenges of 'personalized genome sequencing'.     

试析《傲慢与偏见》中的伊丽莎白

简·奥斯丁《傲慢与偏见》中塑造的伊丽莎白·班纳特,经历无数读者和批评家的检验与批评后,仍然深受现代读者的喜爱。在现代社会的审美观下,伊丽莎白的美丽、倔强和聪慧的个性;对梦想的不懈追求和其本身具有的灰姑娘的特质构成了她独有的魅力,使她的形象愈加迷人。     

变截面管道模型的等离子体输运特性数值研究

为了研究强流脉冲离子束(HIPIB)辐照靶材产生烧蚀等离子体向真空中喷发的机制,建立了与HIPIB烧蚀靶材形状相关的沿变截面管道流动的等离子体喷发动力学模型,并以烧蚀结果为初始条件采用数值方法计算了喷发等离子体压力、密度及速度的时空演化规律。结果表明:密度演化结果与一维明显不同,随时间增加峰值先增加而后再减小,且逐渐趋于平缓,存在峰值位置,说明薄膜的形成与基片相对靶材位置是相关的。该结果对HIPIB辐照进行薄膜生长具有一定的参考价值和指导意义。     

试论辛弃疾的经济生活与词创作

辛弃疾的经济生活堪为南宋高级文官的代表。其俸禄收入来源广,数量丰,支持其基本生活所需绰绰有余。其良好的经济状况影响到生活方式,使其广交游、多宴饮,从而催生了词创作活动,导致其偏好词体,具有尊体意识。而辛词所表现的平和心态以及崇雅尚真的审美趣味,也或多或少受其经济生活的影响,并通过词作的题材、风格等方面得以呈现。     

基于WS-Security的SOA安全协议框架设计

面向服务的架构(SOA)具有平台无关性,突破企业内部网的限制,实现供应链上下游伙伴业务的紧密结合等优点.文章根据SOA的架构设计理念,设计了面向web服务的安全协议框架;研究结果较好地解决了系统集成的问题,而且系统间消息安全性也得以保障.     

我国居民消费价格指数灰色关联分析

采用灰色关联分析法,对我国居民消费价格指数进行了多因素关联分析.得出房屋销售价格、商品零售价格以及国内生产总值指数是我国近期CPI过快增长的主要影响因素.对此进行了原因分析并提出相应建议.     

应用胶束电动色谱和模式识别技术溯源茶叶产地的研究

采用胶束电动色谱法(MEKC)建立了2类共11个中国红茶样品的色谱指纹图谱,结合模式识别技术对不同产地来源的红茶加以区分.红茶样品的处理方法:去离子水(100℃)浸取10 min.MEKC色谱条件:缓冲溶液25 mMNa2HPO4-KH2PO4-100mM SDS-0.6%甲醇(V/V)-1 mM EDTA(pH7.0...     

基于HS-SPME/GC-MS与模式识别技术判别红茶产地

本文建立一种基于顶空固相微萃取气质联用法(HS-SPME/GC-MS)分析红茶中挥发性组分,并结合模式识别方法实现了红茶的产地鉴别.采用HS-SPME法在100℃提取60 min,热解吸法直接进样气质联用仪(GC-MS)分析.根据挥发性组分的总离子流图,采用聚类分析、主成分分析和线性判别分析等模式识别技术实现了对不同产地红茶的判别.