不同胁迫下盐生杜氏藻3-磷酸甘油脱氢酶基因表达及其与甘油合成的响应

在已克隆的盐藻（Dunaliella salina）3-磷酸甘油脱氢酶GPD基因的基础上,利用实时荧光定量PCR的方法,研究了经高盐、厌氧、磷酸盐以及氧化等不同外界胁迫条件下该基因的表达情况,并同时监测了盐藻细胞甘油合成的动态变化.对比酿酒酵母的GPD基因,发现D.salina GPD基因受高渗诱导,同时D.salina细胞内可能存在多个形式的GPD基因.     

溶剂乙醇对斑马鱼胚胎发育的影响

作为一种提取生物活性成分的常用有机溶剂,乙醇是药物筛选中溶解水溶性较差样品的候选助溶剂。本文研究了不同浓度乙醇对斑马鱼胚胎存活、循环系统发育、血管生成等的影响,为其作为溶剂在斑马鱼模型抗血管生成药物筛选中的应用提供参考。结果显示,0.01%乙醇不会造成胚胎的死亡和仔鱼循环系统畸形,0.1%乙醇引起仔鱼心囊水肿,乙醇浓度达到1%时出现胚胎死亡。低于或等于1%乙醇对仔鱼体节间血管生成无抑制作用。以上结果表明,浓度低于或等于0.01%的乙醇不会影响对被筛样品抗血管生成活性和毒性的评价。     

Helmholtz Solving and Performance Optimization in Global/Regional Assimilation and Prediction System

Despite efficient parallelism in the solution of physical parameterization in the Global/Regional Assimilation and Prediction System(GRAPES), the Helmholtz equation in the dynamic core, with the increase of resolution, can hardly achieve sufficient parallelism in the solving process due to a large amount of communication and irregular access. In this paper, optimizing the Helmholtz equation solution for better performance and higher efficiency has been an urgent task. An optimization scheme for the parallel solution of the Helmholtz equation is proposed in this paper. Specifically, the geometrical multigrid optimization strategy is designed by taking advantage of the data anisotropy of grid points near the pole and the isotropy of those near memory equator in the Helmholtz equation,and the Incomplete LU(ILU) decomposition preconditioner is adopted to speed up the convergence of the improved Generalized Conjugate Residual(GCR), which effectively reduces the number of iterations and the computation time.The overall solving performance of the Helmholtz equation is improved by thread-level parallelism, vectorization, and reuse of data in the cache. The experimental results show that the proposed optimization scheme can effectively eliminate the bottleneck of the Helmholtz equation as regards the solving speed. Considering the test results on a 10-node two-way server, the solution of the Helmholtz equation, compared with the original serial version, is accelerated by 100, with one-third of iterations reduced.     

An MPI+OpenACC-Based PRM Scalar Advection Scheme in the GRAPES Model over a Cluster with Multiple CPUs and GPUs

A moisture advection scheme is an essential module of a numerical weather/climate model representing the horizontal transport of water vapor. The Piecewise Rational Method(PRM) scalar advection scheme in the Global/Regional Assimilation and Prediction System(GRAPES) solves the moisture flux advection equation based on PRM. Computation of the scalar advection involves boundary exchange, and computation of higher bandwidth requirements is complicated and time-consuming in GRAPES. Recently, Graphic...     

几种羟肟酸捕收剂与白云石作用机理研究

为了更好的揭示羟肟酸类捕收剂与白云石作用机理,利用Materials Studio(MS)软件建立羟肟酸捕收剂在白云石矿物表面的吸附模型,通过分子模拟技术模拟计算了真空环境下药剂分子与白云石矿物的相互作用能。从原子尺度揭示了捕收剂在白云石浮选中的捕收机理,研究表明吸附能的差异是导致捕收剂选择性捕收的根本原因。     

英语专业学生话语标记语使用情况之实证研究

通过语料库文本分析的方式,把中国英语学习者（英语专业学生）话语标记语的使用情况和英语本族语者话语标记语的使用情况进行对比。结果显示,中国英语学习者（英语专业学生）在使用频率和使用类型上都有明显差距。教师在教学中应有意识地引导学生注意话语标记语的使用,从而使学生的表达更加流利。     

一类G-框架及其对偶的若干性质

在一个新的Hilbert空间上定义了G-框架及G-对偶框架,并且讨论了他们的性质,改进和推广了其他学者的成果.     

技术经济论证在旅游项目开发中的作用

随着旅游热潮涌动，各地纷纷开发旅游项目，但是大多数旅游项目的开发都缺乏严格的论证。对旅游项目开发进行技术经济论证的主要目的在于为开发规划和投资决策提供科学依据，以提高项目决策的成功率，促进投资决策的科学化。要重点论证开发者的实力和资格，进行旅游资源的调查与评价，做到有的放矢，使旅游开发取得最佳的社会效益、经济效益和环境效益。     

水平管外液膜液滴作用下的蒸汽流动特性

为研究水平管外液体和气体相互作用下的两相流动特性,选择蒸发器中广泛应用的转角正方形排列管束为物理模型,采用流体体积函数(VOF)方法追踪气液界面,提出液体在管间以液滴形式存在的模型假设,结合管间空隙率数据来初始化水平管外液膜厚度和液滴直径,模拟蒸汽在管外液膜和管间液滴作用下的流动过程,分析气液两相的压力场和速度场.结果表明:小喷淋密度下,进出口压降计算值和实验吻合良好;在计算域内,下部区域压力值高于上部区域,且最小压力分布在液滴附近的右下侧区域;压力分布的不均会造成液滴在下落过程中的变形.     

Exploitation of structural and regulatory diversity in glutamate racemases

Glutamate racemase is an enzyme essential to the bacterial cell wall biosynthesis pathway, and has therefore been considered as a target for antibacterial drug discovery. We characterized the glutamate racemases of several pathogenic bacteria using structural and biochemical approaches. Here we describe three distinct mechanisms of regulation for the family of glutamate racemases: allosteric activation by metabolic precursors, kinetic regulation through substrate inhibition, and D-glutamate recycling using a d-amino acid transaminase. In a search for selective inhibitors, we identified a series of uncompetitive inhibitors specifically targeting Helicobacter pylori glutamate racemase that bind to a cryptic allosteric site, and used these inhibitors to probe the mechanistic and dynamic features of the enzyme. These structural, kinetic and mutational studies provide insight into the physiological regulation of these essential enzymes and provide a basis for designing narrow-spectrum antimicrobial agents.