Cloning and characterization of 37 kD laminin receptor precursor in pearl oyster, Pinctada fucata

A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor （37 kDa LRP） is isolated from the mantle tissue of pearl oyster, Pinctadafucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long, with a calculated molecular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial ceils of the mantle edge, suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.     

Localization of calmodulin and calmodulin-like protein and their functions in biomineralization in P. fucata

Calmodulin (CaM) and calmodulin-like protein (CaLP) are two proteins involved in biomineralization. Their localizations in Pinctada fucata mantle epithelia were studied by Western blot (WB) analysis of the nuclear/cytosol fraction of primary cultured Pinctada fucata mantle cells and immunogold electron microscopy. The results showed a completely different distribution of these two proteins at the subcellular level. CaM was distributed throughout both the nucleus and cytoplasm of the mantle epithelium but CaLP was distributed only in the cytoplasm. The functions of these two proteins in biomineralization were investigated by shell regeneration. During this process, the expressions of CaM and CaLP were greatly enhanced in different organelles of the mantle epithelium. Overexpression of these two proteins and a mutant of calmodulin-like protein (M-CaLP) that lacks an extra C-terminal tail in MC3T3-E1 promoted the mRNA expression of osteopontin, a biomineralization marker for osteoblasts. All of the results indicated that CaM and CaLP have completely different distributions in the mantle epithelium and affect the biomineralization process at different levels. The extra C-terminal tail of CaLP is important for its functions in biomineralization in Pinctada fucata.     

国外网上技术市场信息服务研究

网上技术市场作为一种技术中介组织,其信息服务的质量至关重要。首先对国外网上技术市场的现状进行了介绍,在此基础上,重点对国外网上技术市场的信息功能和其所提供的增值服务进行了深入、细致的分析。     

模仿的定义、重要性及其分类

目的是总结模仿研究领域的主要研究成就,以为未来的学术研究建立坚实的基础。总结了模仿领域三个关键问题上的研究进展,即模仿的定义、重要性及其分类。与此同时,指出了目前关于模仿研究的三个误区。     

基于邻菲哕啉和醋酸根的三核锰(Ⅱ)配合物的合成、结构和性能研究

以邻菲口罗啉(Phen)与锰盐在少量醋酸存在的CH3CN/CH2Cl2:(ψ=2:1)混合溶剂中,室温挥发的方法培养出浅黄色配合物[Mn3(Phen)2(CH3COO)6(H2O)·H2O],晶体结构分析表明,配合物属于三斜晶系P-1空间群,每个结构单元中含有3个晶体学独立的锰原子,锰原子通过醋酸根的多种桥联模式处在同一直线上,分子间通过氢键组装成三维超分子配合物,同时对配合物的红外和紫外光谱性质也进行了研究.     

光合细菌在水产养殖中应用的研究进展

光合细菌是一类能进行光合作用的特殊微生物,在水产养殖中有广泛的应用.论述了光合细菌的基本特性,并重点介绍了光合细菌在净化水质、饲料添加剂和预防疾病中的相关应用.     

电动助力转向系统对汽车操纵稳定性的影响

采用多体系统动力学(MBD)与计算机辅助控制系统设计(CACSD)相结合的方法,分析电动助力转向(EPS)系统对汽车操纵稳定性的影响.在多体系统动力学(以MSC.ADAMS软件为支撑)基础上建立包括转向系统、前后悬架系统和前后轮胎的整车动力学模型,作为考察EPS系统对整车性能影响的外部环境;在CACSD(以Matlab/Simulink软件为支撑)基础上建立EPS系统控制模型,研究其助力特性和控制策略.经试验验证,联合仿真模型相对误差在6 %以内,准确地反映了整车的实际情况.     

存活心肌的检测

心肌梗塞后是否有活性成为冠心病治疗决策的关键,而检测心肌存活性的金标准-PET又难以在临床广泛使用。国内外大量的研究表明,存活心肌的检测以多巴酚丁胺超声心动图负荷实验、多巴酚丁胺心电图负荷实验及室壁运动分析、心肌声学造影等方法的特异性、敏感性高,且操作简便、迅速、安全可靠,最值得临床推广运用。