复方三芪提取物对NMDA致大鼠视网膜损伤的保护作用

目的:研究复方三芪提取物对由N-甲基-D天门冬氨酸(NMDA)诱导的大鼠视网膜损伤模型的保护作用.方法:20只SD大鼠随机分为5组:正常对照组、模型组、复方三芪提取物低、中、高剂量组.模型组和给药组大鼠于右眼玻璃体内注射2μL 40 nmol.L-1NMDA,制备视网膜神经元损伤模型.给药组在NMDA注射前7 d起给予复方三芪提取物灌胃(3.903,7.805,15.61 g.kg-1.B.W.);正常对照组和模型组则给予等剂量的生理盐水灌胃.注射NMDA 7 d后静脉取血,测定血清超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,取眼球组织,切片HE染色,观察视网膜厚度,并对视网膜视神经节细胞层(RGCL)神经元进行计数.结果:模型组大鼠RGCL神经元个数为正常组大鼠的51±6%.同模型组相比,复方三芪提取物(3.903,7.805,15.61 g.kg-1.B.W.)能剂量依赖性地增加RGCL神经元个数(P<0.05),依次为正常组的69±6%,89±7%,103±7%.复方三芪提取物(7.805 g.kg-1.B.W.)显著提高大鼠血清SOD活性并降低MDA含量(P<0.001).结论:复方三芪提取物能抑制NMDA诱导的视网膜脂质过氧化,并能阻止大鼠视网膜神经元进行性丢失,对NMDA诱导损伤的大鼠视网膜有修复作用.提示复方三芪提取物可能具有促进神经元的再生作用.     

新型造纸原料洋麻的硫酸盐法制浆及ECF漂白

以洋麻为研究对象,分析了洋麻的化学成分,研究了不同H因子下硫酸盐法制浆(KP)的较优工艺,以及KP浆的ECF漂白性能及较优的漂白工艺.结果表明:洋麻的硝酸-乙醇纤维素含量为45.00%,酸不溶木素含量为16.50%,聚戊糖和抽出物含量均较低,是优良的造纸用原料;KP法蒸煮较优的制浆工艺为H因子1000、活性碱用量20%、硫化度22%,该工艺下细浆得率达44.9%、卡伯值为20.4、残碱质量浓度为11.67 g/L;ECF漂白的较优ODED漂白工艺为OD0,2%EpD1,1.5%,该工艺条件下终漂浆白度可达82.3%ISO.     

MVC设计模式在手机系统SymbianOS中的应用

设计模式在现代软件开发中,用得越来越广泛,MVC模式是其中的典型。首先论述了MVC设计模式的特点,然后分析其在SymbianOS系统应用程序中的应用。     

面向港口复杂装卸系统的动态机会维护策略

针对以不同衰退特征设备组成的港口复杂装卸系统,提出了设备层-系统层交互的动态机会维护策略,以支撑自动化码头的智能维护决策.引入维护效果和外部环境工况,提出了独立设备的综合衰退演化规则,并结合多目标价值理论和动态循环决策模式,建立设备层多目标预知维护规划模型.在此基础上,分析串联设备间的相互依赖性,利用设备停机的组合维护机会,建立了多设备维护优化调度的联合区间轴策略,对关联设备的预知维护作业采取有针对性的作业合并.数值实例结果证明,所提出的方法可显著降低系统整体维护成本,提供具有可行性、实时性、经济性的系统层维护优化方案.     

三峡库区二道河滑坡变形机制研究

受三峡水库蓄水影响,二道河滑坡出现了变形加剧现象。通过监测数据分析和蓄水过程中滑坡渗流及稳定性数值模拟,分析了滑坡的变形演化过程及变形机制。结果表明:二道河滑坡从开始变形到三峡水库不同阶段蓄水的过程中,经历了由推移式转化为牵引式、由悬浮减重型转化为动水压力滞后型的过程,滑坡变形的地质力学模式为蠕滑—拉裂型。对滑坡变形机制转化的分析可供类似滑坡的分析研究参考。     

Precise orbit determination for geostationary satellites with multiple tracking techniques

A new precise orbit determination (POD) strategy based on the combination of satellite laser ranging (SLR) and C-band transfer ranging for geostationary satellites (GEO) is presented.Two approaches to calibrate ranging biases of the C-band ranging system are proposed,namely the two tracking system co-location comparison and the combined POD method,with calibration accuracies estimated to be 0.5 ns and 1 ns respectively.Using data from a C-band tracking network in China,POD experiments indicate that meter-le...     

自动信任协商安全性的形式化分析与验证

为了对自动信任协商(ATN)的安全性进行形式化分析与验证,文中借鉴安全协议的形式化分析方法,提出一种用进程代数Applied π演算对ATN建模并验证其安全性的方法.该方法将ATN形式化为两个协商者进程的并发执行,其中一个协商者的进程就是对其拥有的证书及授权策略的静态建模;ATN的安全性被定义为Applied π演算的观察等价性,从而使该方法不仅能检查授权策略执行的安全性,而且能对协商者的隐私安全进行验证.借助安全协议的自动分析工具ProVerif,文中实现了对ATN安全性的自动分析.实验结果表明,用Applied π演算形式化及验证ATN的安全性是可行的,安全性验证可自动完成,并且效率较高.     

Sinorhizobium meliloti lsrB is involved in alfalfa root nodule development and nitrogen-fixing bacteroid differentiation

Rhizobia interact with host legumes to induce the formation of nitrogen-fixing nodules, which is very important in agriculture and ecology. The development of nitrogen-fixing nodules is stringently regulated by host plants and rhizobial symbionts. In our previous work, a new Sinorhizobium meliloti LysR regulator gene (lsrB) was identified to be essential for alfalfa nodulation. However, how this gene is involved in alfalfa nodulation was not yet understood. Here, we found that this gene was associated with prevention of premature nodule senescence and abortive bacteroid formation. Heterogeneous deficient alfalfa root nodules were induced by the in-frame deletion mutant of lsrB (lsrB1-2), which was similar to the plasmid-insertion mutant, lsrB1. Irregular senescence zones earlier appeared in these nodules where bacteroid differentiation was blocked at different stages from microscopy observations. Interestingly, oxidative bursts were observed in these nodules by DAB staining. The decreased expression of lipopolysaccharide core genes (lpsCDE) was correspondingly determined in these nodules. S. meliloti lipopolysaccharide is required for suppression of oxidative bursts or host cell defense. These findings demonstrate that the S. meliloti lsrB gene is involved in alfalfa root nodule development and bacteroid differentiation by suppressing oxidative bursts or defense responses in host cells.     

Optical detection of acetylcholine esterase based on CdTe quantum dots

In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (AChE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10-100 and 100-1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100-2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected AChE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10-140 and 50-1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of AChE.     

Observational evidences for the speed of the gravity based on the Earth tide

We have found that the current practical Newtonian formula for the gravity tide of the Earth implies a hypothesis that gravity travels at the speed of light; furthermore, we have derived and solved the propagation equation of gravity using the observation data of Earth tides from Shiquanhe and Wushi, after correction of phase lag due to the anelasticity of the Earth, and found that the speeds of gravity are from 0.93 to 1.05 times the speed of light with a relative error of about 5%. This provides first set of strong evidences to show that the speed of gravity is the same as the speed of light.