条斑紫菜与坛紫菜挥发性风味的电子鼻分析

【目的】利用电子鼻技术区分条斑紫菜(Pyropia yezoensis)与坛紫菜(Pyropia haitanensis)的原藻及其制品。【方法】应用18个金属氧化型气体传感器并通过风味雷达图和主成分分析法对2种紫菜的挥发性风味进行轮廓性分析。【结果】两种方法分析原藻及其制品均显示条斑紫菜和坛紫菜的气味轮廓与敏感物质类型存在差异;相比紫菜原藻,各制品间的气味轮廓较为一致。【结论】电子鼻技术可以区分条斑紫菜与坛紫菜挥发性风味,原藻及其制品均有明显差异。加工过程使得产品挥发性风味趋于统一。     

回转状态下导管螺旋桨水动力特性的数值模拟

采用滑移网格技术和计算流体力学方法,在导管螺旋桨所在的流域内求解其N-S方程,以此模拟在作回转运动的水下机器人流场作用下导管螺旋桨的推力特性,观察螺旋桨在这一环境下的水动力现象.计算结果表明:当来流从尾部流向机器人时,对于尾导管螺旋桨,有机器人影响时其推力系数比无机器人时要小,对处于机器人舷侧的螺旋桨,有机器人时其推力系数比无机器人时要大;当来流从首部流向机器人时其情形刚好相反;而当机器人轴线与流向大致垂直时,有无机器人对螺旋桨推力系数的影响不大.     

上海城市生态环境的对应因子分析

应用对应因子分析法分析上海城市生态环境指标与各样品区的生态环境质量之间的相关程度及其彼此之间的集聚和离散情况⒀结果可分为３类,不同的类区,其生态环境质量受不同生态因子的控制⒀分类情况表明：上海生态环境存在着类似城市空间发展的模式特点,即有着较明显的圈层结构,这同时也说明了对应因子分析法对上海生态环境的规划与建设尤其在绿化生态建设方面具有较强的实际意义⒀文章还将对应因子分析与聚类分析相比较,比较结果两者基本一致     

云南大学2009-2013年获国家自然科学基金资助情况浅析

本文通过对云南大学2009-2013年获国家自然科学基金资助情况的分析,总结了获科学基金资助的优势和存在的不足,并针对不足提出了改进建议.     

Polarity modified triplex DNA by bromide

Conclusion A py-pu-py triplex DNA containing the polarity modification by replacing the atom H5 of cytosine with Br was examined, mainly for the stability of the formation of the triplex DNA. Our computational results show that the conformation of the strand that contained a replaced H5 by cytosine to Br was destabilized, but the Hoogsteen base pairs were stabilized. Although this approach of modifying the polarity of DNA base has not been tried before, it shows the possibility to improve the stability by the polarity modification.     

黔北地区二叠系龙潭组页岩微观孔隙特征及其储气性

为了深入研究黔北地区海陆过渡相页岩储层微观孔隙结构特征,选取了二叠系龙潭组钻井岩心页岩样品进行氩离子抛光-场发射扫描电镜实验、氮气吸脱附实验、及相关地化和等温吸附等测试,研究页岩微观孔隙类型及孔隙结构特征,并探讨微观孔隙对页岩储气性能的影响。研究结果表明,龙潭组页岩储层的孔隙类型主要包括粒间孔、粒内孔、有机质孔、微裂缝4种,以矿物颗粒粒间孔和黏土矿物粒内孔最为发育,有机质孔发育较少,这主要是因为Ⅲ型干酪根显微组分为结构稳定的镜质组和惰质组在生烃过程中不易产生有机质孔;氮气吸附实验结果显示龙潭组页岩孔隙类型复杂,主要表现为两类：细颈广体的墨水瓶孔隙和四边开口的平行狭缝型孔隙,孔径主要介于3～5nm,以发育中孔为主,BET比表面积平均为15.846m2/g,BIH总孔体积平均为0.01258ml/g;龙潭组页岩样品吸附气含量较高（平均为2.37m3/t）,页岩储层微观孔隙对吸附性能有重要的影响,其中吸附气含量与页岩的孔径呈负相关关系,与页岩的比表面积、孔体积呈较好的正相关关系,页岩的纳米级孔隙为烃类气体的赋存和运移提供了有利条件。     

基于生态足迹法的高速公路生态环境影响定量评价——以“绵-遂高速公路”为例

以生态足迹法为基础,从高速公路通过区域的土地转化为可耕地、林地、牧草地、水域、化石能源地、建成地六个主要土地类型入手,阐述高速公路建设对生态环境的影响,并以绵阳——遂宁高速公路（绵阳段）为例,计算高速公路修建前后绵阳市生态足迹与生态容量的变化,并预测评价研究段公路建设在影响范围内对各土地类型的改变的影响程度。为政府部门高速公路建设的提供了决策依据。     

Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis

This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes.     

The mobilization of rat’s mesenchymal stem cells into peripheral blood by LiCL and its potency differentiation

Mesenchymal stem cells （MSCs） are multipotent cells, which can differentiate into different tissues. It is still controversial whether MSCs can be isolated from adult peripheral blood （PB） under normal conditions and whether they can be mobilized in a way similar to that of hematopoieUc stem cells （HSCs）. In this study, rat MSCs circulating in the PB under normal conditions can be isolated and cultured, and MSCs from PB and BM can be mobilized by LiCL. The mobilized MSCs can be induced to differentiate into neuron by such factors as β-mercaptoethanol and supernatants of nerve cell cultures. The present study provides a broader perspective on the repair of neural trauma.