论高校图书馆对学生读者不良行为的矫正

论述了高校图书馆对学生读者不良行为矫正发生教育作用的心理学机制,分析了影响矫正教育效果的各种因素,并提出了相应的措施。     

试析图书馆人力资源外包的可行性

通过对图书馆人事管理现状的分析,简述了图书馆人力资源管理外包的重要性,重点探讨了外包的模式和存在的问题,提出了解决问题的对策。     

原子核液-气相变的实验观察

1IntroductionPhase transition and critical phenomenon are extensively debatable subjects in the natural sciences.Re-cently,the same concept was introduced into the astronomical objects[1]as well as the microscopic systems,such as in atomic cluster[2]and n…     

案例教学法在数据库系统概论中的应用

针对数据库系统概论教学中存在的问题,从采用案例教学法的必要性、案例的选择、具体的教学方法等各个方面阐述了案例教学法在该门课程中的重要作用。     

求解DRS多目标优化问题的新颖ε-支配进化算法

首先分析ORS(dominance resistant solutions)多目标优化问题的特点,证明基于Pareto-支配关系的多目标优化问题算法求解该类问题很难收敛.然后,提出一种新的基于ε-支配关系的进化算法-ε-支配进化算法(EDMOEA),给出该算法框架和详细流程.最后,将ε-支配进化算法和NSGA-Ⅱ算法应用于求解一组典型的DRS多目标优化问题和常用的多目标优化测试问题,基于算法的收敛性和Pareto最优解集分布性进行评价和比较分析,表明ε-支配进化算法的有效性.     

RZWQM模型介绍及其应用进展

首先总述了美国RZWQM的研制过程、模拟功能以及模拟的流程,随后分别介绍了RZWQM 6个主要过程:物理过程、土壤化学过程、养分过程、杀虫剂过程、作物生长过程和管理过程;讨论了模型应用过程的注意事项,并探讨了RZWQM98新版本特征;最后对RZWQM模型的应用进展进行了综述,认为RZWQM在模拟作物生长和水分、养分和杀虫剂在作物根区内的运移是很有用的工具。     

Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes, heart ischemia, and cancer

The fraction of pyruvate dehydrogenase complex (PDC) in the active form is reduced by the activities of dedicated PD kinase isozymes (PDK1, PDK2, PDK3 and PDK4). Via binding to the inner lipoyl domain (L2) of the dihydrolipoyl acetyltransferase (E2 60mer), PDK rapidly access their E2-bound PD substrate. The E2-enhanced activity of the widely distributed PDK2 is limited by dissociation of ADP from its C-terminal catalytic domain, and this is further slowed by pyruvate binding to the N-terminal regulatory (R) domain. Via the reverse of the PDC reaction, NADH and acetyl-CoA reductively acetylate lipoyl group of L2, which binds to the R domain and stimulates PDK2 activity by speeding up ADP dissociation. Activation of PDC by synthetic PDK inhibitors binding at the pyruvate or lipoyl binding sites decreased damage during heart ischemia and lowered blood glucose in insulin-resistant animals. PDC activation also triggers apoptosis in cancer cells that selectively convert glucose to lactate. Received 25 August 2006; received after revision 20 November 2006; accepted 20 December 2006     

Mutations in the gene encoding the basal body protein RPGRIP1L, a nephrocystin-4 interactor, cause Joubert syndrome

Protein-protein interaction analyses have uncovered a ciliary and basal body protein network that, when disrupted, can result in nephronophthisis (NPHP), Leber congenital amaurosis, Senior-L?ken syndrome (SLSN) or Joubert syndrome (JBTS). However, details of the molecular mechanisms underlying these disorders remain poorly understood. RPGRIP1-like protein (RPGRIP1L) is a homolog of RPGRIP1 (RPGR-interacting protein 1), a ciliary protein defective in Leber congenital amaurosis. We show that RPGRIP1L interacts with nephrocystin-4 and that mutations in the gene encoding nephrocystin-4 (NPHP4) that are known to cause SLSN disrupt this interaction. RPGRIP1L is ubiquitously expressed, and its protein product localizes to basal bodies. Therefore, we analyzed RPGRIP1L as a candidate gene for JBTS and identified loss-of-function mutations in three families with typical JBTS, including the characteristic mid-hindbrain malformation. This work identifies RPGRIP1L as a gene responsible for JBTS and establishes a central role for cilia and basal bodies in the pathophysiology of this disorder.     

Association scan of 14,500 nonsynonymous SNPs in four diseases identifies autoimmunity variants

We have genotyped 14,436 nonsynonymous SNPs (nsSNPs) and 897 major histocompatibility complex (MHC) tag SNPs from 1,000 independent cases of ankylosing spondylitis (AS), autoimmune thyroid disease (AITD), multiple sclerosis (MS) and breast cancer (BC). Comparing these data against a common control dataset derived from 1,500 randomly selected healthy British individuals, we report initial association and independent replication in a North American sample of two new loci related to ankylosing spondylitis, ARTS1 and IL23R, and confirmation of the previously reported association of AITD with TSHR and FCRL3. These findings, enabled in part by increased statistical power resulting from the expansion of the control reference group to include individuals from the other disease groups, highlight notable new possibilities for autoimmune regulation and suggest that IL23R may be a common susceptibility factor for the major 'seronegative' diseases.     

Improving the thermal stability of lactate oxidase by directed evolution

Lactate oxidase is used in biosensors to measure the concentration of lactate in the blood and other body fluids. Increasing the thermostability of lactate oxidase can significantly prolong the lifetime of these biosensors. We have previously obtained a variant of lactate oxidase from Aerococcus viridans with two mutations (E160G/V198I) that is significantly more thermostable than the wild-type enzyme. Here we have attempted to further improve the thermostability of E160G/V198I lactate oxidase using directed evolution. We made a mutant lactate oxidase gene library by applying error-prone PCR and DNA shuffling, and screened for thermostable mutant lactate oxidase using a plate-based assay. After three rounds of screening we obtained a thermostable mutant lactate oxidase, which has six mutations (E160G/V198I/G36S/T103S/A232S/F277Y). The half-life of this lactate oxidase at 70 °C was about 2 times that of E160G/V198I and about 36 times that of the wild-type enzyme. The amino acid mutation process suggests that the combined neutral mutations are important in protein evolution. Received 15 September 2006; received after revision 21 October 2006; accepted 2 November 2006