TIN2 is a tankyrase 1 PARP modulator in the TRF1 telomere length control complex

Telomere length in humans is partly controlled by a feedback mechanism in which telomere elongation by telomerase is limited by the accumulation of the TRF1 complex at chromosome ends. TRF1 itself can be inhibited by the poly(ADP-ribose) polymerase (PARP) activity of its interacting partner tankyrase 1, which abolishes its DNA binding activity in vitro and removes the TRF1 complex from telomeres in vivo. Here we report that the inhibition of TRF1 by tankyrase is in turn controlled by a second TRF1-interacting factor, TIN2 (ref. 6). Partial knockdown of TIN2 by small hairpin RNA in a telomerase-positive cell line resulted in telomere elongation, which is typical of reduced TRF1 function. Transient inhibition of TIN2 with small interfering RNA led to diminished telomeric TRF1 signals. This effect could be reversed with the PARP inhibitor 3-aminobenzamide and did not occur in cells overexpressing a PARP-dead mutant of tankyrase 1. TIN2 formed a ternary complex with TRF1 and tankyrase 1 and stabilized their interaction, an effect also observed with the PARP-dead mutant of tankyrase 1. In vitro, TIN2 protected TRF1 from poly(ADP-ribosyl)ation by tankyrase 1 without affecting tankyrase 1 automodification. These data identify TIN2 as a PARP modulator in the TRF1 complex and can explain how TIN2 contributes to the regulation of telomere length.     

煤矿排水设备性能的自动检测

本文提出了利用单板计算机对井下排水设备性能进行自动检测的方法,其中包括检测系统、机械量转换、计算机程序及系统误差分析等内容,为解决煤矿固定设备的性能检测提出了新的途径.     

论文学批评在当代文化视野中的位置与作用

当前学批评存在的趋于平庸、品位不高乃至失语失范的滑坡现象，期待着批评本身走向科学化和规范化。在当代化视野的背景上对学批评进行本体追问，阐明批评的一般化存在，并揭示批评本体的主要内蕴关系，将有助于探讨学批评对于当今社会、当代化及公民个人的人意义。     

飞行仿真系统的确认、验证和认证研究

根据飞行仿真系统的实际需求给出了确认、验证和认证(VV&A)的新的定义。应用新的VV&A定义结合全功能飞行仿真系统的特点提出了适用的VV&A过程模型,另外,还提出了主输出带置信度评估方法。上述VV&A的理论与方已成功地在“J5QM-2型飞行仿真系统”和“直升机旋翼自转着陆飞行仿真系统”的置信度评估过程中应用，收到了良好的应用效。     

安全支付网关技术研究与系统实现

安全支付网关是银行与internet之间的安全枢纽 ,是电子商务系统中最重要的组成部分 ,它涉及密码学、信息系统安全和风险管理等学科。在简要介绍电子商务系统安全体系结构的基础上 ,研究了安全支付网关中的安全电子交易 (SET)协议和共钥基础设施PKI等关键技术 ,以及数字签名、数字信封和数字双签名等相关的基础技术 ,并在此基础上完成了“WT0 37安全支付网关系统”的系统实现     

小球在粘滞液体中运动情况的探讨

通过对小球在粘滞液体中运动情况的探讨,为设计制作和改进落球式粘滞系数实验仪提供一定的理论依据.     

1,3,4—恶二唑的酰基硫脲衍生物的合成

从邻氯苯酚出发合成邻氯苯氧乙酰异硫氯氰酸酯，分别与５种２－氨基－５－芳基－１，３，４－恶二反应得到１，３，４－恶二唑的邻氯苯氧乙酰基硫脲衍生物，用ＩＲ，＾１ＨＮＭＲ，元素分析等对它们的结构进行了确证，并分析归纳合成该目标产物的最佳合成条件。     

结构光测量中的定标

分析了ＤＬＴ定标模型的原理和方法，根据所涉及的应用场合和界定的条件，对ＤＬＴ模型进行了简化，由此只需进行二维测量和计算，就能准确有效地完成三维定标．对定标误差进行了理论分析和实验，提出了误差纠正模型及其相应算法，保证了系统的扫描精度．实验表明：提出的定标算法达到像素级精度．     

Low dipole moment large β electrooptic chromophores based on exocyclic double bond conjugated bridge

Novel low dipole moment (μ) and large first hyperpolarizability (β) electrooptic chromophores have been designed based on the special characteristic of reversed dipole moment in the excited state of exocyclic double bond (ECDB) conjugated bridge by the optimization of the substituted method, and their electronic and second-order nonlinear optical properties have been theoretically investigated by employing the AM1/FF and ZINDO/S-CI approaches. By extending the conjugation length and optimizing the donor/acceptor strength, the oscillator strength of the excited transition that contributes to the molecular nonlinearity can be further enhanced. The designed chromophores possess a larger figure of merit (FOM) than that of 4-N,N-dimethylamino-4′-nitrostilbene (DANS), and lower (<5 D) ground state dipole moment.     

竹林的主要病虫害及其防治措施

介绍了竹林常见的病害、虫害及其防治措施。