Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis

This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes.     

Super-plasticity of Zr<Subscript>64.80</Subscript>Cu<Subscript>14.85</Subscript>Ni<Subscript>10.35</Subscript>Al<Subscript>10</Subscript> bulk metallic glass at room temperature

Generally, bulk metallic glasses （BMGs） exhibit a very limited plastic deformation under a compression load at room temperature, often less than 2% before fracturing. In this letter, through an appropriate choice of BMGs＇ composition, an amorphous rod of Zr64.80Cu14.85Ni10.35Al10 with a diameter of 2 mm was prepared by using copper mold suction casting. X-ray diffraction and differential scanning calorimetry were utilized to determine its structure and thermal stability, and the uniaxial compression test was adopted to study its plastic deformation behavior at room temperature simultaneously. The results showed that the glass transition temperature and onset temperature of the exothermic reaction of the amorphous rod were 646 and 750 K, respectively, and its micro-hardness was 594.7 Hv. During compression, when the engineering strain and engineering stress arrived at 9.05% and 1732 MPa, respectively, i.e., the true strain and true stress reached 9.42% and 1560 MPa, respectively, the amorphous rod started to yield. After yielding, with the increase of load, the strain increased and the glass rod ulti- mately were compressed into flake-like form. Although the maximum engineering strain was larger than 70%, i.e., the maximum true strain exceeded by 120%, the amorphous specimen was not fractured, indicating that it has super-plasticity at room temperature. Through the appropriate choice of composition and optimization of the technological process, flexible BMG with super-plasticity at room temperature could be produced.     

基于MODIS乌昌地区春季地表温度反演

根据新疆春季地表温度的特性,将前人研究反演地表温度的反演上下限(0℃~50℃)调整为(-30℃~50℃)。然后根据学者研究提出的Plank函数的线性简化方法,对MODIS的第31和32热红外波段分别简化为:B31(T)=0.1088T31-23.349,B31(T)=0.0097T32-220.364,从而反演出更接近于乌昌(乌鲁木齐、昌吉)地区春季地表温度的实测数值。     

基于认知无线网络的跨层设计研究

首先讨论认知无线网络跨层设计的挑战,然后提出一种基于推理机的跨层设计方案。最后,总结全文并对未来工作提出展望。     

数学分析课程教学改革的几点尝试

结合数学分析课程教学实际,探讨数学分析课程教学内容的处理和教学方法的更新以及师资队伍的建设。     

Analysis of realizable rational decimated nonuniform filter banks with direct structure

In the design of nonuniform filter banks （NUFBs） with direct structure, the location of each analysis filter, corresponding to the sampling factor satisfying maximal decimation condition, should be set properly to avoid large aliasing. In this paper, a necessary and sufficient condition for the setting of the location of each analysis filter is derived. The NUFBs, we focus on, have rational decimation factors. Based on the derived condition, the frequency support of each analysis filter for the realizable NUFBs can be determined directly in such a way that the analysis filters can extract the corresponding bands of the input signal. This provides a guideline for the design of NUFBs with direct structure in choosing proper locations of analysis filters.     

Cloning, Characterization, and Expression Analysis of Calreticulin from Pearl Oyster Pinctada fucata

Calreticulin is a unique calcium-binding protein with multiple functions mostly located in the sarcoplasmic/endoplasmic reticulum. A large amount of calcium is absorbed from the medium and transported to mineralization sites during biomineralization in pearl oyster. This paper describes the cloning of the full-length cDNA of calreticulin from Pinctada fucata, namely PCRT. PCRT encodes a deduced 414-amino acid protein, which includes a predicted 17- amino acid signal peptide and an endoplasmic reticulum retrieval sequence HDEL. The protein shows 63%-76% sequence identity and shares some common characteristics with calreticulins from other species. Semi-quantitative RT-PCR indicates that PCRT is ubiquitously expressed in all tissues tested with the highest expression in the hemolymph and the mantle. In situ hybridization analysis of PCRT in the mantle showed strong signals in the inner fold, the inner side of middle fold, and the inner side of outer fold of the mantle epithelium, All these results suggest PCRT might be involved in Ca^2＋ transport and storage during oyster biomineralization.     

Observations of passenger flow and verification of a crowd dynamics model

This paper reports observations of passenger flow in the Wuchang railway station in Wuhan, China during the Chinese Traditional Spring Festival in 2006. The data collected are used to verify a crowd dynamics model previously developed. The crowd dynamics model is based on simulating the global movement of each individual under the influence of the surrounding crowd, and the good agreement between the predictions and observations validates the prediction model. The crowd dynamics model suggests that the crowd movement speed is dominated by two factors： the front-back inter-person effect, and the pedestrian＇s self-motive. The first effect gives logarithmic relationship between the crowd speed and crowd density. The second factor depends on the individual motive driven with which people try to divorce themselves from the control of the crowd movement. The prediction model are helpful to guide the design of public traffic systems for effective crowd dispersal.     

GIS-based highway geological hazard information management and decision support system

Based on the practical application of Geology Information System(GIS) throughout the world, combined with the characters of road’s geological hazard and it’s supervision, the paper introduces on the importance of the research on road’s geological hazards information management and decision-making support system. The paper also analyzes the system’s target, the principles and key techniques in developing the system. In the research, we developed the GIS-based road’s geological hazard information management and decision-making support system and applied it to one speedway in the west of China where contains typical geological hazards. The system based on the database of road’s geological hazard on the grounds of spatial graphic information and attribute information. By virtue of the scientific assessment and prediction mathematical model, integrating the GIS’s strongpoint on spatial analyzing, the system is capable of visualizing the regionalization of road according to the geological hazards it contains, and accurately assessing and predicting geological hazards, thus efficiently assists the road construction and management units in the decision making on controlling the geological hazards and reducing the related loss. Biography: WANG Weidong (1971–), male, Associate professor, Ph.D. candidate, research direction: road and railway engineering, management science and engineering.     

Cloning and characterization of 37 kD laminin receptor precursor in pearl oyster, Pinctada fucata

A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor （37 kDa LRP） is isolated from the mantle tissue of pearl oyster, Pinctadafucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long, with a calculated molecular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial ceils of the mantle edge, suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.