Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

微波诱导合成5-芳氧亚甲基-2-[5′-(4″-氯代苯基)- 2′-呋喃甲酰胺基]-1,3,4-噁二唑

在微波辐射条件下,由1 芳氧乙酰基 4 [5′ (4″ 氯代苯基) 2′ 呋喃甲酰基]氨基硫脲(1a～1i)在醋酸汞存在下,快速环合合成了一系列5 芳氧亚甲基 2 [5′ (4″ 氯代苯基) 2′ 呋喃甲酰胺基] 1,3,4 二唑(2a～2i).     

应县木塔修缮工程中柱的夹具设计研究

在夹具的设计中，为了精确控制夹具螺栓张力，试验测定了螺栓扭矩与张力的关系，考虑到现场施工的复杂性及木柱表面摩擦系数的离散性，对夹具不同的4种工况进行了抬升力试验。结果表明，采用三_舛组成、每侧3根24mm螺栓的圆筒套夹具，施工方便、安全可靠，能够在不破坏古木柱表面的前提下，达到对木塔不落架整体抬升的目的。     

低含水率沙床的临界起沙风速

针对目前对风沙起动机制的认识有限的现状，采用量钢分析法推导出对湿沙床的起沙有影响的4个无量纲数。经分析得到起沙风速与沙床沙粒粒径和沙床粘结力间的数量关系。通过建立沙床粘结力与含水率、沙粒粒径的关系式，得到临界起沙风速和沙粒粒径，含水率间的定量关系式。认为低含水率沙床的风沙起动风速与颗粒粒径的一次方和沙床含水率的1／4次方成线性关系，此结论与前人的实验结果符合得很好。     

SPF种鸡群的培育

中国农业科学院哈尔滨兽医研究所利用中国 澳大利亚合作项目“SPF禽饲养预保种设施” ,将京白鸡BWE品系经疫病净化 ,生物学敏感性实验和选育培育试验等技术 ,用定期进行疫病监测并淘汰阳性鸡的方法 ,净化特定的病原和抗体。得出净化了禽白血病病毒等 18种特定病原和抗体 ,对新城疫病毒等 9种病原微生物敏感 ,建立了遗传性能稳定和生产性能良好的具有 7个家系的BWEL SPF种鸡群。已生产SPF种蛋5 0余万枚 ,SPF鸡近 2万只 ,大力支援了科学研究和生产和生物制品生产。该项目的完成是在国内首次采用国内普通鸡群 (京白鸡 )为原材料 ,经净…     

专利信息的利用及其Internet获取

对专利信息进行了初步分析，并介绍了通过Internet获取专利信息的方法。     

小鼠胶原诱导性关节炎的研究进展

胶原诱导性关节炎（collagen-induced arthritis,CIA)是最常用的类风湿性关节炎（rheumatoid arthritis,RA)的动物模型。由于其重复性好，易于界定，并被证实对类风湿性关节炎新疗法（诸如最近发展起来的TNF-α中和治疗方法）的发展有用而被广泛接受。胶原诱导性关节炎模型代表了导致关节炎的某些途径。较大面积讶反映了RA的发生情况，现在我们正处于认识，理解免疫识别的结构（例如MHC分子，淋巴细胞受体和Ⅱ型胶原表位）的时代。这些环节对疾病的发生。发展都极为重要。这为对类风湿性关节炎致病机制的理解艏原诱导性关节炎致病机制的进一步研究提供了有价值的工具。     

软土地基深基坑土钉支护技术应用研究

以盐城市中医院病房楼深基坑支护设计和施工为例，就深基坑设计和施工中应该注意的问题，以及土钉墙支护技术在软土地基域的应用作一些研究，为软土地基深基坑工程的设计和施工提供借鉴。