双线性离散域模型转化为连续域模型方法的研究

建立了双线性系统不同域内模型的转化原则－Ｖｏｌｔｅｒｒａ（伏尔泰拉）核等价准则。推导出双线性系统从离散域转化为连续域的方法。最后给出一个具体算例，表明本方法的适用性。     

兴澄特钢主要工序电耗和能耗研究

对兴澄特钢的一些主要工序:烧结、炼铁、炼钢和轧钢的能源物质消耗和耗能工质消耗数据进行收集、整理、分析,基于相同的统计和计算方法,得到生产每吨产品的工序电耗、能耗以及折算的全流程吨钢电耗、能耗值.特炼分厂电炉炼钢年产量2002年为79.18万t,2004年为91.60万t,增长15.69%;电炉吨钢冶炼电耗由340.20kW·h·t-1降至166.30kW·h·t-1,降幅51.12%;而全流程吨钢能耗由289.51kgce·t-1上升到615.44kgce·t-1,增长1.13倍.     

利用HILBERT变换识别结构非线性

本文简要地阐明了用Ｈｉｌｂｅｒｔ变换识别结构非线性的基本原理，并说明了其计算 机实现方法。对实测频段外的影响，提出了实用和有效的补正方法。本文对具有间隙 非线性和库仑摩擦非线性的系统分别作了非线件识别的仿真实验，找出其规律性，并 对实际应用中的一些问题作了分析。     

Crystal structure of the anti-viral APOBEC3G catalytic domain and functional implications

The APOBEC family members are involved in diverse biological functions. APOBEC3G restricts the replication of human immunodeficiency virus (HIV), hepatitis B virus and retroelements by cytidine deamination on single-stranded DNA or by RNA binding. Here we report the high-resolution crystal structure of the carboxy-terminal deaminase domain of APOBEC3G (APOBEC3G-CD2) purified from Escherichia coli. The APOBEC3G-CD2 structure has a five-stranded beta-sheet core that is common to all known deaminase structures and closely resembles the structure of another APOBEC protein, APOBEC2 (ref. 5). A comparison of APOBEC3G-CD2 with other deaminase structures shows a structural conservation of the active-site loops that are directly involved in substrate binding. In the X-ray structure, these APOBEC3G active-site loops form a continuous 'substrate groove' around the active centre. The orientation of this putative substrate groove differs markedly (by 90 degrees) from the groove predicted by the NMR structure. We have introduced mutations around the groove, and have identified residues involved in substrate specificity, single-stranded DNA binding and deaminase activity. These results provide a basis for understanding the underlying mechanisms of substrate specificity for the APOBEC family.     

Effect of polymer chain structure and chain distance on luminescent efficiency

Three kinds of PPV-based copolymers were synthesized and characterized. Their luminescent properties were investigated and discussed by PL spectrum, and time-dependence luminescent spectrum in film and solution state. The results show that in the range of our study, the PL intensity and lifetime of luminescent decay increase with the increasing length of flexible segments and the solution diluting, indicating the tendency of the increase of luminescent efficiency.     

原位聚合法制备镉系颜料色母粒的研究

以水为介质，MMA单体在一定条件下，通过原位聚合使镉系颜料微胶囊化，制得超浓缩色母粒．研究了不同温度、不同气氛、不同引发剂及投加量对单体转化率的影响，并对反应机理进行了探讨．     

