Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism.     

公开密钥体制与Euler-Fermat定理通俗证明

随着计算机的广泛使用，文件加密解密已成为相当频繁的工作．本文叙述了公开密钥体制中的一种方法，并对其使用的数学工具给出一个通俗证明，以使加密解密工作更加方便、有效．     

虚拟现实与电子商务的结合探讨

提出了一种虚拟现实应用于电子商务的新方法,这种方法能对目前电子商务普遍性问题进行解决,但虚拟技术正处在发展阶段,本方法还有很大的改进空间.     

关于当代大学生自我和谐的思考

从大学生的自我不和谐现状出发,分析影响大学生心理健康的因素,并在此基础上提出了促进大学生自我和谐的几点建议。     

小型LED点阵显示屏实验硬件制作

LED显示屏现在市面上非常流行,本文从实验块的硬件电路出发,讨论了LED点阵显示屏硬件电路的制作,并对实验块进行了成功运行。     

事业单位人事制度改革的思考

论述了事业单位人事制度现状的弊端,提出了应从管理体制上、管理模式上、考核方法上和服务质量上创新的建议。     

李梦生《左传译注》释义商榷

李梦生先生的《左传译注》释义准确,但仍存在一些尚待商榷之处。文章列举了七处,主要涉及字词训释和标点。如“斃”非训“死”,而是训“倒”;“絷”非训“缰绳”,而是训“马绊”;“保”非训“碉堡”,而是训“恃”;“以乘韦先牛十二犒师”不应点断等等。     

BAN逻辑与串空间的比较

研究了2种安全协议的分析方法,即BAN逻辑和串空间.利用这2种方法分别分析了Needham-Schroeder公钥协议,指出了这2种方法各自的优点和缺点.在对这2种方法进行了比较后,指出了这2种方法有2种结合方式.1种是利用串空间给出BAN逻辑的语义,另外1种结合方式是串行,即对于需要分析的协议,先利用BAN逻辑进行分析,然后再利用串空间进行分析,使分析出的结果更加可靠.     

Customer Satisfaction Theory Applied in the Housing Industry： An Empirical Study of Low-Priced Housing in Beijing

Introduction With the housing reform which began in the 1990s in China, the housing industry has grown rapidly in re- cent years, but the quality of housing, especially the quality of low-priced housing, has been recognized to be a problem by government a…     

原子光谱线变宽的因素分析

分别从经典理论和量子理论的不同角度论述了使原子光谱线变宽的各种主要因素:由于能量的不确定关系导致的自然致宽;多普勒致宽;碰撞致宽;因碰撞过程中原子能级变化而导致的压力致宽、场致宽等.通过分析,阐明了实际光谱线与理想光谱线的不同及其原因.