Effect of volatile anesthetics on the circular dichroism of bilirubin bound to human serum albumin

The characteristic circular dichroism of bilirubin bound to human serum albumin undergoes a remarkable sign inversion on addition of halothane, chloroform and other volatile anesthetics. This sign inversion, which is completely reversed by removal of the anesthetic, reflects a pronounced conformational change of the bound ligand; probably a complete inversion of chirality. The observation suggests that association of volatile anesthetics with proteins can markedly alter the internal topography of receptor sites and potentially influence the stereoselectivity of ligand binding.     

新型全固态铁离子敏感电极研究

采用稀土电极材料六硼化镧制备了一种新的全固态铁离子敏感电极。研究了该敏感电极在常见介质中对Ｆｅ３＋的响应行为。结果表明，该铁离子敏感电极在１．５ｍｏｌ／ＬＨ３ＰＯ４介质中的线性响应范围、检测下限和响应斜率分别为１．０×１０－５～１．０×１０－１ｍｏｌ／ＬＦｅ３＋，６．０×１０－６ｍｏｌ／ＬＦｅ３＋和６０ｍＶ／ｐＣ，且具有较好的选择性。     

A calcium sensor in the sodium channel modulates cardiac excitability.

Sodium channels are principal molecular determinants responsible for myocardial conduction and maintenance of the cardiac rhythm. Calcium ions (Ca2+) have a fundamental role in the coupling of cardiac myocyte excitation and contraction, yet mechanisms whereby intracellular Ca2+ may directly modulate Na channel function have yet to be identified. Here we show that calmodulin (CaM), a ubiquitous Ca2+-sensing protein, binds to the carboxy-terminal 'IQ' domain of the human cardiac Na channel (hH1) in a Ca2+-dependent manner. This binding interaction significantly enhances slow inactivation-a channel-gating process linked to life-threatening idiopathic ventricular arrhythmias. Mutations targeted to the IQ domain disrupted CaM binding and eliminated Ca2+/CaM-dependent slow inactivation, whereas the gating effects of Ca2+/CaM were restored by intracellular application of a peptide modelled after the IQ domain. A naturally occurring mutation (A1924T) in the IQ domain altered hH1 function in a manner characteristic of the Brugada arrhythmia syndrome, but at the same time inhibited slow inactivation induced by Ca2+/CaM, yielding a clinically benign (arrhythmia free) phenotype.     

Host cell reactivation of ozone-treated T3 bacteriophage by different strains ofEscherichia coli

Summary Host cell reactivation capacity for ozone T3 phage was determined for differentE. coli strains deficient in one or more of the DNA repair mechanisms. The results indicate that DNA polymerase I appears to play a key role in the repair of damage produced on the DNA by ozone while thelexA gene product seems to play a minor one.This research was sponsored by the National Sciences and Engineering Council of Canada. One of us (PLH) acknowledges a scholarship from the NSERCof Canada.     

Increased assembly of cytoskeletal proteins associated with the transformation of human liver cells into fibroblast-like cells.

A topoisomerase II inhibitor, novobiocin, and a deacetylase inhibitor, butyrate, synergistically transformed human liver cells into fibroblast-like cells. This morphological change was associated with an increased production of procollagen type III peptide and a simultaneous assembly of actin, tubulin, vimentin and cytokeratin. Novobiocin and butyrate had no marked effect on the phosphorylation state of cytokeratin proteins, but synergistically enhanced [3H]acetate uptake. From these results, it can be speculated that protein acetylation plays an important role in inducing the assembly of cytoskeletal proteins and the morphological transformation of human liver cells.     

Purealidin A,a new cytotoxic bromotyrosine-derived alkaloid from the Okinawan marine spongePsammaplysilla purea

Summary A new bromotyrosine-derived alkaloid with antileukemic activity, purealidin A (5), has been isolated from the Okinawan marine spongePsammaplysilla purea and its chemical structure elucidated on the basis of the spectroscopic data.     

Experimental Observables on Nuclear Liquid Gas Phase Transition

Progress on nuclear liquid gas phase transition （LGPT） or critical behavior has been simply reviewed and some signals of LGPT in heavy ion collisions, especially in NIMROD data, are focused. These signals include the power-law charge distribution, the largest fluctuation of the fragment observables, the nuclear Zipf law, caloric curve and critical exponent analysis etc.     

Rare Earth Doped Polymer Waveguide Materials and Devices

1 Results Rare earth doped waveguide amplifiers and devices have been demonstrated in silica, crystal and other glass hosts. These rare earth doped optical waveguide devices are based on inorganic materials. Many processing steps are required and can lead to long fabrication time and low yield.Polymer materials offer many distinct properties compared to inorganic materials, such as ease of fabrication, low production costs, simple processing steps, and compatibility with micro-fabrication technologies. ...     

一种针对加性空域掩密算法的通用掩密分析技术

为了提高通用掩密分析技术的检测性能，针对基于加性噪声的空域掩密技术设计了一种通用掩密分析算法，通过分析LSB、＋-k（k=1，3，5）和SM算法所引入的掩密噪声对图像差分直方图产生的影响，提出分别利用差分直方图分布的高阶统计矩和差分直方图曲线下特殊区域之间的面积比值作为分类特征的掩密分析特征选择算法，分别利用该算法和Farid的通用算法对由LSB、＋-k（k=1，3，5）算法掩密后的图像进行检测，实验结果表明，对于不同的嵌入消息比例，该算法的掩密检测率较Farid的算法都有了大幅度的提高。     

异丙酚用于喉部手术前气管切开的临床观察

目的 观察异丙酚在喉部手术前行气管切开期间应用的可行性及安全性.方法 选择择期喉部手术患者24例,每组12例,随机分为异丙酚组(Ⅰ组)和局麻组(Ⅱ组).Ⅰ组患者在局麻前给予异丙酚1～2 mg/kg缓慢静脉注射,待患者睫毛反射消失、意识丧失后给予1%利多卡因局部浸润再行气管切开.Ⅱ组患者仅在1%利多卡因局部浸润下行气管切开.记录切皮前、切皮时、插管时、插管后1 min、插管后3 min时患者心率(HR)、平均动脉压(MAP)及脉搏氧饱和度(SPO2),观察患者呼吸抑制发生率,燥动发生率及术后遗忘率.结果 切皮时,插管时及插管后1 min,3 min Ⅱ组患者心率(HR)、平均动脉压(MAP)较Ⅰ组患者明显升高,躁动发生率Ⅱ组患者明显高于Ⅰ组患者(P＜0.05),呼吸抑制发生率及脉搏氧饱和度(SPO2)变化无明显差异(P＞0.05).结论 异丙酚用于喉部手术前气管切开安全可行.