Exocytotic fusion is activated by Rab3a peptides.

Studies of intracellular traffic in yeast and mammalian systems have implicated members of the Rab family of small GTP-binding proteins as regulators of membrane fusion. We have used the patch clamp technique to measure exocytotic fusion events directly and investigate the role of GTP-binding proteins in regulating exocytosis in mast cells. Intracellular perfusion of mast cells with GTP-gamma S is sufficient to trigger complete exocytotic degranulation in the absence of other intracellular messengers. Here we show that GTP is a potent inhibitor of GTP-gamma S-induced degranulation, indicating that sustained activation of a GTP-binding protein is sufficient for membrane fusion. We have found that synthetic oligopeptides, corresponding to part of the effector domain of Rab3a, stimulate complete exocytotic degranulation, similar to that induced by GTP-gamma S. The response is selective for Rab3a sequence and is strictly dependent on Mg2+ and ATP. This suggests that sustained activation of a Rab3 protein causes exocytotic fusion. The peptide response can be accelerated by GDP-beta S, suggesting that Rab3a peptides compete with endogenous Rab3 proteins for a binding site on a target effector protein, which causes fusion on activation.     

环氧乙烷四氢呋喃共聚醚[P(E-CO-T)]的研制

研究了环氧乙烷与四氢呋喃的共聚.使用BF_3络合物为催化剂,1,4-丁二醇为共催化剂.找到了溶液聚合和本体聚合适宜的工艺条件.在此条件下,环齐聚物的生成量为8％左右.找到了方便易行的纯化方法,通过纯化可把聚醚的环齐聚物含量降到1％以下,有效地提高了产品的质量。     

两类C##Fuzzy映象的不动度

本文引入了第(Ⅰ)、(Ⅱ)类 C.—Fuzzy映象的概念,讨论了这两类Fuz—zy映象的不动度问题,得出了几个新结果。使得B.E.Rhoades〔1〕所提出的一个问题在比集值映象及Fuzzy映象更广泛的情形下得到了解决,并且推广了张石生等在文〔2〕中的主要结果。     

腹板波浪度测量仪在桥式起重机检测中的应用

本文介绍武汉钢铁学院研制的QFP-Ⅱ型起重机腹板波浪度测量仪的结构原理,及其在桥式起重机金属结构性能检测中的使用方法,并根据桥式起重机产品质量分等规定的技术要求,给出腹板波浪度分析计算方法。     

B值随机变量序列部分和的矩的收敛速度

本文讨论2阶一致光滑空间及2型空间中随机变量序列部分和的矩的收敛速度问题,所得定理推广了Chow的结果。     

Security Analysis of Discrete Logarithm Based Cryptosystems

0 IntroductionNowthere are many well known cryptosystems based ondiscrete logarithms , such as the ElGamal cryptosys-tem[1]and the digital signature algorithm(DSA)[2]. Discretelogarithms have many advantages . Breaking the discrete log-arithm modulo pri mepappears to be somewhat harder thanfactoringintegern,and elliptic curve cryptosystems may usemuchsmaller keysizesthan RSA-basedcryptosystems of com-parable security.The discrete logarithmproblemover a group can be bro-ken down into a numb…     

高校三标一体管理体系与人本思想

三标一体作为一个完整的、全方位的管理体系富含深刻的人本管理思想,具体而言包括尊重人、关心人、解放人、发展人和依靠人。在“三标一体”管理体系下,高校管理中贯彻以人为本思想必须把握关键。     

技术创新与技校教学浅谈

技术创新具有独特的结构和内含。而职业技校要培养有创新意识的技术工人和技术员，就必须使学生既要掌握理论知识，又要学得生产技能。只有两者有机结合，才能为技术创新打好基础。     

β-桉烯和其衍生物的合成及其抗生育活性

本文叙述了倍半萜类化合物——β-桉烯、衍生物Neointermedeol以及1,2,3,4,7,8-六氢-5,8-二甲基-3-异丙烯基萘的合成。初步药理筛选结果表明,这三个化合物均具有一定的抗生育活性。化合物还未见报道,它的化学结构在本文得到证实。