辣木植物ISSR分子标记的初步研究

对从7个不同地方采集的7份辣木(Moringa)材料进行了ISSR 标记分析.结果表明,被测材料间ISSR标记多态性较高.从18个ISSR 引物中筛选出6个可扩增出清晰的且具多态性的条带的引物,共扩增出75条带,其中59条具有多态性, 占总扩增带数的78.67 %,其中每个引物可扩增出4～14条多态性带,平均10.17条.ISSR 标记遗传相似性系数(GS) 变异范围为0.557 0～0.836 7,平均值为0.687 6±0.080 9.聚类分析表明,7份辣木材料可以聚为四类,其中MS2为单独一类.ISSR 标记可以有效地评价辣木植物遗传分化并为辣木种类的鉴别提供一定的理论依据.     

Non-invasive measurement and validation of tissue oxygen saturation covered with overlying tissues

In this paper, the biological tissue oxygen saturation （rSO2） is obtained non-invasively and in real time based on near infrared spectroscopy （NIRS） using two emitting wavelengths and two detectors, where the tissue is covered with overlying tissues. Our group developed an NIRS oximeter based on the above principle independently, and validated it using liquid tissue model calibrations and animal experiments. The results indicate that （1） in the normal range of tissue oxygen saturation （40-70%）, the rSO2 measured by NIRS is accurate enough and little influenced by the background absorptions （such as the absorption of water） and overlying tissues （such as fat）; （2） during cerebral hypoxia and recovery of three piglets, there is excellent correlation （p 〈 0.001） between cerebral rSO2 and jugular venous oxygen saturation （SjO2）, meaning that the rSO2 can be indicated by the SjO2 to a large extent; during the death of the three piglets induced by heart beat stopping, cerebral rSO2 decreases continuously to significantly low levels （〈25%） because cerebral blood supply does not exist any more. All the above results are of explicit physiological importance.     

Immunorecognition of estrogen and androgen receptors in the brain and thoracic ganglion mass of mud crab, Scylla paramamosain

The brain and the thoracic ganglion of a crustacean can synthesize and secrete gonad-stimulating hormone (GSH) which stimulates the maturation of gonad. In the previous experiments, sex steroid hormones (estradiol, testosterone, progesterone, etc.) have been detected from the crustacean. However, the feedback regulation of sex steroid hormones on the brain and the thoracic ganglion of the crustacean has not been reported so far. In the present experiment, monoclonal antibodies were applied to investigate the immunorecognition of estrogen receptor (ER) and androgen receptor (AR) in the brain and the thoracic ganglion mass of Scylla paramamosain. The results showed that the distribution of the immunopositive substances of ER and AR was extremely similar. They distributed in the protocerebrum, deutocerebrum and tritocerebrum of the brain, and mainly in protocerebrum. In the thoracic ganglion mass, immunopositive substances distributed in the subesophageal ganglion, thoracic ganglion and abdominal ganglion, and mostly in subesophageal ganglion. Immunopositive substances of ER and AR mostly existed in the cytoplasm of neurons. The present study will provide morphological evidence for the origin and the evolution of ER and AR. In addition, the immunoreactivities of ER and AR suggested that the estrogen and androgen may be involved in the feedback regulation of crustacean neuroendocrine.     

咳嗽中体现的数学问题

本文主要讨论了咳嗽中体现出的呼入和呼出的空气速率问题,以及与肺内压力和气管半径相关的流体体积问题．并给出了具体的解答方法．     

区域科技投入与产出的关系分析

构建科技投入与产出的指标体系,以灰色关联分析为基础,依据河南省2000—2004年的统计数据,分析不同的科技投入对科技产出的影响,从而在河南省未来的科技投入中有的放矢,为政府相关部门决策提供一些有益的建议.     

图C_m·K_n的邻强边染色

将顶点集和边集分别为V={v_(ij)┃i=1,2,…,m;j=0,1,…,n-1},E={v_(10)v_(20),v_(20)v(30),…,v_(m0)v_(10)}U(Uim-1)(ij)ik┃j≠k,j,k=0,1,…,n-1}的图简记为Cm·Kn.利用图分解和色集置换的方法,给出了图Cm·Kn的邻强边色数。     

丙酮的气相吸收总传质系数的研究

文章为了研究气相总传质系数的关联式,实验采用安捷伦6820气相色谱仪、清水作为吸收剂,吸收空气——丙酮混合气体中的丙酮;用气相色谱仪测定填料吸收塔进口及出口气体中丙酮的摩尔分率,得出气相总传质系数的关联式。该式不仅简单,使用方便,且实验值与计算值较一致。     

食用菌中高分子活性中心元素分布与其自由基清除能力之间的关系

测定了食用菌的含水量(w_水)、高分子活性中心元素含量以及羟自由基清除能力,计算了食用菌中高分子活性中心元素(Ti、V、Cr、Mn、Mo、Zn、Fe、Co、Ni、Cu和Sr)的分布。结果表明,由含水量校正过的高分子活性中心元素分布参数(1－w_水)^１／３ln(k^２r_１/r_２)与羟自由基清除能力参数ln[F(1－w_水)^３]之间有很好的线性关系。由此笔者认为可以把此高分子活性中心元素分布参数作为近似判断抗氧化能力大小的依据之一,通过此参数可评价食用菌的抗氧化性。     

人工神经网络-近红外光谱法非破坏监控蛇床子SFE-CO2萃取物的质量

将人工神经网络与近红外漫反射光谱相结合, 对蛇床子超临界CO₂萃取物中的蛇床子素和欧前胡素同时进行快速、 非破坏的定量分析. 分别利用 萃取物的原始光谱和两种预处理光谱（一阶导数和标准归一化）建立了网络的数学校正模型, 并把所得结果进行了比较, 确定用一阶导数光谱所建立的模型为最佳网络模型.     

新疆城市化水平区域差异性研究

城市化是经济、社会发展的产物,近年来倍受关注．因而,对城市化发展进行客观、精确、综合的评价就显得十分必要．对1990年～2006年新疆的15个城市的城市化水平作比较,依据结果对这15个城市的城市化水平作了阶段性分析,找出了各阶段的变化特点及发展状况,得出新疆城市化区域差异性．