Analysis of cDNA sequence, protein structure and expression of parotid secretory protein in pig

Parotid secretory protein (PSP) secreted abundantly in saliva, whose function is related with the anti-bacterial effect. The PSP cDNA has been isolated from pig parotid glands by 3′ and 5′ rapid amplification of cDNA end (RACE), based on the conserved signal peptide region among the known mammalian PSP. The result of homologous comparison shows that pig PSP and human PSP shares the high identity at the level of the primary, secondary and tertiary protein structure. A search for functionally significant protein motifs revealed a unique amino acid sequence pattern consisting of the residues Leu-X(6)-Leu-X(6)-Leu-X(7)-Leu-X(6)-Leu-X(6)-Leu near the amino-terminal portion of the protein, which is important to its function. RT-PCR, Dot blot and Northern blot analysis demonstrated that PSP was strongly expressed in parotid glands, but not in other tissues.     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

基于市场机制的移动Agent系统资源管理

分析了移动Agent的运行机制：移动Agent运行时要消耗所在主机的计算机资源，恶意的移动Agent可以通过大量占用主机资源，引发服务拒绝攻击，因此主机资源的管理是移动Agent系统设计的重要环节。讨论了使用货币的基于市场机制的资源管理策略及主机资源的静态和动态定价策略，同时对实现进行了分析。     

CAN总线接口模块的设计与实现

控制器局域网总线(CAN)是目前较流行的现场总线之一，是一种有效支持分布式控制或实时控制的串行通信网络。由于其高速、高可靠性及独立的设计，被广泛应用与工业现场控制系统中。本以单片机AT89C51和SJA1000控制器为核心组成CAN总线接口模块。介绍硬件原理图和SJA1000的初始化、发送和接收模块程序的设计方法。     

改变水分子簇的结构及诱发的生物效应研究

概述了水分子簇理论研究的基本状况,以及改变水分子团簇结构的4种方法,包括外加磁场、外加电场、激光辐射以及直接加热法;最后简要介绍了改变水分子团簇结构所诱发的生物效应.     

两类自反有向平衡不完全区组设计的存在性

给出了一个递归构造，并且应用这个递归构造给出了区组长为4，v≡2（mod12)和v≡6（mod 12)的SCDB（4，3；v)；这相当于上述两类结构存在性的证明。     

基于网络的多媒体课件的交互设计与实现

基于网络的多媒体课件必须具备交互性的特点，以实现学生与计算机、教师与学生的交互功能，探讨了课件人机交互、教学交互的几种交互方式的设计思路和制作方法，并阐述了基于WEB的B／S模式体系结构的课件设计技术。     

环境教育与化学教学相结合的实践途径

根据中专学校的特点,通过必修课、选修课和课外活动等不同教学形式,使环境教育与中专化学教学相结合,不断提高学生的环保意识和参与能力。     

带有预算费用约束的多地点报童模型

有针对性地研究了一种带有预算费用约束的多地点(连锁店)报童问题。在各地需求变量均服从正态分布的情况下,建立了数学模型,利用最优化方法进行了求解,并对模型的灵敏度进行了分析。