Analysis of biomarkers for the cross-linkage of formaldehyde with bovine serum albumin peptides

Formaldehyde, a well-known environmental toxic hazard, has been found to produce endogenously via semicarbazide-sensitive amine oxidase-catalyzed oxidative deamination of methylamine. In diabetes, the activity of SSAO has been found to increase with a subsequent increase in endogenous formaldehyde production. It has been postulated that SSAO-induced production of formaldehyde may be involved in the alteration of protein structure, which may subsequently cause protein deposition associated with chronic pathological disorders. Formaldehyde has also been found to react （cross-link） with amino group of the N-terminal amino acid residue and with the side-chains of arginine, cysteine, histidine and lysine residues. Therefore, formaldehyde may be responsible, at least in part, for protein cross-linkage, oxidative stress and cytotoxicity. The cross-linking of formaldehyde with bovine serum albumin was studied using LC-MS and Mascot database. The peptides sequence for control BSA （untreated） digested with trypsin was matched in the online database search query by exporting the MS/MS data to online MASCOT database. In this way, a total of twenty-seven peptides were matched in the database search query. These twenty-seven peptides were then searched manually in all of the tryptic BSA samples treated with different concentrations of FA that were incubated in different time intervals. Six formaldehyde-treated BSA peptides （FKDLGEEHFK, HLVDEPQNLIK, KVPQVSTPTLVEVSR, RPCFSALTPDETYVPK, LVNELTEFAK, DAFLGSFLYEYSR） were found to be the possible markers for formaldehyde-protein/peptides adducts.     

基于小波的位场数据融合

对已完成区域重、磁力调查的地区,结合油气勘探的需要,在重点区块进一步开展高精度的重、磁力数据采集．将区块高精度的重、磁力数据与早期获得的品质可靠的区域重、磁力数据融合,既是资料积累的需要,也是新一轮的油气勘探中重、磁力勘探资料处理面临的新问题．二维位场数据经小波分解后能够产生不同分辨率的图像,对区域和重点勘探区块的重、磁力数据进行小波分解,并加以组合选择和低频补偿,实现位场数据的融合．模型试验证实了方法技术的有效性,也揭示了位场数据的融合受到插值步长、小波函数以及小波分解阶数的影响．这一技术应用于南海深水区重力资料的处理,取得了十分理想的效果．     

硝基苯胺单克隆抗体的制备和效果评价

为了测定水中硝基苯胺类化合物,进而开发酶联免疫分析法(EL ISA)快速检测方法,制备了抗硝基苯胺单克隆抗体。以合成的硝基苯胺完全抗原,作为免疫原免疫B a lb/c小鼠,采用B细胞杂交瘤技术,经免疫、融合、筛选和克隆等,得到了抗硝基苯胺单克隆抗体。该抗体亚类为IgG1,制备的单克隆抗体腹水效果评价达10-7,与其他硝基苯胺结构类似物无交叉反应。实验结果表明,用本间接竞争EL ISA方法可以精确地检测不同水样中硝基苯胺残留。     

Petrology and mineral chemistry of new ureilites found in Grove Mountains, Antarctica

Petrography and mineral chemistry of two new ureilites, GRV 021512 and 022931, from the Grove Mountains, Antarctica, are reported in this paper. GRV 021512 exhibits a typical ureilite texture, and consists of 48.3% olivine, 9.4% pigeonite and 38.1% carbonaceous interstitial material and/or reduction product. GRV 022931 has a cataclastic porphyritic texture, and consists of 19.1% olivine and 14.1% pigeonite embedded in 66.3% carbonaceous interstitial material and/or reduction product. The coarse-grained olivine and pigeonite in both meteorites have homogeneous cores, and they are FeO-rich within the range of the ferroan subgroup （subgroup I） of olivine-pigeonite ureilites. All olivine grains of the meteorites show reduction zones along boundaries and cracks. Abundant diamond coexists with graphite as graphite-rich patches and veins in the carbonaceous interstitial material. Petrogenesis of both meteorites and the origin of diamond are discussed.     

Analysis of contact melting around a horizontal elliptical cylinder heat source

The process of AT-driven contact melting of the solid phase change material （PCM） around a horizontal elliptical cylinder heat source is analyzed. Aiming at the problem existed in the published literature, namely the thickness of boundary layer tends to be infinite at φ = 90°, and considering the difference of normal angle between the horizontal elliptical cylinder surface and the solid-liquid interface of PCM, a new mathematic model is proposed, and the fundamental equations for the melting process are derived with the film theory. The new pressure distribution inside the boundary layer, the variation law of normal angle of the solid-liquid interface, the thickness of the boundary layer and the relationship between the melting velocity and resultant force are obtained. The solutions of the fundamental equations under different elliptical compression coefficients are analyzed and discussed. It is found that the thickness of the boundary layer obtained by the new model is a finite value and accords with the experimental result at φ = 90°.     

Discovery of hydrothermal venting community at the base of Cambrian barite in Guizhou Province, Western China: Implication for the Cambrian biological explosion

The barite deposit in the Niutitang Formation of Lower Cambrian, in Tianzhu County, Guizhou Province, western China, is a superlarge barite deposit with about 2×10⁹ tons of reserves. Mineral, petrological and geochemical studies reveal that this barite deposit belongs to a hydrothermal sedimentary deposit. Microscopic observations indicate that a lot of algae, sponge spicules and tube-type fossils are well-preserved in this barite section, and moreover, those fossils share most characteristics of the deep-sea hydrothermal venting community in the modern Pacific. We suggest that the hydrothermal venting community was thriving in hydrothermal vent in early Cambrian, and it is of great signi?cance for elucidating the geological background of the “Cambrian explosion”.     

清疣饮对扁平疣患者体液免疫功能的影响

目的 探讨清疣饮对扁平疣患者血清中免疫球蛋白IgG ,IgA 和IgM含量的影响.方法 用琼脂扩散法定量测定血清免疫球蛋白.结果 患者血清IgG ,IgA 和IgM 水平升高.结论 清疣饮可以提高扁平疣患者体液免疫功能.     

人脸识别算法在监狱通道管理中的应用

针对监狱通道管理的特点, 将人脸肤色模型、 模板匹 配及主分量分析等图像处理和统计学习经典算法有效地结合, 开发了实时有效的人脸识别算法模块. 测试结果及分析表明, 该模块在特定应用中对于人脸定位与验证有一定的优越性.     

基于禁忌搜索的认知无线电性能优化算法

传统的认知无线电(CＲ: Cognitive Ｒadio)优化算法大多是在牺牲认知用户带宽和数据传输率的前提下实 现频谱池干扰抑制的。为进一步提高频谱利用率,提出了基于禁忌搜索的优化算法。该算法采用禁忌搜索获 得产生最小 ICI(Inter-Carrier Interference)的最优小波滤波器。仿真结果表明,将该算法与屏蔽相邻子载波算 法、最佳失效子载波算法和基于遗传算法的 ICI 抑制算法相比较,该优化算法能更好地减少系统 ICI 能量,改 善系统 BEＲ(Bit Error Ｒate)。而且,该优化算法不会占用认知用户带宽,也不用牺牲认知用户数据传输率。