A small GTP-binding protein dissociates from synaptic vesicles during exocytosis.

Low-molecular-weight GTP-binding proteins are strong candidates for regulators of membrane traffic. In yeast, mutations in the sec4 or ypt1 genes encoding small GTP-binding proteins inhibit constitutive membrane flow at the plasma membrane or Golgi complex, respectively. It has been suggested that membrane fusion-fission events are regulated by cycling of small GTP-binding proteins between a membrane-bound and free state, but although most of these small proteins are found in both soluble and tightly membrane-bound forms, there is no direct evidence to support such cycling. In rat brain a small GTP-binding protein, rab3A, is exclusively associated with synaptic vesicles, the secretory organelles of nerve terminals. Here we use isolated nerve terminals to study the fate of rab3A during synaptic vesicle exocytosis. We find that rab3A dissociates quantitatively from the vesicle membrane after Ca2(+)-dependent exocytosis and that this dissociation is partially reversible during recovery after stimulation. These results are direct evidence for an association-dissociation cycle of a small GTP-binding protein during traffic of its host membrane.     

高校年轻干部培养选拔和教育的几点思考

培养和选拔德才兼备的年轻干部是高校改革和发展的重要保证。做好这项工作，必须转变观念，严格把握基本素质要求，健全用人机制，加强教育管理。     

文山州人口素质现状与人才开发的几点思考

从文山州人口的受教育程度可以反映出，文山州目前的人口素质现状是令人担忧的，在此基础平台上，文山州人才资源的学历结构、地域分布、民族分布、行业分布等存在着严重的不平衡，这种状况，极大地制约着文山经济社会的发展。为此，开发文山人才资源，尤其是盘活文山现有人才资源就具有重要的战略意义。     

论思辨与创造想象互补的科学创造之基本思维形式--兼评几种流行的成对思维形式互补的方法论模式

思辨援引高层观念作为创造思维的出发点和孕育新思想的观念母体，并紧紧依靠逻辑的拐杖；创造想象为观念(高层观念、创造观念、创生观念)与经验事实的结合提供形象推理模型，二者互补构成科学创造思维的实质程序和基本思维形式。左脑思维与右脑思维互补、收敛思维与发散思维互补、纵向思维与横向思维互补等等不足以成为创造的基本思维形式，而只能算是某种思维方法或创造技法，原因在于它们是彼此分离的历时互补，在于它们不关注创造内容之运动。     

我国刑事立法中有关被告人诉讼权利的缺失

随着世界各国诉讼机制的日趋成熟，国际社会对人权保障问题越来越关注，我们可以看出在我国刑事立法中对于保障被告人诉讼权利的规定存在不足。根据目前司法实践中出现的几种严重问题，我们应从立法上明确保障被告人所享有的诉讼权利。     

基于 FEM 的深基础工程数值模拟及其实现

简要介绍了有限元法的原理和解题步骤,在借鉴国内外众多有限元分析软件包优点的基础上,开发了自身的FEM三维非线性程序,并用所研发的程序对具体的工程进行了应用和模拟,与实测数据的逼近表明了整个软件系统的准确性和实用性。     

对大学物理课程教学评价的思考

课程改革与课程评价是学校教学中不可缺少的相互联系的两项工作,其目的是形成良性互动以提高教学质量。论述了大学物理教学课程改革与课程评价的目标,分析了在实施过程中应注意的几个问题。     

基于IP多播的机房信息管理系统的设计与实现

提出了一种基于IP多播技术的解决方案并加以实现,满足了对上机学生按系或班级分组进行管理与各类统计的需要,实现了传统C/S结构所能实现的全部功能,解决了传统机房信息管理系统使用带来的问题。     

Genotoxic effects of dithane M-45 on the bone marrow cells of mice in vivo

Genotoxic effects of dithane M-45 were studied on the bone marrow cells of male albino mice (Lacca strain) in vivo. Different doses (30 mg, 40 mg and 300 mg/kg b.wt) of dithane M-45 were injected intraperitoneally and their effects were investigated after time intervals of 1, 2, 5 and 10 days. The chromosomal aberrations observed in the bone marrow cells of male mice after treatment with dithane M-45 were fragments, rings, dicentric chromosomes, terminal chromatid deletions, chromatid gaps and breaks. In addition to these chromosomal aberrations, physiological effects such as uneven stretching of chromatin material, end-to-end chromosomal associations, exchange configurations, clumping, stickiness and centromeric associations were also observed.     

内蒙古鸟纲动物一新记录种——勺鸡

2000年10月在内蒙古赤峰市南部山地获得雌雄勺鸡pucrasiamacrolopha标本各一只,系内蒙古新记录种,属河北亚种P.m.xanthospila.该地区是目前已知勺鸡分布区的最北界,也是勺鸡分布区年均气温最低、年降水量最少、无霜期最短的地区.