A novel genome-wide fulllength kinesin prediction analysis reveals additional mammalian kinesins

Kinesins are microtubule-based motor proteins that perform diverse functions[1―6], including the transloca- tion of vesicles, organelles, chromosomes, proteincomplexes, RNA-binding proteins (RNPs), etc. They also help to orchestrate microtubule dynamics …     

液压阀阻力控制分析方法

本文提出的分析方法是建立在阻力控制原理的基础之上，利用液压阀内部各种液阻之间的联接和控制关系，分析液压阀的工作原理和压力流量特性，并能定量计算特性曲线，用以指导液压阀的正确设计。     

QD60PZ型高效低噪声电冰箱压缩机的设计

文章分析了影响压缩机输气量Ｇｓ的诸多因素，提出了半直接进气的改进设计方案．该方案提高了压缩机的效率，但噪声值增大，为进一步控制噪声，将吸气消声器进行适当改进，推出了ＱＤ６０ＰＺ型高效低噪声压缩机，这种设计不仅产生了高效低噪声的效果，而且对压缩机的其它性能均未产生不良影响，达到了国际同类产品的改进设计效果。     

Synthesis and crystal structure of the trimetallic erbium tetrahydride complex [Li(THF)4][{(ButCp)2Er(μ-H)} 3-(μ3-H)]

Reaction of [(Bu′tCp)2Er(μ-CI)]2 with Bu′Li in 1:1 molar ratio in THF at -78℃, after work-up, afforded the trimetallic erbium tetrahydride complex [Li(THF)4][{(Bu′Cp)2Er(μ-H)}3(μ3-H)] (1) by β-hydrogen elimination reaction. Crystal structure determination reveals that complex 1 has discrete ion pair structure. The anion is composed of three (Bu′Cp)2Er units to form a triangle array connected by three bridged hydrogen atoms, and the fourth hydrogen atom coordinated to three Er^3 ions. The coordination num-ber for each Er^3 ion is 9.     

HAb18G/CD147-mediated calcium mobilization and hepatoma metastasis require both C-terminal and N-terminal domains

HAb18G/CD147 is a heavily glycosylated protein containing two immunoglobulin superfamily domains. Our previous studies have indicated that overexpression of HAb18G/CD147 enhances metastatic potentials in human hepatoma cells by disrupting the regulation of store-operated Ca²⁺ entry by nitric oxide (NO)/cGMP. In the present study, we investigated the structure-function of HAb18G/CD147 by transfecting truncated HAb18G/CD147 fragments into human 7721 hepatoma cells. The inhibitory effect of HAb18G/CD147 on 8-bromo-cGMP-regulated thapsigargin-induced Ca²⁺ entry was reversed by the expression of either C or N terminus truncated HAb18G/CD147 in T7721C and T7721N cells, respectively. The potential effect of HAb18G/CD147 on metastatic potentials, both adhesion and invasion capacities, of hepatoma cells was abolished in T7721C cells, but not affected in T7721N cells. Release and activation of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were found to be enhanced by the expression of HAb18G/CD147, and this effect was abolished by both truncations. Thapsigargin significantly enhanced release and activation of MMPs (MMP-2 and MMP-9) in non-transfected 7721 cells, and this effect was negatively regulated by SNAP. However, no effects of thapsigargin or SNAP were observed in T7721 cells, and expression of HAb18G/CD147 enhanced secretion and activation of MMPs at a stable and high level. Taken together, these results suggest that both ectodomain and intracellular domains of HAb18G/CD147 are required to mediate the effect of HAb18G/CD147 on the secretion and activation of MMPs and metastasis-related processes in human hepatoma cells by disrupting the regulation of NO/cGMP-sensitive intracellular Ca²⁺ mobilization although each domain may play different roles.Received 1 April 2004; received after revision 15 June 2004; accepted 22 June 2004     

Abnormal cerebellar development and axonal decussation due to mutations in AHI1 in Joubert syndrome

Joubert syndrome is a congenital brain malformation of the cerebellar vermis and brainstem with abnormalities of axonal decussation (crossing in the brain) affecting the corticospinal tract and superior cerebellar peduncles. Individuals with Joubert syndrome have motor and behavioral abnormalities, including an inability to walk due to severe clumsiness and 'mirror' movements, and cognitive and behavioral disturbances. Here we identified a locus associated with Joubert syndrome, JBTS3, on chromosome 6q23.2-q23.3 and found three deleterious mutations in AHI1, the first gene to be associated with Joubert syndrome. AHI1 is most highly expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Comparative genetic analysis of AHI1 indicates that it has undergone positive evolutionary selection along the human lineage. Therefore, changes in AHI1 may have been important in the evolution of human-specific motor behaviors.     

Research on effects of hydrostatic pressure on the dielectric property of Pb(Zn<Subscript>1/3</Subscript>Nb<Subscript>2/3</Subscript>)O<Subscript>3−</Subscript>BaTiO<Subscript>3−</Subscript> PbTiO<Subscript>3</Subscript> relaxor ferroelectric ceramics

The dielectric properties of Pb(Zn1_1/3Nb_2/3)O₃₋ BaTiO₃₋PbTiO₃ relaxor ferroelectric ceramics near the rhombohedral and tetragonal phase boundary were investigated under hydrostatic pressure. It was found that hydrostatic pressure made their phase transition temperature T _c and the peak temperature T _m decreased, and the frequency dispersion and relaxor behavior enhanced. In these disorder systems of composite pervoskite structures, there appeared polar clusters or nanodomains. The unique physical characteristics, which made the relaxor behavior enhanced, is that the correlation length among these nanodomains decreases greatly with the pressure increasing.     

基于WEB的数字信号处理虚拟系统

基于WEB的虚拟系统为研究数字信号处理提供了一种新的方式,采用虚拟系统对数字信号的频谱分析及语音编码进行研究,通过ASP和ActiveX技术实现系统的动态交互和实时传输,初步实现了信号在网上的实时交互处理,该系统的研究成果已开始应用于教学,科研中。     

Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase

The Diels-Alder reaction, which forms a six-membered ring from an alkene (dienophile) and a 1,3-diene, is synthetically very useful for construction of cyclic products with high regio- and stereoselectivity under mild conditions. It has been applied to the synthesis of complex pharmaceutical and biologically active compounds. Although evidence on natural Diels-Alderases has been accumulated in the biosynthesis of secondary metabolites, there has been no report on the structural details of the natural Diels-Alderases. The function and catalytic mechanism of the natural Diels-Alderase are of great interest owing to the diversity of molecular skeletons in natural Diels-Alder adducts. Here we present the 1.70 A resolution crystal structure of the natural Diels-Alderase, fungal macrophomate synthase (MPS), in complex with pyruvate. The active site of the enzyme is large and hydrophobic, contributing amino acid residues that can hydrogen-bond to the substrate 2-pyrone. These data provide information on the catalytic mechanism of MPS, and suggest that the reaction proceeds via a large-scale structural reorganization of the product.