Study on cellular internalization of poly（vinyldiaminotriazine）-based hydrogen bonding type non-viral transgene vector

Previously we successfully prepared poly（vinyldiaminotriazine）（PVDT）-based non-viral vectors which complexed piasmid DNA via hydrogen bonding with adenine-thymine base pairs, in this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events： the aggregation induced by the instability of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin III were used to inhibit clathrin-and caveolae-mediated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by （i） a more en- ergy dependent process and （ii） a much slow transfection-effective internalization.     

区域科技投入与产出的关系分析

构建科技投入与产出的指标体系,以灰色关联分析为基础,依据河南省2000—2004年的统计数据,分析不同的科技投入对科技产出的影响,从而在河南省未来的科技投入中有的放矢,为政府相关部门决策提供一些有益的建议.     

酶法水解棉籽蛋白的制备与应用

以脱酚后的棉籽粉为原料 ,研究了酶解法制备可溶性棉籽水解蛋白的生产工艺。对其影响因素和产品的实际应用作了探讨。结果表明 ,利用中性蛋白酶AS1.398水解棉籽粉 ,产品得率可达 28.8%,蛋白质量分数为63.28%。棉籽水解蛋白可作为菌体发酵氮源。     

基于RBF网络的增量学习

提出了一种基于径向网络的增量学习算法 ,在对新样本进行学习时 ,通过恢复受干扰样本的方式来尽可能保持已有的知识不变 .模拟实验证明了本算法的正确性和有效性 .     

优先级倒置问题的集成资源管理技术

提出一种集成的实时资源管理和实时服务器技术,它对进程间通信和同步机制进行有效地管理;同时在两种技术的基础之上,构造了一个比较高效的优先级倒置问题的控制算法,克服了传统的用优先级继承方法来解决优先级倒置问题在最坏情况下延迟很大,而用优先级冲顶方法会引起低优先级进程阻塞的缺点。通过这种机制,既可以使系统避免多重优先级倒置现象的发生,又可以使系统的行为具有可分析性和可预测性。     

复杂接触下大理岩球砾碰撞破碎特征

落石是山区常见的地质灾害之一。为研究块石在复杂接触条件下的运动轨迹,利用改进的分离式霍普金森杆系统开展大理岩球砾冲击试验,研究了球砾碰撞破碎特征,探究了冲击角度、接触面粗糙度等因素对试样破碎的临界速度、破碎特征以及尺寸分布的影响。此外,采用高速摄影机观察破碎特征,并将试样碎片进行筛析,分析试样在不同裂纹发育阶段的碰撞破碎特征。试验结果表明：冲击角度45°≤θ≤90°,尖齿角度α=180°,速度一定时：冲击角度θ越大则试样越容易破裂发育裂纹,试样的破坏率随着冲击角度的增大而增大;冲击角度θ=90°,速度一定时：尖齿高度一定时,尖齿角度α越小,试样越容易破裂发育裂纹,试样最终的破坏率随着尖齿角度α的增加而降低;尖齿角度α一定时,尖齿高度越小,试样越容易破裂发育裂纹,试样最终的破坏率随着尖齿高度的增加而降低。因此,增加接触面尖齿角度或减小冲击角度可以有效降低落石冲击时块石的破碎程度。     

IIS控制器的ASIC设计与实现

介绍了IIS控制器的基本原理与实际应用,详细阐述了在专用集成电路中该控制器的设计思路,其中重点介绍了主控模块和收发模块的架构。在用verilog语言实现整个设计的基础上,运用ModelSim进行了功能仿真,利用SMIC0.13μm工艺库和SYNOPSYS的Design Compiler对其综合。经过FPGA验证,确保该控制器作为一个独立的IP核可嵌入到ASIC系统中。     

Micelles-template induced organic nanocrystals based on iodo…nitro interactions

The nanocrystals based on a polar organic compound INBP （4-iodo-4＇-nitrobiphenyl） have been fabricated by reprecipitation approach. For the formation of INBP based nanocrystals, the intermolecular iodo…nitro interaction is the dominant driving force and the micelles formed by the surfactant CTAB （cetyltrimethylammonium bromide） molecules are the efficient templates for controlling morphologies of nanocrystals. The field emission scanning electro microscope （FESEM） images demonstrated that prism-like and needle-like nanocrystals of INBP were formed in pure water and micell containing aqueous solution, respectively. XRD pattern and UV/vis absorption spectra were employed to characterize the nanocrystals.     

高清晰度电视传送复用器实现技术

以高清晰度电视传送复用器的实时实现为目的,在概述ＭＰＥＧ－２传送层语法规范的基础上,给出了传送复用器实现的设计方案和实现技术．传送复用器的功能是将音频、视频及辅助数据按ＭＰＥＧ－２传送层语法规范打成ＴＳ包并送往信道．文中提出的方法是用ＤＳＰ查询复用器各输入缓冲区的状态,根据各缓冲区的状态读入数据,完成音、视频及辅助数据的ＴＳ打包,并保证各ＴＳ包交织均匀,最后给出了实现结果