Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

用模拟技术研究材料压缩试验方法

采用LS－DYNA3D非线性动力学有限元分析程序对新材料压缩试验方法进行了计算机模拟，提出了一些改进的实验方法，论证了这种方法的可靠性和可行性。     

新型电液激振装置的性能研究

结合电气与液压传动的性能优势，开发出电液激振装置，推导出该装置相关参数的相互关系式。经试验得出，激振装置的振动频率、振幅、压强、流量可以根据需要进行调节。可成为煤矿、冶金、建筑等行业振动设备的一种新型激振装置。     

纳滤膜材料及其应用

介绍纳滤膜的特点和制备方法，概述纳滤膜在食品、制药、水处理、染料及冶金行业中的应用，并探讨纳滤膜技术的应用前景。     

保护渣性能对结晶器内传热的影响

在实验室模拟研究了结晶器内酸性保护渣的传热情况。结果表明：增加保护渣的粘度、提高保护渣的凝固温度，结晶器与坯壳之间渣膜的传热系数和热流密度都减小而热阻增加，通过调整保护渣的性能，可调节渣膜的传热系数，使其适应连铸坯生产的要求。     

西部地区科技创新现状及对策研究

本文分析了国内外科技创新形势，对西部地区，特别是陕西、四川、重庆区域的科技创新能力进行重点研究，通过对比指出西部科技创新中所存在的体制、机制方面的问题，提出了西部地区加强科技创新促进经济发展的若干对策。     

计算机在高速公路收费系统中的应用及ETC系统设计

对吉林省高速公路收费系统中计算机的应用做了介绍，并根据未来发展的不停车收费系统(ETC)做了介绍，对ECP系统中有关计算机的知识做了论述，以使我省的ETC收费系统有突破性进展。     

三维接触边界元法的一种误差直接估计

将作者所在研究组提出的二维弹性力学问题边界元解误差的直接估计推广到三维问题,给出了确定与域内解连续的边界位移的一种精确有效的方法。在此基础上提出将接触体接触单元间与域内解连续的边界位移之差的某种度量作为三维弹性接触问题边界元法的一种误差直接估计,并且提出了三维弹性接触问题的一种自适应边界元法计算方案。这种方案为确定没有解析解可作比较的复杂接触问题的边界元解精度提供了可能。文中对于三维弹性接触问题,给出了一个计算误差直接估计及自适应边界元法的算例。     

碳布补强含裂纹梁的断裂特性实验

为了解采用纤维复合材料加固含裂纹结构的断裂机理,通过光学焦散线方法对碳纤维布加固含裂纹梁进行3点弯荷载下的断裂实验,研究了其补强效果及断裂特性。实时记录了不同载荷状态下裂纹尖端应力集中引起的焦散斑光学图像,确定了不同初始裂纹长度的试件在粘贴加固不同长度碳布的情况下裂纹尖端的应力强度因子演化规律,分析了初始裂纹和粘贴碳布两种因素对加固效果的影响。实验结果表明:加固碳布可以大幅度地提高含裂纹梁强度,在该文实验中强度最大增加了近2倍。