Non-viral gene carrier mediated short hairpin RNA interference for inhibition of tumor cells growth

A tumor-targeting gene vector G250mAb-PEI-PEG has been prepared by modification of polyethylenimine （PEI） with polyethyleneglycol （PEG） and G250, a monoclonal antibody against the G250 antigen on tumor cell surface. The transfection efficiency was as high as 70% in G250 positive HeLa cells, whereas the transfection efficiency was relatively low （30%） in normal NIH3T3 cells. A plasmid encoding the short hairpin RNA （shRNA） specific for nucleostemin gene （NS） was efficiently transfected into the HeLa cells with this nonviral gene vector. RNA interference down-regulated the expression of NS gene in HeLa cells, inhibited cells proliferation and induced apoptosis. However, the growth and activity of the NIH3T3 cells were not affected under the same treatment. These results indicate that the reported nonviral gene vector, G250mAb-PEI-PEG, can target and efficiently deliver genes into HeLa cells, and has the potential for the cervical cancer treatment.     

A selective area growth double stack active layer electroabsorption modulator integrated with a distributed feedback laser

A new method for fabricating electroabsorption modulator integrated with a distributed feedback laser （EML） was proposed, With the method we fabricated a selective area growth double stack active layer EML （SAG-DSAL-EML）, Through comparing with other fabrication methods of EMLs, the characters and the merits of the new method presented in this paper were discussed.     

无损检测技术在土木工程中的应用

结构的无损检测是土木工程研究的新课题,它可以评价构件的允许负荷以及寿命或剩余寿命;检测在制造过程中产生的结构不完整性及缺陷情况;可以有效地防止建筑物的突然破坏。研究人员通过研究用于结构损伤识别的多传感器信息融合技术、神经网络技术、负荷响应技术、非接触超声换能技术等无损检测技术,对无损检测技术的未来发展进行了展望。     

Intel80C188在CAN总线通信适配卡中的应用

介绍了 80C188的结构功能 ,提出了一种基于 80C188的CAN总线通信适配卡的设计方案 ,给出了通信适配卡的具体设计线路     

一种通用的自平方分形图形并行算法研究及实现

为了利用现有条件有效提高分形图形的生成速度,研究了基于分布式微机环境的分形图形通用并行算法及实现,并结合实例说明了算法与实现的有效性及合理性。     

The Behavior of the Rotor Spun Yarn(OE yarn)with Involving Twist

The rotor spun yarn(OE yarn),twist of which is calledlayering twist,was added a few involving twist,and itsstructure and properties were measured.The experimen-tal results have shown that the morphological structure ofthe rotor spun yarn with modified twist was changed andits tensile properties and abrasion resistance were im-proved.     

Characterizations of Phosphorus - containing Flame - retardant Poly (Ethylene Terephthalate) Copolyester

Copolyesters of phosphorus - containing flame - retardant poly(Ethylene Terephthalate) (PET) have been prepared and characterized with the objection of producing an effective, environmentally friendly, nonfugitive alternatives to halogen flame retardant system for practical application. With means of NMR, DSC, DTA/TGA, SEM and LOI, structure, thermal property and flam-mability are investigated which reveal that the flame -retardant PET when copolymerized with 2 - carboxyethyl (phenylphosphinic) acid (CEPP) confers excellent fire retardancy with LOI > 28, broader thermal degradation temperature and increased char formation.     

Exome sequencing identifies recurrent somatic RAC1 mutations in melanoma

We characterized the mutational landscape of melanoma, the form of skin cancer with the highest mortality rate, by sequencing the exomes of 147 melanomas. Sun-exposed melanomas had markedly more ultraviolet (UV)-like C>T somatic mutations compared to sun-shielded acral, mucosal and uveal melanomas. Among the newly identified cancer genes was PPP6C, encoding a serine/threonine phosphatase, which harbored mutations that clustered in the active site in 12% of sun-exposed melanomas, exclusively in tumors with mutations in BRAF or NRAS. Notably, we identified a recurrent UV-signature, an activating mutation in RAC1 in 9.2% of sun-exposed melanomas. This activating mutation, the third most frequent in our cohort of sun-exposed melanoma after those of BRAF and NRAS, changes Pro29 to serine (RAC1(P29S)) in the highly conserved switch I domain. Crystal structures, and biochemical and functional studies of RAC1(P29S) showed that the alteration releases the conformational restraint conferred by the conserved proline, causes an increased binding of the protein to downstream effectors, and promotes melanocyte proliferation and migration. These findings raise the possibility that pharmacological inhibition of downstream effectors of RAC1 signaling could be of therapeutic benefit.     

Room temperature ferromagnetism of Ni,(Ni,Li),(Ni,N)-doped ZnO thin films

Ni-doped ZnO thin films (Ni concentration up to 10 mol%) were generated on Si (100) substrates by a sol-gel technique. The films showed wurtzite structure and no other phase was found. The chemical state of Ni was found to be bivalent by X-ray photoelectron spectroscopy. The results of magnetic measurements at room temperature indicated that the films were ferromagnetic, and magnetic moment decreased with rise of Ni concentration. The magnetization of Ni (10 mol%)-doped ZnO film annealed in nitrogen was low...     

Pancreatic islet β-cell deficit and glucose intolerance in rats with uninephrectomy

This study was performed to examine the effect of chronic renal impairment and renin-angiotensin system (RAS) activation induced by unilateral nephrectomy (UNX) on the development of pancreatic islet β-cell deficit and glucose intolerance. Sprague-Dawley rats were randomized into three groups: untreated UNX (n = 10), UNX treated with the angiotensin-converting enzyme inhibitor lisinopril (n = 8) and sham operation (n = 10). Blood glucose, serum insulin, renal function and histological changes of kidney and pancreas were examined 8 months postoperation. Compared with the sham rats, UNX rats developed renal impairment, insulin deficiency and glucose intolerance. Histological staining revealed an islet β-cell deficit associated with increased immunoreactivity for angiotensin and angiotensin type 1 receptor in UNX rats. Treatment with lisinopril significantly improved renal dysfunction, hyperglycemia, insulin secretion and islet RAS expression. These data suggest that chronic renal impairment and RAS activation may contribute to islet β-cell loss and glucose intolerance. RAS blockade may therefore prevent these disorders. Received 29 August 2007; received after revision 25 September 2007; accepted 27 September 2007