Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

旋转机械轴心轨迹故障诊断

应用轴心轨迹图的直观性特点，对旋转机械进行状态监测和故障诊断。从轴心轨迹图上判断出振动的前期征兆，对防止故障的恶化和排除故障具有指导作用。因此利用轴心轨迹图来判断旋转机械是否处于正常状态是可行的。     

新型电液激振装置的性能研究

结合电气与液压传动的性能优势，开发出电液激振装置，推导出该装置相关参数的相互关系式。经试验得出，激振装置的振动频率、振幅、压强、流量可以根据需要进行调节。可成为煤矿、冶金、建筑等行业振动设备的一种新型激振装置。     

用知识管理构建电子商务实验教学体系

文章提出用知识管理构建电子商务实验教学体系的观点，就电子商务实验教学体系的建设中，如何构建学习组织、学习系统的思想和方法，进行知识交流与共享．运用知识管理进行实验创新，进行了相应的探讨．     

西部地区科技创新现状及对策研究

本文分析了国内外科技创新形势，对西部地区，特别是陕西、四川、重庆区域的科技创新能力进行重点研究，通过对比指出西部科技创新中所存在的体制、机制方面的问题，提出了西部地区加强科技创新促进经济发展的若干对策。     

桩端压力注浆桩在卵石层中的应用

灌注桩采用桩端压力灌浆新技术后，能大幅度地提高基桩承裁能力，减小其沉降，在工程中已运用多年，但大量工程实践表明其对不同地层的适应性是不同的。根据某工程中钻孔灌注桩现场试验，对桩端压力灌浆技术在卵石层中灌注桩的运用效果其及经济、社会效益进行了分析。     

同源重组在染色体DNA基因克隆中的应用方法

同源重组是生物界普遍存在的现象，从噬菌体、细菌到真核生物都有能发生同源重组的种类。如何把同源重组应用到基因克隆中成为近年来生物学家研究的热点。本文从宿主细胞的改造、重组功能的诱导、感受态细胞的制备、线性同源载体的获得及重组体的鉴定等几个方面具体叙述这种基因克隆的新方法，从分子水平上较为详尽地介绍了同源重组在染色体基因克隆中的应用、前景展望及存在的局限性。     

浅谈电煤价格上涨的必然性

从煤炭的供求现状、市场经济规律、我国电煤价格在备用煤行业及在世界能源产品中价格偏低的状况以及油价的大幅度上升导致煤炭的上涨等方面阐述了我国电煤上涨的必然性。     

以产业化为依托加快太原市无公害蔬菜生产的步伐

针对太原市无公害蔬莱基地的现状及存在问题，阐述了只有以产业化为依托，才能加快太原市无公害蔬莱生产的步伐，并提出了实施无公害蔬莱生产的具体措施。