A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

民营企业家体育锻炼与生活方式及健康现状的调查分析

对浙江沿海3城市(温州市、宁波市、台州市)208名(25岁～59岁)民营企业家的身体健康状况、体育锻炼情况与生活方式等进行调查,结果表明:浙江沿海3城市民营企业家身体健康状况不容乐观.体质测试总体合格率为65.86%,低于全国的合格率(71.40%)和浙江省的平均水平(79.70%).大多数民营企业家日常生活方式差,参加体育锻炼的人数少.建议民营企业家要养成良好的生活习惯,重视卫生保健知识的自我教育,积极参加体育锻炼.     

太中银线吕梁段方案研究

文章以太中银线吕梁段方案为例,分析了影响线路方案的主要因素,强调了线路选线过程中的主要技术标准以及与地方规划协调的重要性。     

HACCP在石油行业野外施工食堂中应用的绩效分析

将HACCP这一世界公认的食品安全控制系统引入野外施工食堂卫生管理当中.方法采用文献检索,事故分析、现场调查和Delphi等研究方法,通过对野外施工食源性疾病危害分析、建立关键控制点,形成野外施工食堂管理HACCP管理规范,并组织实施.结果实施HACCP体系后,从业人员手、餐具、菜墩、抹布的抽查合格率有显著提高,食堂从业人员以及参加施工人员对饮食卫生以及食堂卫生管理知识的掌握显著提高.结论在野外施工食品卫生管理中引入HACCP管理体系,控制食源性疾病取得了良好的绩效.     

湿陷性黄土地区公路边坡变形及滑坡的防治

文章通过分析湿陷性黄土地区公路边坡变形及滑坡的基本类型、形成条件及原因,提出了在该地区防治变形及滑坡的具体措施.     

A Digital Right Management Model for Distribution of Digital Audiovisual Content

0 IntroductionDue to the success of Internet technology and the emer-gence of wide band network,downloading encoded dig-ital audiovisual content files via HTTP,FTP,etc ,had gainedfavor amongInternet users because of thei mmediate access todesired content . Digital Right Management (DRM) system,can satisfy the content provider by encrypting digital audiovi-sual content andli miting access to onlythose people who haveacquired a proper license to play backthe content[1].DRMas defined bythe …     

佛山市城市公共交通发展策略

通过对佛山市公共交通客流和公共交通设施现状的调查，掌握了居民出行规律和客运交通特征指标．采用定性和定量相结合的方法，综合评价了公共交通现状的水平．在分析佛山市未来社会经济、城市发展基本特征的基础上，根据客流分布特点、发展态势及客流交通的生成变化特征，采用经验加模型系统方法，科学地预测发展需求．结合佛山市的实际，分析了公共交通的优劣势，确定了优先发展公共交通的方针与目标．对公共交通政策进行了研究，并提出确保实施公共交通目标的对策和措施     

钢筋混凝土桁架筒的分析与研究

就钢筋混凝土桥架简体系进行了初步的分析与研究，表明这种结构体系有着良好的力学性能、抗震性能，且经济效益显著，有广阔的发展前景。