Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AAF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8. A mixture of cosmid cHT2-derived subclones of the 3' part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated.     

素质教育与21世纪普通高校体育教学改革

体育不仅是素质教育的重要内容，而且也是素质教育的重要手段，21世纪普通高校体育教学要发展、要改革，必须紧紧围绕素质教育进行多角度、全方位地从教学模式、教学目标、课程设置、教学内容、教学方法、教学组织与设计、教学评价和教学环境与氛围等方面的研究、探索与改革，才能适应新世纪中国普通高校与社会发展和人才培养的需要，才能有效地提高学生的身心素质与体育素养为中华民族和全人类培养出符合新世纪需求的、全面发展的、合格的高级建设人才。     

Rapid-time-course miniature and evoked excitatory currents at cerebellar synapses in situ.

Neurotransmission from mossy fibre terminals onto cerebellar granule cells is almost certainly mediated by L-glutamate. By taking advantage of the small soma size, limited number of processes and short dendrite length of granule cells, we have obtained high-resolution recordings of spontaneous miniature excitatory postsynaptic currents (m.e.p.s.cs) and evoked currents in thin cerebellar slices. Miniature currents have a similar time-course and pharmacology to evoked currents and consist of an exceptionally fast non-NMDA (N-methyl-D-aspartate) component (measured rise-time, 200 microseconds; estimated pre-filtered rise-time less than 100 microseconds; decay time constant, tau = 1.0 ms), followed by 50 pS NMDA channel openings that are directly resolvable. We could find no evidence for the recent proposal that miniature currents in granule cells are mediated solely by NMDA channels with a novel time course. The non-NMDA receptor component of m.e.p.s.cs has a skewed amplitude distribution, which suggests potential complications for quantal analysis. The difference in time course between the m.e.p.s.cs reported here and other synaptic currents in the brain could reflect differences in synaptic function or electrotonic filtering; the relative contribution of these possibilities has yet to be established.     

Experimental and Parametric Design of Petroleum Back-pressured Hydraulic Impactor

Percussive-rotary drilling technology was considered many years ago as one of the best approaches for hard rock drilling. It is a key for popularizing this technology on a large scale to design and make an impactor with excellent performance. This paper presents a suit of method to design the percussive parameters for the oil or gas field by introducing the working principle of back-pressured impactor, dividing the working periods of impactor into three phases and establishing the computer emulational model of percussive parameters. It draws a comparison between the results of model calculation and experiment on the basis of analyzing the experiment results of impactor. The conclude provides credible foundation for designing and further ameliorating the impactor.     

在机制CAD班中教学大型通用Ansys分析软件的尝试

阐述了在机制CAD班中利用Ansys平台进行计算机辅助分析CAE的方法和过程，引导学生对有关算例进行了分析．     

生物学实验教学中存在的问题及对策

分析了高师院校生物学实验教学中存在的主要问题，并就如何提高实验教学质量提出了应对措施。     

新时期创新图书馆管理的若干思考

图书馆创新管理是图书馆可持续发展的动力基础,创新图书馆管理要以人为本,实现人本管理与科学管理,提高馆员的素质,培养创新型的人才.     

交际性英语学习策略在英语教学中的实施

交际策略是交际能力的一个重要组成部分.运用一定的教学方法,使学生学会使用交际性英语学习策略,提高学生的交际策略能力,对于学生进行有效交际具有重要作用和意义.