基于曲率的自适应曲面数控磨削

利用等包络迹高度控制砂轮行走路径,实现了曲面的自适应数控成型磨削,以提高曲面的磨削效率.首先,根据曲面曲率建立自适应数控成型磨削的数控模式,然后,在比较等间距行走的数控磨削方式的基础上分析曲面曲率不同的成型磨削特征,最后,进行数控成型磨削试验和检测加工曲面的成形误差,验证该自适应数控成型磨削方法的有效性.分析结果表明,利用移动磨削点的包络成型磨削可以减小砂轮磨损对加工精度的影响.此外,等包络迹高度行走的数控磨削可以根据曲面曲率调节行走路经的间距,实现自适应磨削加工,提高曲面的加工效率,而且在加工曲率较大且提高加工精度时可以较大幅度地减少砂轮行走步数.试验结果表明,当等包络迹高度小至1μm时可以形成光顺的加工曲面,在100mm×40mm范围内形状误差PV值可以达到0.285mm.     

用siRNA抑制胃癌细胞AGS中COX-2表达及对AKT磷酸化的抑制

构建了可在真核细胞中表达针对COX-2基因的siRNA表达载体,并用胃腺癌细胞系AGS建立了COX-2 siRNA的稳定转染细胞系。用Western-Blot检测稳定转染细胞系中COX-2的表达以及AKT的磷酸化水平。结果表明稳定转染C0X-2 siRNA的细胞中COX-2的表达较转染空载体的细胞明显降低,且发现COX-2表达的降低可抑制AKT—Ser^473和Thr-^308的磷酸化水平。为进-步探索抑制COX-2对胃癌细胞生物学特性的影响,及其在肿瘤治疗中的作用奠定了基础。     

柔软的黑人性与坚硬的主体性--解读《白宫管家》

《白宫管家》以交叉叙事的方式书写了西塞尔·盖恩斯在种族主义语境中的摇摆人生，以及与之相随的柔软黑人性和坚硬主体性，具体表现在三个方面：第一，棉花田里的舞勺之年，未谙世事的西塞尔以反抗彰显着其黑人性；第二，私刑下的而立与不惑，失去父亲的西塞尔用忍耐守望着自己的主体性，尽量不谈政治；第三，后政治秀的耄耋期颐，失去儿子的西塞尔凭反抗坚守着长期以来其族裔所需的黑人性。实际上，这三段从反抗→忍耐→反抗的人生，是当时黑人在“主人”宰制下的策略生存，旨在以迎合语境所允许个体“奴仆”承载的主体性的前提下守护集体的黑人性。     

基于FPGA的交流伺服系统电流环带宽扩展

在电流环的数学模型基础上,分析了电流环带宽与电流环路延时的关系,比较了几种典型电流环时序下产生的延时,对电流采样和PWM占空比更新时序进行了改进,并在FPGA中得到了具体的验证和实现.实验结果表明:这种改进的电流环时序克服了原有电流环时序的问题,能在不改变功率器件开关频率和不损失输出电压能力的基础上减小电流环路延时,从而提高电流环带宽,改善电流环和速度环的控制性能.     

海子抒情诗中“秋”意象解析

海子诗歌意象丰富，意蕴深厚，极具艺术张力，例如“秋”意象。本文从三方面论述海子抒情诗中“秋”意象。首先，“秋”象征丰收而荒凉的人世；其次，论述“秋”的感伤；最后，“秋”是幻象丛生的国度。     

Optical detection of acetylcholine esterase based on CdTe quantum dots

In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (AChE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10-100 and 100-1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100-2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected AChE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10-140 and 50-1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of AChE.     

Sinorhizobium meliloti lsrB is involved in alfalfa root nodule development and nitrogen-fixing bacteroid differentiation

Rhizobia interact with host legumes to induce the formation of nitrogen-fixing nodules, which is very important in agriculture and ecology. The development of nitrogen-fixing nodules is stringently regulated by host plants and rhizobial symbionts. In our previous work, a new Sinorhizobium meliloti LysR regulator gene (lsrB) was identified to be essential for alfalfa nodulation. However, how this gene is involved in alfalfa nodulation was not yet understood. Here, we found that this gene was associated with prevention of premature nodule senescence and abortive bacteroid formation. Heterogeneous deficient alfalfa root nodules were induced by the in-frame deletion mutant of lsrB (lsrB1-2), which was similar to the plasmid-insertion mutant, lsrB1. Irregular senescence zones earlier appeared in these nodules where bacteroid differentiation was blocked at different stages from microscopy observations. Interestingly, oxidative bursts were observed in these nodules by DAB staining. The decreased expression of lipopolysaccharide core genes (lpsCDE) was correspondingly determined in these nodules. S. meliloti lipopolysaccharide is required for suppression of oxidative bursts or host cell defense. These findings demonstrate that the S. meliloti lsrB gene is involved in alfalfa root nodule development and bacteroid differentiation by suppressing oxidative bursts or defense responses in host cells.     

Discussion on the cantilever casting construction method of concrete crack prevention and control

随着我国建设高峰的来临,悬浇法施工混凝土桥梁越来越多,相伴而来的桥梁混凝土开裂事故也越来越多.通过对施工工艺不当所造成的裂纹原因分析,制定了切实可行的预防及处理措施,对以后同类桥梁施工具有指导意义.     

具阻尼和动态边界的热弹性板的指数衰减性

文章研究一个具有阻尼和动态边界条件的热弹性板的指数衰减性,运用频域方法和偏微分方程的技巧证明了所考虑弹性系统的能量是指数衰减的.     

浅析电力变压器的事故处理

电力变压器是电力系统中很重要的设备,一旦发生事故,将造成很大的经济损失.分析各种电力变压器事故,找出原因,总结出处理事故的办法,把事故损失控制在最小范围内,尽量减少对系统的损害.