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991.
Structural basis of ultraviolet-B perception by UVR8 总被引:2,自引:0,他引:2
Wu D Hu Q Yan Z Chen W Yan C Huang X Zhang J Yang P Deng H Wang J Deng X Shi Y 《Nature》2012,484(7393):214-219
The Arabidopsis thaliana protein UVR8 is a photoreceptor for ultraviolet-B. Upon ultraviolet-B irradiation, UVR8 undergoes an immediate switch from homodimer to monomer, which triggers a signalling pathway for ultraviolet protection. The mechanism by which UVR8 senses ultraviolet-B remains largely unknown. Here we report the crystal structure of UVR8 at 1.8?? resolution, revealing a symmetric homodimer of seven-bladed β-propeller that is devoid of any external cofactor as the chromophore. Arginine residues that stabilize the homodimeric interface, principally Arg?286 and Arg?338, make elaborate intramolecular cation-π interactions with surrounding tryptophan amino acids. Two of these tryptophans, Trp?285 and Trp?233, collectively serve as the ultraviolet-B chromophore. Our structural and biochemical analyses identify the molecular mechanism for UVR8-mediated ultraviolet-B perception, in which ultraviolet-B radiation results in destabilization of the intramolecular cation-π interactions, causing disruption of the critical intermolecular hydrogen bonds mediated by Arg?286 and Arg?338 and subsequent dissociation of the UVR8 homodimer. 相似文献
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介绍了 LDO线性稳压器的系统组成原理,分析了系统的电源电压抑制比(PSRR)以及噪声与电路结构的关系,在此基础上,对LDO的核心电路模块进行了设计,并基于0.5μm标准CMOS工艺,运用Cadence平台进行了模拟仿真和验证.测试结果表明:该LDO的PSRR最低约为-45 dB@1 MHz,最高约为-75 dB@217 Hz;输出电压噪声在10 Hz频率以下约为0.78 μV(P-P),在10 Hz至l00 kHz频率范围内约为0.1 μV(RMS),能满足低噪声和高PSRR应用的要求. 相似文献
994.
为分析某型号电动摩托车车架在振动工况下的结构强度并减轻重量,基于瞬态动力学理论,采用有限元方法,建立车架瞬态分析有限元模型,计算车架在振动工况下的动强度,得到车架在该工况下的应力分布状况,在此基础上结合Hyperworks优化模块建立以车架重量为目标函数的优化模型,在满足强度约束条件下对车架结构进行优化设计。理论计算和实验研究表明:该车架结构强度有一定富裕,在振动工况下安全系数较高,存在一定优化空间,优化后车架结构强度得到一定提高,符合制造工艺,同时车架重量减轻了12.1%,说明该优化方法对车架结构设计有一定指导意义。 相似文献
995.
We have demonstrated preparing and rotating single neutral rubidium atoms in an optical ring lattice generated by a spatial light modulator, inserting two atoms into a single microscopic optical potential efficiently by dynamically reshaping the optical dipole trap, trapping single atoms in a blue detuned optical bottle beam trap, and confining single atoms into the Lamb-Dicke regime by combining red and blue detuned optical potentials. In combination with the manipulation of internal states of single atoms, the study is opening a way for research in the field of quantum information processing and quantum simulation. In this paper we review the past works and discuss the prospects. 相似文献
996.
In this study, photo-Fenton oxidation was applied to degradation of sulfamonomethoxine sodium (SMMS) in aqueous solution. The operation parameters of pH, temperature, and concentrations of H2O2, Fe2+ and SMMS were investigated. The optimum conditions for the photo-Fenton process were determined as follows: [SMMS]=4.53 mg/L, pH 4.0, [H2O2]=0.49 mmol/L, [Fe2+]= 19.51 μmol/L and T=25°C. Under these conditions 98.5% of the SMMS degraded. The kinetics were also studied, and degradation of SMMS by the photo-Fenton process could be described by first-order kinetics. The apparent activation energy was calculated as 23.95 kJ/mol. Mineralization of the process was investigated by measuring the chemical oxygen demand (COD), and the COD decreased by 99% after 120 min. This process could be used as a pretreatment method for wastewater containing sulfamonomethoxine sodium. 相似文献
997.
The 3 mol% yttria stabilized tetragonal zirconia polycrystals (3Y-TZP) powder had three particle size distributions,while the fine one was lower than 100 nm.The 3Y-TZP compact was prepared by dry-press... 相似文献
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