渗调修复黄瓜陈种子基因组DNA损伤的RAPD研究

用Vc，CaCl2，PEG及其组合对自然老化的黄瓜种子进行渗调处理，利用随机扩增多态性DNA(RAPD)技术对处理前后的种子基因组DNA多态性进行比较分析．结果表明：渗调作用可以修复黄瓜陈种子的DNA损伤．修复效果与种子贮藏时间有关，贮藏半年的种子渗调处理前后RAPD产物差异不明显，贮藏1～2a的陈种子修复的效果好，表现为分子量高的DNA重新合成，RAPD扩增条带增多，亮度增加；贮藏3a的陈种子修复效果不理想。     

商场火灾危险性的评价方法

构造了针对商场类建筑的火灾危险性进行评价的模式·通过实地考察得到商场建筑设计和消防管理方面的具体资料后与《建筑设计防火规范》相对照 ,进行定性的评价 ,在此基础上利用模糊综合评判法 ,对其火灾危险性进行客观的评价·根据具体的结果判断其防火等级 ,得出商场的火灾危险性评价结果 ,针对以上结果提出改进措施·以某商业城为例进行了实际计算 ,结果合理、可行·     

二极管端面泵浦Nd:YAG 946 nm激光器理论分析及其相关量的数值模型

利用激光谐振腔处于稳态时的速率方程导出了准三能级Nd：YAG　946nm起振时透射损耗与最佳激光晶体长度的关系，我们直观地给出了其计算机模拟，在与1064nm透射损耗相比较的基础上，形象给出了一系列情况下的最低镀膜要求(对这个问题系统、细致的讨论在以往的文献中还没有见过)，并且计算机模拟了阈值、输出功率和它们各自相关量的关系，为设计室温下高效运转的946nm激光器提供了必要的理论数值参照．这种分析方法对此类低增益，准三能级或三能级系统有借鉴意义。     

强夯—塑料板排水法在粉土和粉质粘土地基加固中的试验研究

针对黄河中下游粉土、粉质粘土成层地基,通过插设塑料排水板和不插排水板条件下强夯对比试验研究,证实了插设塑料排水板可以有效的降低强夯时超静孔隙水压力峰值,加快其消散,缩短强夯施工工期;提高强夯夯沉,减少振动对土体的扰动,缩短触变恢复时间,因而人工塑料排水板在强夯处理此类地基中是十分有效的。     

C－isotope composition and significance of the Sinian on the Tarim plate

102 samples of limestones,mudrocks shales and tillites from the Sinian in Kuluketage have been analyzed ,Four formations in the Sinian belong to glacial deposits in which the differences in δ^13C are distinct (-14.7‰- 4.2‰),Distinctly negative δ^13C excursions for three times support the viwe of three glacial deposits in this area suggested by previous authors,except for the durations of them ,δ^13C values are generally positive in the Beiyixi Formation of lower part of the Lower Sinian ,representing probably the rock records of a continental rift related to break-up of the Rodinian supercontinent,There are three times of distinct excursions of δ^13C curve in all strata,The first one is from positive values at the bottom to about -5‰ at the top of the Beiyixi Formation ,The second one,from 0‰-3‰ in the Arletonggou Formation of upper part of the Lower Sinian to -3.4‰---14.4‰ in the Teruiaiken Formation.The third one,from positive values in the Zhamoketi Formation of lower part of the Upper Sinian to negative values in glacial varves at the top of the Hankeerqiaoke Formation.     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

新型电液激振装置的性能研究

结合电气与液压传动的性能优势，开发出电液激振装置，推导出该装置相关参数的相互关系式。经试验得出，激振装置的振动频率、振幅、压强、流量可以根据需要进行调节。可成为煤矿、冶金、建筑等行业振动设备的一种新型激振装置。     

用知识管理构建电子商务实验教学体系

文章提出用知识管理构建电子商务实验教学体系的观点，就电子商务实验教学体系的建设中，如何构建学习组织、学习系统的思想和方法，进行知识交流与共享．运用知识管理进行实验创新，进行了相应的探讨．     

西部地区科技创新现状及对策研究

本文分析了国内外科技创新形势，对西部地区，特别是陕西、四川、重庆区域的科技创新能力进行重点研究，通过对比指出西部科技创新中所存在的体制、机制方面的问题，提出了西部地区加强科技创新促进经济发展的若干对策。