基于神经网络技术自动筛选Monastrol抑制剂的算法研究

带自动荧光显微镜的HCS（high content screening）系统是一种新兴的显微摄影、筛选和处理系统.HCS系统在摄影过程中会产生大量数据,人工筛选和识别费时费力.本文为了进行Monastrol抑制剂的筛选,基于前馈式神经网络技术研究了一种对HCS系统摄影的大量细胞同时进行特征提取和细胞显型识别的自动算法.在得到各个通道分离的图像后,对不同通道图像进行并行预处理,并采用神经网络和逻辑运算相结合的算法进行处理.我们将该方法运用于Monastrol抑制剂的筛选中,并将结果与人工识别结果进行分析比较.相比较前人提出的multi-phenotypic mitotic analysis（MMA）算法自动识别的正确率得以提高,可更好评估抗癌药剂效用.     

基于小波分析的WiMax网络业务流长相关性

为了精确有效地检测WiMax网络业务流的长相关性,利用自相似随机过程和小波变换都具有尺度不变性的特性,建立了表征长相关性的Hurst参数和小波系数的统计量.研究了业务流长相关性的小波估计法,反复选择小波消失矩和优化尺度参数的范围,减小这些因素对检测结果的影响.利用ON/OFF模型仿真WiMax网络中5种不同类型业务流,然后使用小波估计法对trace文件进行分析.检测分析结果表明:实时业务流的长相关性比较小,非实时业务流的长相关性相对较大;随着网络负载的增大,业务流的长相关性会略有变化,聚合业务流的长相关性的检测值与理论值基本吻合.     

A second generation human haplotype map of over 3.1 million SNPs

We describe the Phase II HapMap, which characterizes over 3.1 million human single nucleotide polymorphisms (SNPs) genotyped in 270 individuals from four geographically diverse populations and includes 25-35% of common SNP variation in the populations surveyed. The map is estimated to capture untyped common variation with an average maximum r2 of between 0.9 and 0.96 depending on population. We demonstrate that the current generation of commercial genome-wide genotyping products captures common Phase II SNPs with an average maximum r2 of up to 0.8 in African and up to 0.95 in non-African populations, and that potential gains in power in association studies can be obtained through imputation. These data also reveal novel aspects of the structure of linkage disequilibrium. We show that 10-30% of pairs of individuals within a population share at least one region of extended genetic identity arising from recent ancestry and that up to 1% of all common variants are untaggable, primarily because they lie within recombination hotspots. We show that recombination rates vary systematically around genes and between genes of different function. Finally, we demonstrate increased differentiation at non-synonymous, compared to synonymous, SNPs, resulting from systematic differences in the strength or efficacy of natural selection between populations.     

Genome-wide detection and characterization of positive selection in human populations

With the advent of dense maps of human genetic variation, it is now possible to detect positive natural selection across the human genome. Here we report an analysis of over 3 million polymorphisms from the International HapMap Project Phase 2 (HapMap2). We used 'long-range haplotype' methods, which were developed to identify alleles segregating in a population that have undergone recent selection, and we also developed new methods that are based on cross-population comparisons to discover alleles that have swept to near-fixation within a population. The analysis reveals more than 300 strong candidate regions. Focusing on the strongest 22 regions, we develop a heuristic for scrutinizing these regions to identify candidate targets of selection. In a complementary analysis, we identify 26 non-synonymous, coding, single nucleotide polymorphisms showing regional evidence of positive selection. Examination of these candidates highlights three cases in which two genes in a common biological process have apparently undergone positive selection in the same population:LARGE and DMD, both related to infection by the Lassa virus, in West Africa;SLC24A5 and SLC45A2, both involved in skin pigmentation, in Europe; and EDAR and EDA2R, both involved in development of hair follicles, in Asia.     

复指数多项式在Hardy空间中的完备性

对复指数多项式在Bamch空间Hpa(1≤ρ≤2)中的完备性给出了充要条件,其中Hρa为开半带形Ia={z=x iy:x＞0,│y│＜a}(a＞0)中的Hardy空间.     

单相有源电力滤波器的控制方法

针对基于单周控制的单相有源电力滤波器只能同时补偿谐波和无功电流、且要求电源电压无畸变的情况,利用基于瞬时无功功率理论的谐波和无功分量检测方法,将单周控制作为电流跟踪控制,在推出控制方程和控制电路的基础上进行了仿真研究,仿真结果证明在电网电压存在畸变的情况下,采用该方案的有源电力滤波器补偿效果良好,而且可以根据需求实现灵活补偿.     

循环网络模拟技术在土石方工程中的应用

土石方工程由于施工过程的复杂及受到众多随机因素的影响,很难用人工方法精确计算各道路的行车及排队情况,确定各道路的等级以及施工机械设备的数量。采用循环网络模拟技术,用挖、装、运、卸等仿真节点来模拟实际施工的相应过程,建立能反映土石方实际开挖过程的仿真模型。仿真得出的一系列施工参数为辅助管理人员做出决策提供参考依据。某工程中的成功应用表明,此方法快捷、便利,值得深入研究并推广应用。     

柔性材料对爆炸载荷的缓冲效应研究

建立了柔性材料桙钢复合壳体在爆炸载荷作用下应力波传播问题的完备方程组;使用有限元方法完成 了对复合壳体在爆炸载荷作用下应力波传播规律的系列数值模拟,并着重讨论了不同炸药层和柔性材料层厚度 对柔性材料与钢交界面载荷的影响。     

实时定量PCR检测B细胞恶性肿瘤中BCL11A基因的表达水平

目的:建立B细胞淋巴瘤/白血病BCL11A基因的定量检测方法,并分析其在B细胞恶性肿瘤中的表达水平。方法:利用实时定量RT-PCR分析B细胞淋巴瘤(18例)、B细胞性慢性淋巴细胞白血病(B-CLL,8例)、T细胞性急性淋巴细胞白血病(T-ALL,8例)和正常对照(15例)外周血单个核细胞(PBMC)中BCL11A基因的表达水平,以甘油醛-3-磷酸脱氢酶(GAPDH)基因作为内对照。结果:B-CLL组和B细胞淋巴瘤组患者PBMC中BCL11A表达水平均明显高于正常对照(P=0.000)和T-ALL组(P=0.000);T-ALL组和正常对照组BCL11A表达水平无显著差别(P=0.084);B-CLL组和B细胞淋巴瘤组BCL11A表达水平无显著差别(P=0.776)。在B细胞淋巴瘤不同的病例中,BCL11A表达水平有较大差异,其中最小值为0.04,最大值为9.70,中位值为1.00。结论:成功建立BCL11A基因的定量检测方法。