濒危语言资料的记录和留存

濒危语言资料的调查同一般的语言调查相比有其独特性,这是由濒危语言的特点所决定的。所以,对濒危语言资料的记录会同以往的语料收集不尽相同,其记录的目的是为了全面展示一种语言的真实面貌,长久地保存自然的原始语料,为后代留存一份文化遗产的活化石。     

齿轮齿面接触分析TCA技术及发展动态

通过对齿轮齿面接触分析TCA技术(利用电子计算机进行齿面接触区域的分析和修正)的研究,介绍了齿面接触分析TCA技术的基本原理、发展现状以及发展动态,阐明了TCA技术在现代工业生产中重要意义。     

朊圆酵母尿酸酶的基本特性研究

研究了尿酸酶分离纯化条件和pH、温度、底物浓度、一些有机和无机试剂对尿酸酶酶促反应速度的影响.研究结果表明,用Triton X-100及巯基乙醇处理细胞悬液(pH 8.5),再用超声波处理破碎细胞效果最佳.硫酸铵沉淀尿酸酶的最佳浓度为75%.用Phenyl-Sepharose CL-4B疏水层析和Xanthine-agarose亲和层析两步纯化步骤可获得理想纯度的酶制剂.在一定的条件下,尿酸酶的最适pH和酸碱稳定pH范围分别为8.5和5.0~12.最适温度为37℃;热稳定性较好,在50℃处理30 min时     

浅谈对大学生的道德法制教育

道德法律素养是大学生全面素质的重要内容之一,道德法制教育是培育大学生良好的思想品质、提高他们的道德水平和法律意识的有效途径,关注大学生的道德法制教育对构建社会主义和谐社会、建设社会主义现代化的国家具有重要的现实意义和历史意义。     

Remote sensing of spatial patterns of urban renewal using linear spectral mixture analysis: A case of central urban area of Shanghai (1997―2000)

It is very important to integrate remote sensing with urban geography that the spectral mixture analysis technique is applied to urban land cover evolvement and its eco-environmental effect. Urban land cover is mainly composed of complicated artificial materials, which is the key factor to limit the development of the spectral mixture analysis technique. There are two main aspects in which the technique of spectral mixture analysis is applied to urban geography： one is to calculate vegetation fraction; the other is to build quantitative model of the urban impervious surface obtained from the combination between high albedo fraction and low albedo fraction. The technique of spectral mixture analysis is firstly applied to study urban renewal pattern, scale and mode which happened in Shanghai City from 1997 to 2000,     

Climate change and hydrologic process response in the Tarim River Basin over the past 50 years

Climate change and hydrologic process response in the Tarim River Basin over the past 50 years are the focus of more and more researchers＇ attention. In this paper, both temperature and precipitation time series were found to present a monotonic increasing trend using nonparametric tests. Noticeably, a significant step change in both temperature and precipitation time series occurred in 1986. By contrasting the trends of natural water process in headstream and mainstream, we found that it was anthropogenic activities not climate change that caused the river dried up and vegetation degenerated in the lower reaches of Tarim River. The results of gray correlation analysis show that the runoff of higher latitude distributing river is more closely associated with winter snow stocking, while that of lower latitude is more closely related to summer temperature. Runoff in the headstream is more sensitive to precipitation, while that in the mainstream is more sensitive to evaporation. The strong evaporation caused by increasing temperature weakened runoff to some extent in spite of the fact that precipitation increased over the past 50 years.     

上海民俗的泛化及其发展

民俗文化随社会历史的变化而发生变化。都市民俗文化的变迁主要表现“泛化”，泛化的根源在于历史原因、民风变革、心理原因、科技进步等。     

基于时延估计波束形成预处理的实时语音盲信号分离研究

针对现有盲信号分离算法,在真实环境下性能会降低及计算量大而难以实时处理的局限性,提出一种将基于时延估计的波束形成与一种简单的去相关盲分离算法相结合的分离方法.在波束形成中,首先用互功率谱相位法对信号源到达传感器阵列的相对时延进行估计,然后进行延时对消,在此基础上再进行盲信号分离,并将该去相关盲分离算法推广到频域进一步降低计算复杂度.仿真实验表明,该方法简单可行并使得分离效果显著改善.     

朊圆酵母Y0401尿酸酶的基本特性研究

研究了尿酸酶分离纯化条件和pH、温度、底物浓度、一些有机和无机试剂对尿酸酶酶促反应速度的影响.研究结果表明,用Triton X-100及巯基乙醇处理细胞悬液(pH 8.5),再用超声波处理破碎细胞效果最佳.硫酸铵沉淀尿酸酶的最佳浓度为75%.用Phenyl-Sepharose CL-4B疏水层析和Xanthine-agarose亲和层析两步纯化步骤可获得理想纯度的酶制剂.在一定的条件下,尿酸酶的最适pH和酸碱稳定pH范围分别为8.5和5.0～12.最适温度为37℃;热稳定性较好,在50℃处理30 min时活性保持90%,在60℃处理30min时活性仍保持80%.在最适条件下,该酶催化尿酸的米氏常数(Km)为3.34×10-5mol/L.一些金属离子和有机化合物对酶活性的影响也进行了研究.     

大肠杆菌植酸酶appA的融合表达及耐热性研究

提取大肠杆菌(SUGL)基因组DNA,通过PCR方法从基因组扩增得到植酸酶基因ap-pA,测序结果表明该基因的ORF读框包含1440个核苷酸.将该基因重组于大肠杆菌表达载体pET-32a(+)中,导入大肠杆菌BL21(DE3),构建工程菌BL21(DE3)-pET32a-appA.对表达条件进行了优化,在30℃下,以0.1mmol/L IPTG诱导表达植酸酶,表达量达到10%以上.在37℃,用钒钼酸铵法测定了表达的植酸酶活性为40740.7U/g,同时观察不同加热温度对植酸酶活性的影响程度.发现融合表达的