Comparative and statistical analysis of nAChR sequences: An <Emphasis Type="Italic">ab initio</Emphasis> approach to the origin of molecular discrimination

Compared with traditional structure-based approaches for the identification of species-specific ligands, the ab initio approach, based on large-scale protein sequences from different species, has been used to locate specific sites that may be important to the molecular selectivity of species. Statistically significant differences in the distribution of residues in different species and differences in the physicochemical properties of residue-specific sites may largely account for species selectivity. The nicotinic acetylcholine receptor (nAChR), an important neuro-receptor with significantly different ligand selectivity in different species, was used to test our method. Because of the lack of nAChR structural information, the mechanism of ligand discrimination is unclear which hinders attempts at novel molecular design. In this study, the specific site residues 186 and 189 in the principal subunits and residues 34, 55, 56, 57, 106 and 112 in complementary subunits of nAChR were identified by applying our method with stringent statistical cutoffs. These sites were predicted to contribute to ligand selectivity and this result coincides well with the known experimental data.     

霍夫曼水电解器的微型化改进

用医用注射针管作电解室,注射针头作电极,对霍夫曼水电解器进行代换改装,若选用10％的NaOH溶液作电解液,在电源电压12V的条件下4min内可电解出H_24.4ml,Q_22.2ml,两气体体积比符合理论值,验证气体时的现象比较明显,演示效果令人满意。     

剪板机齿轮传动系统降噪的研究

对某剪板机齿轮传动系统振动噪声作了较全面的研究。分析了噪声特性,计算了齿轮传动系统的动力响应,检查了齿轮加工质量等。从而找出了该机主要噪声源,并采取了噪声控制措施,使该机噪声级由93—95dB(A)降低到90dB(A)以下。     

广义各向异性流的渐近性态

对按曲线流γｔ＝Ｖ（θ，ｋ）Ｎ演化着的平面光滑简单闭凸曲线给出了１个等周估计．由此结合尺度论证法得到平面光滑简单闭凸曲线在上述曲线流下的渐近性态．     

Bound lipids in lacustrine sediments in Guchenghu Lake, East China

Conclusion Lipids present in different modes of occurrence in lacustrine sediments vary obviously in their composition. The ester-bound and amide-bound lipids are more likely to be derived from autochthonous input such as bacteria, algae and fungi in the lake, and they were more stable than the free lipids in the process of early diagenesis. Bound lipids record the more actual information about the paleoclimatic and paleoenvironmental changes. Amide-bound P-hydroxy fatty acids can be used as a fingerprint of the past bacterial communities which is informative to the paleoenvironment.     

集线器（HUB）供电技改

介绍了集线器失电的原因,阐述了集线器失电的危害,提出了切实可行的解决方案,提高了变电站运行的安全可靠性。     

Tax Evasion: Models with Self-Audit

Different from traditional tax audit, supervision with self-audit is a combination of audit by the taxpayer himself and audit by the tax authority. This paper mainly studies a taxpayer＇s optimal policy of tax evasion under supervision with self-audit and its related properties, in order to deduce some effective suggestions and theoretical bases to restrain tax evasion. Assuming that only a certain proportion of evaded tax can be discovered when the audit is executed, the authors first formulate a static model with self-audit. This model is divided into two stages. At the first stage, taxpayers declare their taxes, then the tax authority chooses audit objects, based on a known probability, and announces the result; if the taxpayer is chosen, he will enter the second stage, during which he has a chance to pay the evaded tax and the corresponding late fees and then is audited by the tax authority. The authors show the existence and uniqueness of the optimal amount of tax evasion at the first stage and the optimal proportion to self-expose at the second stage. The authors also discuss the related properties of the interior solution, and do elasticity analyses on some parameters. Besides, the authors extend the static model into the corresponding two-period model, and study the existence and uniqueness of the solution of the extended model. Finally, under the assumption that tax evasion can only be discovered with a certain probability when the audit is executed, the authors formulate another static model with self-audit and investigate its properties.     

Vanadyl ions binding to GroEL （HSP60） and inducing its depolymerization

Several vanadium compounds have been known for the hypoglycemic and anticancer effects. However, the mechanisms of the pharmacological and toxicological effects were not clear. In this work, we in- vestigated the potential targets of vanadium in mitochondria. Vanadyl ions were found to bind to mi- tochondria from rat liver with a stoichiometry of 244±58 nmol/mg protein and an apparent dissocia- tion constant (Kd) of (2.0±0.8)×10·16 mol/L. Using size exclusion chromatography, a vanadium-binding protein was isolated and identified to be the 60-kDa heat shock protein (HSP60) by mass spectrometry analysis and immunoassays. Additionally, binding of vanadyl ions was found to result in depolymeri- zation of homo-oligomeric HSP60 (GroEL). HSP60 is an indispensable molecular chaperone and in- volved in many kinds of pathogenesis of inflammatory and autoimmune diseases, e.g. type 1 diabetes. Our results suggested that HSP60 could be a novel important target involved in the biological and/or toxicological effects of vanadium compounds.     

评霍耳片的霍耳电压的有限元算法

本文指出了ＤＥＭＥＹ用有限元法计算ｂ＝２ａ的零耳片的霍耳压的作法是错误的