The APOBEC-2 crystal structure and functional implications for the deaminase AID

APOBEC-2 (APO2) belongs to the family of apolipoprotein B messenger RNA-editing enzyme catalytic (APOBEC) polypeptides, which deaminates mRNA and single-stranded DNA. Different APOBEC members use the same deamination activity to achieve diverse human biological functions. Deamination by an APOBEC protein called activation-induced cytidine deaminase (AID) is critical for generating high-affinity antibodies, and deamination by APOBEC-3 proteins can inhibit retrotransposons and the replication of retroviruses such as human immunodeficiency virus and hepatitis B virus. Here we report the crystal structure of APO2. APO2 forms a rod-shaped tetramer that differs markedly from the square-shaped tetramer of the free nucleotide cytidine deaminase, with which APOBEC proteins share considerable sequence homology. In APO2, two long alpha-helices of a monomer structure prevent the formation of a square-shaped tetramer and facilitate formation of the rod-shaped tetramer via head-to-head interactions of two APO2 dimers. Extensive sequence homology among APOBEC family members allows us to test APO2 structure-based predictions using AID. We show that AID deamination activity is impaired by mutations predicted to interfere with oligomerization and substrate access. The structure suggests how mutations in patients with hyper-IgM-2 syndrome inactivate AID, resulting in defective antibody maturation.     

阳荷氨基酸组成分析与营养价值评价

研究分析阳荷氨基酸的含量和组成,并对其氨基酸营养进行评价.采用氨基酸自动分析仪检测阳荷幼嫩花序及成熟茎叶中各种氨基酸的含量,并用氨基酸比值系数法评价其必需氨基酸的组成.结果表明,阳荷幼嫩花序及成熟茎叶中总氨基酸含量分别为19.83%和7.75%,必需氨基酸占总氨基酸的比例分别为37.17%和37.55%,均接近于WHO/FAO的标准,比值系数分(SRC)分别为72.6和84.02.在各种必需氨基酸中,阳荷幼嫩花序的第一限制氨基酸为蛋氨酸+胱氨酸,阳荷成熟茎叶没有明显的第一限制氨基酸.阳荷中人体必需氨基酸种类比较齐全,且配比合理,说明阳荷是一种氨基酸营养价值很高的食品资源.     

Global diversity and evidence for coevolution of KIR and HLA

The killer immunoglobulin-like receptor (KIR) gene cluster shows extensive genetic diversity, as do the HLA class I loci, which encode ligands for KIR molecules. We genotyped 1,642 individuals from 30 geographically distinct populations to examine population-level evidence for coevolution of these two functionally related but unlinked gene clusters. We observed strong negative correlations between the presence of activating KIR genes and their corresponding HLA ligand groups across populations, especially KIR3DS1 and its putative HLA-B Bw4-80I ligands (r = -0.66, P = 0.038). In contrast, we observed weak positive relationships between the various inhibitory KIR genes and their ligands. We observed a negative correlation between distance from East Africa and frequency of activating KIR genes and their corresponding ligands, suggesting a balance between selection on HLA and KIR loci. Most KIR-HLA genetic association studies indicate a primary influence of activating KIR-HLA genotypes in disease risk; concomitantly, activating receptor-ligand pairs in this study show the strongest signature of coevolution of these two complex genetic systems as compared with inhibitory receptor-ligand pairs.     

分段压裂水平井的温度分布预测模型

温度预测模型是实现基于分布式温度测试(DTS)解释产出剖面的基础,但定量预测低渗气藏压裂水平井温度剖面仍是一个难题。为此,建立了一套考虑多种微热效应的低渗气藏压裂水平井耦合温度预测模型,模拟了一口低渗气藏压裂水平井的温度剖面,分析了温度剖面特征,并采用正交试验分析法评价了压裂水平井温度剖面对不同因素的敏感性。研究结果表明：①压裂水平井温度剖面呈现出不规则的“锯齿状”,任一“锯齿”都对应着一条有效人工裂缝;②各级裂缝处的井筒温降基本上与裂缝半长呈正相关关系;③从趾端到跟端,各级裂缝位置处的井筒温降与裂缝半长的比值(△T/xf) 依次递减;④压裂水平井温度剖面对各因素的敏感性依次为：裂缝半长>产量>地层渗透率>井筒半径>地层孔隙度>裂缝导流能力>水平倾角。该研究成果为实现基于DTS解释压裂水平井产出剖面提供了模型基础和理论支撑,对于压裂水平井改造效果评价和出水位置定量诊断具有重要意义。